Effect of peripartum nutritional supplementation on postpartum fertility and blood biochemical and steroid hormone profile in crossbred cows

Abstract

Advanced pregnant healthy HF crossbred cows (n=20) of 2-4 parity were equally divided in to control (routine farm feeding-RFF) and treatment/nutrients supplementation (RFF + bypass fat @ 100-200 g/h/d and ASMM @ 50 g/h/d) groups and were studied from 2 wks prepartum to 8 wks postpartum for plasma profile of steroid hormones and metabolites on days -14, -3, 0, +3, +14, +28 and +42 as well as for puerperal events and postpartum fertility. The mean plasma progesterone values were maximum (>6 ng/ml) on day 14 prepartum, which declined significantly (p<0.01) on day 3 prepartum reached to the basal levels (<1 ng/ml) on the day of calving, remained basal till day 14, and thereafter showed a rising trend on days 28 and 42 postpartum. The oestradiol-17 values were at its peak on the day of calving (p<0.01), showed a rapid fall by day 3 postpartum and remained low till recrudesce of follicular activity around day 35 postpartum. However, there were no statistical differences between the two groups in either of the hormones, except on day 42 postpartum. The levels of cortisol and PGF 2  Metabolites (PGFM) were 3-8 times higher on the day of parturition as compared to values at day 14 pre-and postpartum, and declined further till day 42 postpartum reaching to prepartum levels. The plasma cholesterol gradually decreased as parturition approached and increased in postpartum days to reach the highest value (p<0.01) at day 42. The nutrients supplemented cows had significantly (p<0.01) higher plasma cortisol, PGFM and cholesterol values than the control cows around parturition. However, no such variation was noted in plasma protein profile. The period of uterine involution in control and supplemented groups was identical (31.97±1.82 and 30.27±1.41 days), yet the cows in treatment group resumed estrous cycle earlier (38.00±1.95 vs 42.32±4.14 days, p<0.05) and had shorter service period (85.22±7.17 vs 100.67±5.60 days) with improved pregnancy rate (80 vs 60 %) as compared to those in control group. Thus, the peripartum nutrient supplementation in crossbred cows was beneficial and had positive effect on the postpartum fertility and plasma cortisol, PGFM and cholesterol profile.

Effect of peripartum nutritional management on plasma profile of steroid hormones, metabolites, and postpartum fertility in buffaloes

“…This trend of peripartum plasma progesterone concentration corroborated well with the earlier reports in buffaloes [8-11] and cattle [3,12]. In this study, basal value of 0.2-0.8 ng/ml found on the day of calving was suggestive of complete luteolysis at parturition and corroborated with the findings of previous workers [13-16].…”

Impact of bypass fat and mineral supplementation peripartum on plasma profile of steroid hormones PGFM and postpartum fertility in Jaffarabadi buffaloes

“…Dietary supplementation of fat increases the circulating concentrations of cholesterol, which serves as a precursor for the synthesis of progesterone by ovarian luteal cells and also increases the lifespan of induced CL (Rahbar et al, 2014). The gradual rise in mean plasma progesterone level on day 45 and 60 postpartum in the present study is supported by the findings of previous workers (Dhami et al, 2017;Kalasariya et al, 2017), who also observed gradual rise in progesterone prior to first postpartum estrus in cattle and buffalo.…”

“…The present significant decreasing trend prepartum and increasing trend postpartum of plasma progesterone concentrations observed in buffaloes during transitional period corroborated with the earlier findings of Ullah et al (2010), Ashmawy (2015) and Kalasariya et al (2017) in buffaloes and Gowda et al (2015) and Dhami et al (2017) in cattle. In the present study, the basal value of progesterone found on the day of calving was suggestive of complete luteolysis at parturition and corroborated with the findings of Momongan et al (1990) and Kalasariya et al (2017).…”

“…Almost similar trends / patterns of plasma estradiol were noted with these treatments under major control and oral nutrients supplemented/treatment groups. The overall trend of prepartum and postpartum estradiol-17β profile found in animals under study coincided well with that reported by many of the earlier researchers in buffaloes (Arya and Madan, 2001;Dugwekar et al, 2008;Ashmawy, 2015 andKalasariya et al, 2017) and in cattle (Dhami et al, 2017).…”

Icariin impacts molecular changes in estrogen receptors but not other reproductive parameters in female mice

“…Female reproduction including ovary development is influenced by estrogen and progesterone hormones, the genes that regulate their production and the estrogen and progesterone receptors that mediate their action Kang et al (2012a) Naikoo et al (2016) and Dhami et al (2017). The purpose of the study was therefore, to determine the role of ICA in reproduction by assessing the reproductive index (RI), In Vitro Maturation (IVM) rates of oocytes seen as extrusion of polar body 1 (pb1), steroidogenesis of 17-estradiol (17-E 2 ) and progesterone (P 4 ), gene expression of steroidogenic (Star, Cyp19a1 and Hsd17b1) genes, important in estrogen synthesis and protein expression of estrogen receptors (ER) ,  and progesterone receptor A (PRA).…”