Effect of pH on the interfacial adsorption activity of pulmonary surfactant

Camacho, Luis; Cruz, Antonio; Castro, R.; Casals, Cristina; Pérez‐Gil, Jesús

doi:10.1016/0927-7765(95)01232-x

Cited by 18 publications

(14 citation statements)

References 20 publications

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“…When pulmonary surfactant is instilled into the trachea and into the airways, both interfacial and viscous properties are critical as they impact the propagating velocity of the liquid plug and the amount of deposited material on the nearby tissues [21,22]. There has been numerous studies devoted to pulmonary surfactant interfacial properties [14,15,35,55] and much less related to their rheological properties [27][28][29]. In particular accurate measurements of static shear viscosity (i.e.…”

Section: -Conclusionmentioning

confidence: 99%

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

Thai

Mousseau

Oikonomou

et al. 2019

Colloids and Surfaces B: Biointerfaces

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The role of pulmonary surfactant is to reduce the surface tension in the lungs and to facilitate breathing. Surfactant replacement therapy (SRT) aims at bringing a substitute by instillation into the airways, a technique that has proven to be efficient and lifesaving for preterm infants. Adapting this therapy to adults requires to scale the administered dose to the patient body weight and to increase the lipid concentration, whilst maintaining its surface and flow properties similar. Here, we exploit a magnetic wire-based microrheology technique to measure the viscosity of the exogenous pulmonary surfactant Curosurf® in various experimental conditions. The Curosurf® viscosity is found to increase exponentially with lipid concentration following the Krieger-Dougherty law of colloids. The Krieger-Dougherty behavior also predicts a divergence of the viscosity at the liquid-to-gel transition. For Curosurf® the transition concentration is found close to the concentration at which it is formulated (117 g L -1 versus 80 g L -1 ). This outcome suggests that for SRT the surfactant rheological properties need to be monitored and kept within a certain range. The results found here could help in producing suspensions for respiratory distress syndrome adapted to adults. The present work also demonstrates the potential of the magnetic wire microrheology technique as an accurate tool to explore biological soft matter dynamics.

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Section: -Conclusionmentioning

confidence: 99%

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

Thai

Mousseau

Oikonomou

et al. 2019

Colloids and Surfaces B: Biointerfaces

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“…49 Pulmonary surfactant Curosurf® (Chiesi Pharmaceuticals, Parma, Italy) is a porcine minced pulmonary surfactant extract used in maternity hospitals for the treatment of premature newborns with respiratory distress syndrome. 63 It is produced as an 80 g L -1 whitish dispersion at pH 6.4 and contains phosphatidylcholine (PC) lipids, sphingomyelin(SM), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylglycerol (PG) and the hydrophobic proteins SP-B and SP-C. 50,64,65 Curosurf® is considered as a model biomimetic surfactant because its composition and bulk properties are constant from one batch to another and because of its long-term shelf stability. The Curosurf® formulation is compared to pulmonary surfactant obtained from lung lavage in Supplementary Information S1.…”

Section: Iv1 -Materialsmentioning

confidence: 99%

Effect of Nanoparticles on the Bulk Shear Viscosity of a Lung Surfactant Fluid

et al. 2019

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Inhaled nanoparticles (< 100 nm) reaching the deep lung region first interact with the pulmonary surfactant, a thin lipid film lining the alveolar epithelium. To date, most biophysical studies have focused on particle induced modifications of the film interfacial properties. In comparison, there is less work on the surfactant bulk properties, and on their changes upon particle exposure. Here we study the viscoelastic properties of a biomimetic pulmonary surfactant in the presence of various engineered nanoparticles. The microrheology technique used is based on the remote actuation of micron-sized wires via the application of a rotating magnetic field and on timelapse optical microscopy. It is found that particles strongly interacting with lipid vesicles, such as cationic silica (SiO2, 42 nm) and alumina (Al2O3, 40 nm) induce profound modifications of the surfactant flow properties, even at low concentrations. In particular, we find that silica causes fluidification, while alumina induces a liquid-to-soft solid transition. Both phenomena are described quantitatively and accounted for in the context of colloidal physics models. It is finally suggested that the structure and viscosity changes could impair the fluid reorganization and recirculation occurring during breathing.

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“…Interfacial adsorption was assayed using a specially designed surface balance similar to that described previously (Camacho et al 1996;Cruz et al 1997;Nag et al 1996;PeÂ rez-Gil et al 1992a). This balance contained 1.5 mL of subphase at 21°C, and the change of surface pressure (p) during adsorption (spreading) from vesicles was monitored with a dipping plate of Whatman no.…”

Section: Interfacial Adsorption Assaysmentioning

confidence: 99%

Microstructure and dynamic surface properties of surfactant protein SP-B/dipalmitoylphosphatidylcholine interfacial films spread from lipid-protein bilayers

Cruz¹,

Worthman

Serrano³

et al. 2000

Eur Biophys J

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Suspensions of dipalmitoylphosphatidylcholine (DPPC) bilayers containing 5, 10 or 20% (w/w) surfactant protein SP-B have been reconstituted and spread at air-liquid interfaces. Compression isotherms of DPPC/SP-B monolayers spread from these preparations were qualitatively comparable to the isotherms of the corresponding DPPC/SP-B monolayers spread from solvents. SP-B was squeezed-out at higher pressures from vesicle-spread films than from solvent-spread monolayers. SP-B caused a marked decrease on the rate of relaxation of DPPC collapse phases to equilibrium pressures in all the lipid/protein films assayed. This stabilizing effect was higher in vesicle-spread than in solvent-spread monolayers. Inclusion in the films of traces of the fluorescent probe NBD-PC (1 mol%) and use of a fluorescent derivative of SP-B labeled with a rhodamine derivative, Texas Red, allowed for direct observation of protein and lipid domains at the interface by epifluorescence microscopy. Upon compression, SP-B altered the packing of phospholipids in the bilayer-spread films, observed as a SP-B-induced reduction of the area of liquid-condensed domains, in a way similar to its effect in solvent-spread monolayers. SP-B was not associated with condensed regions of the films. Fluorescence images from vesicle-spread films showed discrete fluorescent aggregates that could be consistent with the existence of lipid-protein vesicles in close association with the monolayer. Both the retention of SP-B at higher surface pressures and the greater stability of collapse phases of DPPC/SP-B films prepared by spreading from liposomes in comparison to those spread from solvents can be interpreted as a consequence of formation of complex bilayer-monolayer interacting systems.

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Effect of pH on the interfacial adsorption activity of pulmonary surfactant

Cited by 18 publications

References 20 publications

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

On the rheology of pulmonary surfactant: Effects of concentration and consequences for the surfactant replacement therapy

Effect of Nanoparticles on the Bulk Shear Viscosity of a Lung Surfactant Fluid

Microstructure and dynamic surface properties of surfactant protein SP-B/dipalmitoylphosphatidylcholine interfacial films spread from lipid-protein bilayers

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