1998
DOI: 10.1016/s0040-6090(98)00587-2
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Effect of pH on acetylcholinesterase Langmuir and Langmuir–Blodgett films studied by surface potential and atomic force microscopy

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Cited by 16 publications
(8 citation statements)
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“…As shown in Figure 3 , the enzyme-substrate complex formation is influenced by pH. ChE is sensitive in extremely low and extremely high pH, thus leading to the loss of its enzymatic function [ 48 ]. At low pH, high concentrations of protons disrupt the interaction of substrate toward the ChE due to the protonation of an imidazole group of histidine at the catalytic triad of the enzyme [ 49 , 50 ].…”
Section: Discussionmentioning
confidence: 99%
“…As shown in Figure 3 , the enzyme-substrate complex formation is influenced by pH. ChE is sensitive in extremely low and extremely high pH, thus leading to the loss of its enzymatic function [ 48 ]. At low pH, high concentrations of protons disrupt the interaction of substrate toward the ChE due to the protonation of an imidazole group of histidine at the catalytic triad of the enzyme [ 49 , 50 ].…”
Section: Discussionmentioning
confidence: 99%
“…For Langmuir films, the surface potential depends on the material type [8] and subphase pH [9], with the surface potential arising from the dipoles and/or surface charge on the film [6]. For LB films, the surface potential can also depend on the type of substrate [8,10], number of deposited layers [11], pH [9], and temperature [12].…”
Section: Introductionmentioning
confidence: 99%
“…For Langmuir films, the surface potential depends on the material type [8] and subphase pH [9], with the surface potential arising from the dipoles and/or surface charge on the film [6]. For LB films, the surface potential can also depend on the type of substrate [8,10], number of deposited layers [11], pH [9], and temperature [12]. For an uncharged Langmuir monolayer, the surface potential is attributed entirely to dipole contributions whereas in transferred LB monolayers contributions may arise from dipoles [13], impurities (for example, water entrapped in the film), transferred charges at interface metal/film [12], and induced interfacial dipoles [14].…”
Section: Introductionmentioning
confidence: 99%
“…The subphase was then replaced with 0.1 mM solution of HCl in water to attain pH = 4 and 10.0 mM solution of HCl in water to attain pH = 2. [27] Again, solutions of B1 and B2 in chloroform were spread at these pH values. pH measuring probe for KSV NIMA System (KN 0011) was attached through interface.…”
Section: Materials and Experimentsmentioning
confidence: 99%