Effect of pH on Human Complement Activity

Fogel, Bernard J.; Hook, W. Alexander Van; Fife, Earl H.

doi:10.1038/2101381a0

Cited by 7 publications

(4 citation statements)

References 2 publications

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“…The pH requirement was similar as in most other species; sheep C had its optimum activity at pH 6.5 and a decrease in titres started already after an increase of pH above 7. Many others have reported a decrease at higher pH namely above 7.5 for guinea pig C (MAYER et al, 1946;MAYER, 1961;YACHNIN and RUTHENBERG, 1965), human C (YACHNIN and RUTHENBERG, 1965;FOGEL et al, 1966), bovine C (BARTA and BARTA, 1972), and dog C (BARTA and BARTA, 1973). The only noticeable exception from this rule being horse C with its optimum at p H 9 (BARTA et al, 1973).…”

Section: Discussionmentioning

confidence: 96%

Haemolytic assay of bovine serum complement

Bárta

Barta

1972

Journal of Immunological Methods

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Section: Discussionmentioning

confidence: 96%

Haemolytic assay of bovine serum complement

Bárta

Barta

1972

Journal of Immunological Methods

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“…Complement convertase activity is slightly increased at pH 6.8, 79 while activity is similar at pH 6.5 as at pH 7.4. 79,80 Below this, however, activity quickly becomes inhibited.…”

Section: Are Intracellular Functions Of Complement Compatible With Th...mentioning

confidence: 83%

“…Complement convertase activity is slightly increased at pH 6.8, 79 while activity is similar at pH 6.5 as at pH 7.4. 79 , 80 Below this, however, activity quickly becomes inhibited. Therefore, complement convertases can be expected to be active within early endosomes.…”

Section: Controversies Regarding Intracellular Complementmentioning

confidence: 83%

“…Endosomes, where pools of intracellular C3 or C3a “stores” have been identified, 30,36 have an acidic pH of about 6.5 (early endosomes) to 5.5 (late endosomes). Complement convertase activity is slightly increased at pH 6.8, 79 while activity is similar at pH 6.5 as at pH 7.4 79,80 . Below this, however, activity quickly becomes inhibited.…”

Section: Controversies Regarding Intracellular Complementmentioning

confidence: 87%

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Intracellular complement: Evidence, definitions, controversies, and solutions

King

Blom

2022

Immunological Reviews

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The traditionally understood roles of complement in innate immunity take place in the extracellular environment, directly opsonizing and even lysing some pathogens via the action of C3 deposition and formation of the terminal membrane attack complex 1 but also acting as a danger sensing mechanism, transmitting signals to cells via cell-surface receptors of activated complement components. 2 While these functions have largely been attributed to circulating complement proteins secreted mainly from the liver, it has been recognized that local expression of complement proteins can also have an important role in tissues, 3 for example, in infection 4,5 and in complement-dependent pathologies. [6][7][8] The discovery that some cell types express multiple complement proteins required for complete activation pathways, leading to spontaneous activation, also opened the possibility for autocrine complement activation playing roles in cellular homeostasis, activation, and inflammation. 9,10 For example, more recently, cell-intrinsic C3 expression in fibroblasts has been shown to play an important role in priming tissue sites for inflammation. 11 In autoinflammatory disease, repeated attacks can increase in severity. It was shown that mice injected at the same site with identical doses of sterile inflammatory danger-or pathogenassociated molecular patterns (DAMPs or PAMPs) suffered increasing levels of inflammation, while injection at different sites did not. This was not dependent on the adaptive immune system, as shown using SCID and RAG1-KO mice, but was dependent on C3, as shown using C3-KO mice. In addition, adoptive transfer of exposed fibroblasts from WT but not C3-KO mice "primed" the recipient site for increased inflammation, indicating the importance of fibroblastexpressed C3 in this priming process. This detailed and clear study indicates a role for local, or cell-intrinsic C3 in fibroblasts in sterile inflammation. The exact function of C3 was not however defined,

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Hemolytic assay for goat (caprine) and swine (porcine) complement

Oyekan

Bárta

1980

Veterinary Immunology and Immunopathology

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Effect of pH on Human Complement Activity

Cited by 7 publications

References 2 publications

Haemolytic assay of bovine serum complement

Haemolytic assay of bovine serum complement

Intracellular complement: Evidence, definitions, controversies, and solutions

Hemolytic assay for goat (caprine) and swine (porcine) complement

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