Effect of Perfusion during Ischemia on Skeletal Muscle

Tsuchida, Toru; Kato, Teiji; Yamaga, Makio; Ikebe, Kenshiro; Oniki, Yasunari; Irie, Hiroki; Takagi, Katsumasa

doi:10.1006/jsre.2001.6278

Cited by 14 publications

(7 citation statements)

References 9 publications

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“…In the past, different approaches were conducted to optimize tissue conservation during ischaemia . Currently, in reconstructive surgery flaps are mostly treated with a single flush of heparinized crystalloid solution after harvesting, followed by cold storage until their reconnection to blood circulation.…”

Section: Discussionmentioning

confidence: 99%

Ischaemia‐related cell damage in extracorporeal preserved tissue – new findings with a novel perfusion model

Taeger

Müller‐Seubert

Horch

et al. 2014

J Cellular Molecular Medi

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Tissue undergoing free transfer in transplant or reconstructive surgery always is at high risk of ischaemia-related cell damage. This study aims at assessing different procedures using an extracorporeal perfusion and oxygenation system to investigate the expression of hypoxia inducible factor (HIF)-1-α as marker for hypoxia and of the pro-apoptotic protein Caspase-3 in skeletal muscle to elucidate potential improvements in tissue conservation. Twenty-four porcine rectus abdominis muscles were assigned to five different groups and examined after they had been extracorporeally preserved for 60 min. time. Group I was left untreated (control), group II was perfused with a cardioplegic solution, group III was flushed with 10 ml of a cardioplegic solution and then left untreated. Group IV and V were perfused and oxygenated with either an isotone crystalloid solution or a cardioplegic solution. Among others, immunohistochemistry (Caspase-3 and HIF-1-α) of muscle samples was performed. Furthermore, oxygen partial pressure in the perfusate at the arterial and venous branch was measured. Expression of Caspase-3 after 60 min. was reduced in all groups compared to the control group. Furthermore, all groups (except group III) expressed less HIF-1-α than the control group. Oxygenation leads to higher oxygen levels at the venous branch compared to groups without oxygenation. Using an extracorporeal perfusion and oxygenation system cell damage could be reduced as indicated by stabilized expressions of Caspase-3 and HIF-1-α for 60 min. of tissue preservation. Complete depletion of oxygen at the venous branch can be prevented by oxygenation of the perfusate with ambient air.

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Section: Discussionmentioning

confidence: 99%

Ischaemia‐related cell damage in extracorporeal preserved tissue – new findings with a novel perfusion model

Taeger

Müller‐Seubert

Horch

et al. 2014

J Cellular Molecular Medi

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“…The use of hemoglobin was thereafter abandoned and extremities were washed out [33,34], stored [35][36][37], perfused intermittently [34,38] or continuously [38][39][40] with fluids of different compositions, including physiological saline [41], Ringer Lactate [39], compositions using mannitol [42], and the standard organ preservation solutions University of Wisconsin [34,35,40,[43][44][45] and Euro Collins [33,39,43,45]. To increase the oxygenation of the perfused extremity, artificial oxygen carriers like Fluosol [36,38,46] and Fluorocarbon [38] have been used and one study even investigated the effect of hyperbaric oxygen chambers [47].…”

Section: Discussionmentioning

confidence: 99%

Preservation of Amputated Extremities by Extracorporeal Blood Perfusion; a Feasibility Study in a Porcine Model

Constantinescu

Knall

et al. 2011

Journal of Surgical Research

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“…This finding may suggest that CO therapy provides some degree of metabolic rescue. ATP levels have been previously shown to be proportional to muscle viability(19, 20). Work studying myocardial IR reported a marked improvement in myocardial energetics associated with inhaled CO (1).…”

Section: Discussionmentioning

confidence: 99%

Inhalation of carbon monoxide reduces skeletal muscle injury after hind limb ischemia-reperfusion injury in mice

Patel

Albadawi

Steudel

et al. 2012

The American Journal of Surgery

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Introduction The purpose of this study was to determine if inhaled carbon monoxide (CO) can ameliorate skeletal muscle injury, modulate endogenous heme oxygenase-1 (HO) expression, improve indices of tissue integrity and inflammation following hind limb ischemia reperfusion(IR). Methods C57BL6 mice inhaling CO (250ppm) or room air were subjected to 1.5 hrs of ischemia followed by limb reperfusion for either 3 or 6 hours (total treatment time of 4.5 or 7.5 hrs). After the initial period of reperfusion, all mice breathed only room air until 24 hours after the onset of ischemia. Mice were sacrificed at either the end of CO treatment or at 24 hours reperfusion. Skeletal muscle was subjected to histologic and biochemical analysis. Results CO treatment for 7.5 hours protected skeletal muscle from histologic and structural evidence of skeletal muscle injury. Serum and tissue cytokines were significantly reduced (p<0.05) in mice treated with CO for 7.5 hours. Tubulin, Heme Oxygenase, and ATP levels were higher in CO treated mice. Conclusions Inhaled CO protected muscle from structural injury and energy depletion following IR.

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Effect of Perfusion during Ischemia on Skeletal Muscle

Cited by 14 publications

References 9 publications

Ischaemia‐related cell damage in extracorporeal preserved tissue – new findings with a novel perfusion model

Ischaemia‐related cell damage in extracorporeal preserved tissue – new findings with a novel perfusion model

Preservation of Amputated Extremities by Extracorporeal Blood Perfusion; a Feasibility Study in a Porcine Model

Inhalation of carbon monoxide reduces skeletal muscle injury after hind limb ischemia-reperfusion injury in mice

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