Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep

Watanabe, Hitoshi; Saito, Ryo; Nakano, Tatsuya; Takahashi, Hitomi; Takahashi, Yu; Sumiyoshi, Keisuke; Sato, Kei; Chen, Xiangning; Okada, Naoya; Iwasaki, S.; Harjanti, Dian Wahyu; Sekiguchi, Natsumi; Sano, Hiroaki; Kitazawa, Haruki; Rose, Michael T.; Ohwada, Shyuichi; Watanabe, Kouichi; Aso, Hisashi

doi:10.1371/journal.pone.0088058

Cited by 34 publications

(23 citation statements)

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“…Interestingly, in an experiment conducted in wethers that were fasted for 24 h, i.v. treatment with serotonin, not 5-HTP, increased both glucose and insulin concentrations for 1 h post-treatment (Watanabe et al 2014). This indicates that serotonin may act differently depending on the stage of the energy balance of the animal.…”

Section: Discussionmentioning

confidence: 87%

“…I.v. administration of serotonin to wethers also increased NEFA concentrations 1 h post-treatment, but concentrations then returned to baseline (Watanabe et al 2014). This suggests that even though we administered a serotonin precursor and not serotonin itself in the present study, the effects on NEFA concentrations would not have been long-lasting either way.…”

Section: Discussionmentioning

confidence: 94%

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Increasing serotonin concentrations alter calcium and energy metabolism in dairy cows

Laporta

Moore

Weaver

et al. 2015

Journal of Endocrinology

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A 4!4 Latin square design in which varied doses (0, 0.5, 1.0, and 1.5 mg/kg) of 5-hydroxy-L-tryptophan (5-HTP, a serotonin precursor) were intravenously infused into late-lactation, non-pregnant Holstein dairy cows was used to determine the effects of serotonin on calcium and energy metabolism. Infusion periods lasted 4 days, with a 5-day washout between periods. Cows were infused at a constant rate for 1 h each day. Blood was collected pre-and 5, 10, 30, 60, 90, and 120 min post-infusion, urine was collected pre-and post-infusion, and milk was collected daily. All of the 5-HTP doses increased systemic serotonin as compared to the 0 mg/kg dose, and the 1.0 and 1.5 mg/kg doses increased circulating glucose and non-esterified fatty acids (NEFA) and decreased beta-hydroxybutyrate (bHBA) concentrations. Treatment of cows with either 1.0 or 1.5 mg/kg 5-HTP doses decreased urine calcium elimination, and the 1.5 mg/kg dose increased milk calcium concentrations. No differences were detected in the heart rates, respiration rates, or body temperatures of the cows; however, manure scores and defecation frequency were affected. Indeed, cows that received 5-HTP defecated more, and the consistency of their manure was softer. Treatment of late-lactation dairy cows with 5-HTP improved energy metabolism, decreased loss of calcium into urine, and increased calcium secretion into milk. Further research should target the effects of increasing serotonin during the transition period to determine any benefits for post-parturient calcium and glucose metabolism.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 94%

Increasing serotonin concentrations alter calcium and energy metabolism in dairy cows

Laporta

Moore

Weaver

et al. 2015

Journal of Endocrinology

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“…Recently, Weaver et al (2016) and Hernández-Castellano et al (2017) demonstrated that the infusion of the 5-HT precursor 5-hydroxytryptophan (5-HTP) during the last days before parturition improved blood calcium concentrations around parturition. Additionally, 5-HT has been shown to influence several metabolites related to glucose and lipid metabolism such as glucose, insulin, triglyceride, cholesterol, and fatty acid concentrations in mice (Sugimoto et al, 1990;Watanabe et al, 2010) and sheep (Watanabe et al, 2014). Moreover, 5-HT promotes the proliferation and activation of B cells in mice (Iken et al, 1995), and therefore the IgG concentration could also be affected by the increased 5-HT concentration in blood.…”

Section: Introductionmentioning

confidence: 99%

Endocrine and metabolic changes in transition dairy cows are affected by prepartum infusions of a serotonin precursor

Hernández-Castellano

Hernández

Sauerwein

et al. 2017

Journal of Dairy Science

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Serotonin (5-HT) has been shown to be involved in calcium homeostasis, modulating calcium concentration in blood. In addition, 5-HT participates in a variety of metabolic pathways, mainly through the modulation of glucose and lipid metabolism. The hypothesis of the present study was that the prepartum administration of 5-hydroxy-l-tryptophan (5-HTP), a 5-HT precursor, would affect endocrine systems related to calcium homeostasis, and interact with other endocrine and metabolic pathways during the transition period. In this study, 20 Holstein dairy cows were randomly assigned to 2 experimental groups. Both groups received a daily i.v. infusion of 1 L of either 0.9% NaCl (control group; n = 10) or 0.9% NaCl containing 1 mg of 5-HTP/kg of BW (5-HTP group, n = 10). Infusions started d 10 before estimated parturition date and ended the day of parturition, resulting in a minimum of 4 d of infusion (8.4 ± 0.7 d of infusion). Until parturition, blood samples were collected before the daily infusions, and postpartum daily until d 7, and on d 30. Plasma concentrations of parathyroid hormone (PTH) were transiently increased at parturition and on d 1 in control cows. In the 5-HTP group PTH remained unchanged. The concentration of pyridinoline (PYD), an established marker for calcium release from the bone to the bloodstream, increased on d 1 postpartum only in the 5-HTP group. In control cows, PYD concentrations did not change on d 1 postpartum. Melatonin concentrations were slightly but significantly increased in the 5-HTP group compared with the control group. Insulin concentrations decreased in both groups postpartum. Before parturition, leptin concentrations decreased in both groups and remained at this level until d 30 postpartum. Plasma IgG concentrations decreased in both groups on d -1 postpartum. Haptoglobin increased in both groups on d -1 and remained at this level until d 7 postpartum. No differences between groups were observed for insulin, glucagon, IgG, leptin, adiponectin, and haptoglobin concentrations. The results obtained in the present study evidenced that 5-HT is regulating calcium homeostasis independent of PTH. The lack of treatment effects on IgG and on other hormones and metabolites indicates that 5-HTP did not affect these other metabolic pathways and the IgG concentration during the transition period.

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“…Ruminants absorb little glucose and have no glucokinase activity in the liver, and nearly all of their glucose needs must be compensated by gluconeogenesis. Propionate is the principal source of carbon for glucose synthesis in liver, which meets 85%-90% of the body glucose requirements in sheep (Catunda et al, 2013;Watanabe et al, 2014). Alfalfa hay had a more effective role than sodium propionate on calf performance and rumen development (Beiranvand et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Nutritional value, performance, feeding behavior and serum biochemical profile of sheep fed with alfalfa hay replacing Bermuda grass ( Cynodon dactylon (L.) Pers.) hay

Silva

Véras

Carvalho

et al. 2017

Chil. j. agric. res.

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Grasses and legumes are two groups of plants physic and chemically different (amount of crude protein and fiber, mostly), very used in animal feed, whose differences can determine variation in intake, digestibility and animal behavior. We aimed to evaluate performance, feeding behavior and the serum biochemical profile of sheep. The treatments were four levels of substitution of Bermuda grass (Cynodon dactylon [L.] Pers.) hay for alfalfa (Medicago sativa L.) hay (0, 330, 660 and 1000 g kg -1 DM). The randomized block design was used being two blocks and four treatments with 10 replicates). There were no effects on the performance of animals. On the other hand, there was a positive linear effect (P < 0.05) for intake of DM, organic matter, crude protein (CP), total carbohydrates, and non-fibrous carbohydrates. However, the use of neutral detergent fiber (NDF) showed linear decrease with the increase in alfalfa hay (P < 0.05). CP and NDF decreased linearly (P < 0.05) with the replacement of Bermuda grass hay for alfalfa hay. There were decreasing linear effects (P < 0.05) when compared to the time of ingestion and chewing of feed, and increasing linear effects regarding total resting time. Estimated passage rate, density of particles and blood glucose showed a positive linear effect (P < 0.05), while weight of gastrointestinal contents decreased according to alfalfa levels (P < 0.05). The replacement of Bermuda grass hay by alfalfa hay to promote improvement in the performance is not recommended.Key words: Behavior, digestibility, intake, legumes, Medicago sativa. ABSTRACTNutritional value, performance, feeding behavior and serum biochemical profile of sheep fed with alfalfa hay replacing Bermuda grass (Cynodon dactylon (L.) Pers.) hay

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Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep

Cited by 34 publications

References 50 publications

Increasing serotonin concentrations alter calcium and energy metabolism in dairy cows

Increasing serotonin concentrations alter calcium and energy metabolism in dairy cows

Endocrine and metabolic changes in transition dairy cows are affected by prepartum infusions of a serotonin precursor

Nutritional value, performance, feeding behavior and serum biochemical profile of sheep fed with alfalfa hay replacing Bermuda grass ( Cynodon dactylon (L.) Pers.) hay

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