Effect of Pesticide on Nitrate Reductase Activity in Trigonella Foenum i.e., Fenugreek

Abstract

Nitrate reductase (NR) is the key enzyme of nitrate assimilation subjected to inhibition by excessive use of pesticides. Now a day's pesticides are used commonly at a large scale on field. In the present study, the effect of pesticides on nitrate reductase activity has been studied by in vivo assay in Trigonella foenum-graecum. The four pesticides Confidor, Omite, Karathane, Chlorpyrifos+Cypermethrinwere used for study at various concentrations such as 25, 50,100 and 200 µl/100 ml. The application of pesticides showed decrease in activity of nitrate reductase enzyme. Out of these pesticides confidor affected the nitrate reductase activity maximum. The nitrate reductase activity in control plant was found to be 0.460U ± 0.005 which was reduced up to 0.192 ± 0.008U in presence of confidor. Even in the tissue specific assay it was seen that the leaves and stem of the plant, the NR activity was affected maximum than other parts. The use of pesticides lowers the NR activity of the Trigonella plants thereby affecting its nitrogen metabolism.

Evaluation of Oxidative Stress on Soybean (Glycine max L. Merrill) and Wheat (Triticum aestivum) Crops in Response to Chlorpyrifos

“…A result was well supported by Pankaj et al, (2015) also reported significant decrease in activity with increase in concentration of pesticide in Fenugreek.…”

Side Effects of Pesticides and Metabolites in Groundwater: Impact on Denitrification

“…This suggests that the impact of the tested compounds probably occurs rather on gene expression and/or enzymatic activity itself. Pankaj and Gore (2015) already showed that the activity of enzyme nitrate reductase of plants was inhibited by some phytopharmaceutical products (confidor, omite, and karathane) and their active substances (imidacloprid, propargite, and meptyldinocap). The work of Saez et al (2003) also suggested that some pesticides (methylparathion, atrazine, and simazine) affected the expression of the nitrate reductase activity of the bacterial strain P. denitrificans at a concentration of 10 mg/L.…”