Effect of periodic temperature shock on nitrogen removal performance and microbial community structure in plug-flow microaerobic sludge blanket

Ma, Xiao; Bo, Zhao; Zhang, Xiao; Xie, Fei; Cui, Ying; Li, Hui; Yue, Xiuping

doi:10.1016/j.chemosphere.2019.124934

Cited by 16 publications

(4 citation statements)

References 47 publications

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“…Interestingly, in opposite to this study and our previous work, Ma et al [33] studied the effect of periodic elevation of temperature, showing increased nitrogen removal efficiency and abundance of functional bacterial species including AOB, NOB and anammox.…”

Section: Applicability To Main-stream Nitrogen Removalcontrasting

confidence: 99%

Adaptation of flocculent anammox culture to low temperature by cold shock: long-term response of the microbial population

Kouba

Gerlein

Benáková

et al. 2021

Environmental Technology

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Partial nitritation-anammox (PN/A) process will substantially reduce the costs for the removal of nitrogen in the mainstream of municipal sewage. However, one of the mainstream PN/A challenges is to reduce the time necessary for the adaptation of anammox bacteria to lower temperatures in mild climates. In this study, we exposed anammox flocculent culture to cold shocks [35°C → 5°C (8 h) → 15°C] and evaluated long-term cold shock response. Over a postshock period of 40 d at 15°C, the nitrogen removal rates in the shocked culture were significantly higher compared to control, with maximum rates up to 0.082 and 0.033 kg-N/kg-VSS/d or 0.164 and 0.076 kg-N/m 3 /d, for shocked culture and control, respectively. In the corresponding semi-batch cycles, the shocked culture was on average 136 ± 101% more active than the control, due to the negative effect of cold shock on side populations and more active anammox cells. Per FISH, Ca. Brocadia anammoxidans and Ca. Scalindua survived the shock and remained present throughout. Thus, both anammox microorganisms seem to respond favourably to cold shocks. In sum, we provide further evidence that cold shocks accelerate the adaptation of anammox to the mainstream of municipal WWTPs. Further, for the first time, we report the long-term adaptive response of anammox to cold shocks.

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Section: Applicability To Main-stream Nitrogen Removalcontrasting

confidence: 99%

Adaptation of flocculent anammox culture to low temperature by cold shock: long-term response of the microbial population

Kouba

Gerlein

Benáková

et al. 2021

Environmental Technology

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“…The activity and quantity of enriched phosphorus accumulating bacteria showed a decreasing trend at increasing temperatures, but when the temperature decreased, the activity of phosphorus accumulating bacteria Acinetobacter enhanced its phosphorus removal effect (Fang et al, 2011). Ma and coworkers (Ma et al, 2020) assessed induced temperature periodic changes. They found that the activity a50nd relative abundance of ammonia oxidizing bacteria, nitrite oxidizing bacteria, and anammox bacteria, were dominated by Candidatus Brocadia at low temperatures, which changed following periodic temperature shocks.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the community-structure of A2O processes under different temperature conditions in plateau areas

Yongli

Huang

et al. 2020

Preprint

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Background: In this study, laboratory-scale A2O wastewater treatment was performed in Linzhi at an altitude of 3000 meters. Water temperatures were maintained at four operating conditions (25°C, 20°C, 15°C, and 10°C). Sludge in the aerobic tank was assessed by 16SrRNA sequencing and composition analysis. The Phylum, Class, Order, Family, Genus, and Species of the sludge were also confirmed.Results: The bacteria within the sludge showed significant differences at varying temperatures (P<0.05). A significant correlation between the bacteria numbers in anaerobic tanks and anoxic tanks also occurred. Indicators of community richness, community uniformity, community diversity and other areas showed differences. Significant differences in bacteria abundance were also observed and differed to those of previously reported superior community categories and proportions. T-tests were used to identify temperature-sensitive community at each level. Correlation analysis of environmental factors and colony structure further confirmed the association between temperature and colony structure (P<0.05). The removal rates of TP, TN, NH3-N, and COD were all affected by the sample community structure. The variety of colony structure include nitrifying bacteria, denitrifying bacteria, phosphorus accumulating bacteria and other bacterial differed, but their proportions were relatively low. Heatmaps were used to identify species sensitive to temperature, TP, TN, NH3-N, COD at the species level.Conclusions: Amongst the common dominant bacteria at the species level, Bacteroidetes_bacterium_OLB10 was significantly related to temperature. Other common dominant bacteria that were related to temperature metagenome_g__Gordonia, unclassified_f__Burkholderiaceae, uncultured_bacterium_g__norank_f__Saprospiraceae.

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“…DO, T and ORP correlate negatively with the proportional removal of nitrate, but positively with those of TAN, COD, and TN. These correlations suggest that higher DO concentrations could provide more electron acceptors for heterotrophs and nitrifiers to oxidize organics and TAN, respectively (Machat et al 2019;Jiang et al 2020), and higher temperature could intensify their activity (Ma et al 2020). These conditions would produce more nitrate facilitating and stronger denitrification processes in the reactors.…”

Section: Rda Analysis Between Performance and Effluent Characteristicsmentioning

confidence: 94%

Response of simultaneous nitrification-denitrification to DO increments in continuously aerated biofilm reactors for aquaculture wastewater treatment

Zhang

Chen

Yuan

et al. 2021

Water Practice and Technology

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Intensive aquaculture usually produces large volumes of nutrient-rich wastewater, which is essential to treat to avoid eutrophication. This study aimed to evaluate the performance of five, continuously aerated, biofilm reactors treating simulated, high-strength, aquaculture wastewater under different dissolved oxygen (DO) levels, and the effects of DO increments on simultaneous nitrification-denitrification (SND). Continuous aeration was beneficial to complete nitrification. Total inorganic nitrogen (TIN), principally ammonium, was mainly removed by SND. The SND rate response to different DO levels was fitted well by the power function of y = 54.81 + 371.58/(1 − 0.16*x)^(−1/0.24) (R2 = 0.897, P = 0.000). When the TIN was removed completely, the optimal SND rate was defined and corresponded to a value of 121.8%. Accordingly, the optimal DO concentration was calculated as 2.10 mg/L, close to the actual level of 1.83 mg/L, at which the highest proportional removals of total nitrogen (58.0%) and TIN (57.3%) were obtained. Phosphorus was also removed by denitrifying polyphosphate-accumulating organisms.

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Effect of periodic temperature shock on nitrogen removal performance and microbial community structure in plug-flow microaerobic sludge blanket

Cited by 16 publications

References 47 publications

Adaptation of flocculent anammox culture to low temperature by cold shock: long-term response of the microbial population

Adaptation of flocculent anammox culture to low temperature by cold shock: long-term response of the microbial population

Characteristics of the community-structure of A2O processes under different temperature conditions in plateau areas

Response of simultaneous nitrification-denitrification to DO increments in continuously aerated biofilm reactors for aquaculture wastewater treatment

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