Effect of pH on Pb2+ accumulation in Saccharomyces cerevisiae and Aureobasidium pullulans

Suh, Jung Ho; Yun, Jong-Won; Kim, D. S.

doi:10.1007/pl00009056

Cited by 11 publications

(9 citation statements)

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“…A. pullulans, compared with S. cerevisiae, may have used a different Pb 2+ accumulation process due to the existence of EPS [15]. Thus, we postulated that the increased Pb 2+ accumulation capacity of older biomass was caused by EPS around the cell surface.…”

Section: Effect Of Eps On Pb 2+ Accumulationmentioning

confidence: 96%

Effect of extracellular polymeric substances (EPS) on Pb2+ accumulation by Aureobasidium pullulans

Suh

Yun

Kim

1999

Bioprocess Engineering

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In Pb 2+ accumulation by Aureobasidium pullulans, the time to reach an equilibrium state was not dependent on the initial cell dry weight. The Pb 2+ accumulation capacity was increased from 56.9 to 215.6 mg Pb 2+ /g cell dry weight as the biomass was stored from 1 to 53 days, and correlated with the amount of excreted extracellular polymeric substances (EPS). It was observed that Pb 2+ accumulated only on the surface of the intact cells of A. pullulans due to the existence of EPS, whereas Pb 2+ penetrated into the inner cellular parts of the EPS-extracted cells. IntroductionThe presence of heavy metal ions in municipal wastewater may have detrimental effects on the water puri®cation systems. In the interest of public health protection studies concerning the sources of heavy metal ions, their removal mechanisms in wastewater treatment plants, and the fate of heavy metal ions discharged into surface streams and/or land are deemed necessary. In recent years, the investigation of bioaccumulation/biosorption for the removal of heavy metal ions by microorganisms and their microbial products has been increased [1±3]. Microorganisms bind metal ions via any one (or more) of several known mechanisms, including adsorption, inorganic precipitation, complexation, ion exchange and active transport [4].In particular, Pb 2+ , well recognized for its detrimental effect on the environment where it accumulates throughout the food web, is generated from mining, metal, dyestuff, electric, and petroleum industries, etc. It is well known that Pb 2+ is easily accumulated in microorganisms.For instance, equilibrium metal uptake values using freeze-dried Rhizopus arrhizus increased in the order: Sr 2+ < Mn 2+ < Zn 2+ < Cd 2+ < Cu 2+ < Pb 2+ , and were positively correlated with the covalent index of the metal ions [5]. The work of Leusch et al. [6] indicated that the order of adsorption for metal ions in the biomass particles of Sargassum¯uitans was Pb 2+ > Cd 2+ > Cu 2+ > Ni 2+ > Zn 2+ . It has also been described that fungal biomass is capable of sequestering and accumulating heavy metal ions [7,8]. Fungal pigments are implicated in metal resistance and/or uptake by some fungal cultures. Biosynthesis of metalbinding pigment melanin, produced as a response to copper-induced culture stress, has been studied in a polymorphic fungus Aureobasidium pullulans [9]. Nevertheless, the relationship between the process of heavy metal ion uptake and extracellular polymeric substances (EPS) in fungi has not been fully understood.In the present study, in an attempt to elucidate the detailed process of Pb 2+ accumulation in A. pullulans, the critical effect of EPS was investigated using transmission electron microscope (TEM). Materials and methods Microorganism and growth conditionAureobasidium pullulans KFCC (Korean Foundation of Culture Collection) 110245 was aerobically cultivated with 100 ml medium containing (as g/l) 200, sucrose; 20, yeast extract; 5, K 2 HPO 4 ; 2, MgSO 4 á 7H 2 O; 15, NaNO 3 in a rotary-shaker incubator (150 rpm) at 30°C for 72...

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Section: Effect Of Eps On Pb 2+ Accumulationmentioning

confidence: 96%

Effect of extracellular polymeric substances (EPS) on Pb2+ accumulation by Aureobasidium pullulans

Suh

Yun

Kim

1999

Bioprocess Engineering

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“…Heat treatment followed by ethanol treatment has also increased Cd biosorption capacity by twofold (Ghorbani et al 2008); this enhanced capacity has been explained by an increase in the accessibility of the metal ions to the metal-binding sites on the biomass (Ghorbani et al 2008;Göksungur et al 2005). Alternatively, it has been reported that hot alkali yeast treatment can moderately decrease Cu(II) accumulation when compared with native (untreated) yeast cells (Brady et al 1994a); other authors have also reported that dead biomass has lower Sr(II) (Avery and Tobin 1992) or Pb(II) uptake (Suh and Kim 2000;Suh et al 1998) than the respective live cells. In contrast, heat treatment can enhance the exposure of additional functional groups implicated in the biosorption of heavy metals.…”

Section: Inactivating Processes and Chemical Modification Of The Yeasmentioning

confidence: 99%

Bioremediation of industrial effluents containing heavy metals using brewing cells of Saccharomyces cerevisiae as a green technology: a review

Soares

2011

Environ Sci Pollut Res

129

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The release of heavy metals into the environment, mainly as a consequence of anthropogenic activities, constitutes a worldwide environmental pollution problem. Unlike organic pollutants, heavy metals are not degraded and remain indefinitely in the ecosystem, which poses a different kind of challenge for remediation. It seems that the "best treatment technologies" available may not be completely effective for metal removal or can be expensive; therefore, new methodologies have been proposed for the detoxification of metal-bearing wastewaters. The present work reviews and discusses the advantages of using brewing yeast cells of Saccharomyces cerevisiae in the detoxification of effluents containing heavy metals. The current knowledge of the mechanisms of metal removal by yeast biomass is presented. The use of live or dead biomass and the influence of biomass inactivation on the metal accumulation characteristics are outlined. The role of chemical speciation for predicting and optimising the efficiency of metal removal is highlighted. The problem of biomass separation, after treatment of the effluents, and the use of flocculent characteristics, as an alternative process of cell-liquid separation, are also discussed. The use of yeast cells in the treatment of real effluents to bridge the gap between fundamental and applied studies is presented and updated. The convenient management of the contaminated biomass and the advantages of the selective recovery of heavy metals in the development of a closed cycle without residues (green technology) are critically reviewed.

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“…One part of the biomass was autoclaved (Model MSW-101YDX, India) at 121°C El-Sayed 6901 for 15 min, then dried at 80°C to a constant weight to prepare the dead cells and the other part was directly used as live cells. Cell viability was assessed by conventional spread plate technique using distilled deionized water as a diluent (Suh et al, 1998).…”

Section: Preparation Of Biosorbentmentioning

confidence: 99%

“…Aliquots (50 ml) of cadmium chloride solution prepared at twice the desired concentration (250 mg/L) were added to each flask, and flasks were shaken in an incubator shaker at 28°C and 120 rpm (Suh et al, 1998). The pH was adjusted to 6 with an aqueous solution of 0.1 N HCl and 1 N NaOH.…”

Section: Determination Of Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

The use of Saccharomyces cerevisiae for removing cadmium(II) from aqueous waste solutions

El-Sayed¹

2012

Afr. J. Microbiol. Res.

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The biosorption of cadmium(II) on live and dead biomass of Saccharomyces cerevisiae was investigated with respect to the adsorption conditions. The biosorption mechanism was investigated by energy dispersive X-ray analysis (EDAX) and transmission electron microscope (TEM). The effects of the biosorbent dose, initial metal ion concentration, initial pH and contact time were studied. The optimum conditions for cadmium(II) biosorption were found to be 3 g/L, 250 mg/L and 6.0, respectively, for biosorbent dose, initial metal ion concentration and initial pH. Cadmium(II) biosorption by dead cells was a fast process. Under these conditions, the maximum biosorption capacity of the dead biomass was obtained to be 55 mg/g, while that of live biomass was 36 mg/g. TEM observations indicated the presence of cadmium (II) deposits intracellularly and extracellularly. EDAX examinations showed that cadmium(II) was exchanged with the element (aluminum) present on the surface of native cells of S. cerevisiae, thereby suggesting the occurrence of bioadsorption, ion exchange and complexation. Cadmium(II) biosorption capacity on dead cells was enhanced by ethanol treatment and alginate immobilization. Overall, the results showed that the ethanol-treated, alginate-immobilized biomass was capable of removing cadmium(II) from wastewater samples.

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Effect of pH on Pb2+ accumulation in Saccharomyces cerevisiae and Aureobasidium pullulans

Cited by 11 publications

References 11 publications

Effect of extracellular polymeric substances (EPS) on Pb2+ accumulation by Aureobasidium pullulans

Effect of extracellular polymeric substances (EPS) on Pb2+ accumulation by Aureobasidium pullulans

Bioremediation of industrial effluents containing heavy metals using brewing cells of Saccharomyces cerevisiae as a green technology: a review

The use of Saccharomyces cerevisiae for removing cadmium(II) from aqueous waste solutions

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