Effect of Peptides from Plasma of Patients with Coronary Artery Disease on the Vascular Endothelial Cells

Kozyk, Marko; Strubchevska, Kateryna; Marynenko, Tetiana; Zlatska, Alona; Halenova, Tetiana; Raksha, Nataliia; Savchuk, Olexiy; Falalyeyeva, Tetyana; Kovalchuk, Oleksandr; Ostapchenko, L.

doi:10.3390/medicina59020238

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…Post COVID-19 condition can affect multiple organ systems including the respiratory, gastrointestinal, neurological, or cardiovascular. 23 We investigated the key anticoagulation parameters of donor plasma during the post-COVID-19 period and established that the changes in the concentration and activities of ATIII, PC, and TM depended on the titers of anti-SARS-CoV-2 IgG. This is a potential reason for prolonged thrombotic complications after recovery.…”

Section: Discussionmentioning

confidence: 99%

Coagulation Parameters in Post-Covid-19 Condition in Relation to Various Titers of Anti-SARS-CoV-2 IgG in Blood Plasma

Strubchevska,

Rachkovska,

Krenytska

et al. 2023

IJGM

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Background and Objectives Post-COVID-19 condition is thought to affect 10–20% of people at least 3 months after a diagnosis of COVID-19 and two months of symptoms. Post-COVID-19 condition presents itself with many clinical effects with varying degrees of severity ranging from a mild cough to a life-threatening coagulopathy. Our study aimed to identify a relationship between the titers of anti-SARS-CoV-2 IgG and anticoagulation parameters: antithrombin III (ATIII), protein C (PC) and thrombomodulin (TM). Materials and Methods Blood plasma was collected from healthy donors aged 25–45 who had recovered from COVID-19 3–6 months ago and their titers of anti-SARS-CoV-2 IgG and ATIII, PC, and TM were measured. Results We found that concentrations and activities of key anticoagulation parameters (ATIII, PC, and TM) measured in donor plasma during the post-COVID-19 varied in relation to the titers of anti-SARS-CoV-2 IgG. Conclusion While we identified a dysfunction of anticoagulation parameters in patients with post-COVID-19, we aim to explore the subpopulation antibody IgG fraction directly using in vivo and in vitro experiments with the possibility to contribute to the development of treatment options for post-COVID-19 conditions.

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Section: Discussionmentioning

confidence: 99%

Coagulation Parameters in Post-Covid-19 Condition in Relation to Various Titers of Anti-SARS-CoV-2 IgG in Blood Plasma

Strubchevska,

Rachkovska,

Krenytska

et al. 2023

IJGM

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“…Determination of plasma levels of hemostasis factors, such as prothrombin, plasminogen, tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), protein C (PC), soluble thrombomodulin (TM) was performed by means of enzyme-linked immunosorbent assay (ELISA) [19,21]. Briefly, plasma samples were diluted 1:100 with 0.05 M Tris-HCl buffer (pH 7.4) and added into the wells of ELISA plates.…”

Section: Methodsmentioning

confidence: 99%

Coagulation and Anticoagulation Parameters in Multiple Sclerosis Patients With and Without Covid-19

Halenova,

Raksha,

Vovk

et al. 2024

Clin. and prev. med.

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The aim. To investigate plasma levels of main coagulation and fibrinolytic factors in MS patients with and without COVID-19 history. Materials and methods. A total of 127 participants were enrolled in this study, including 97 MS patients and 30 healthy controls (HC). Patients with MS were divided into two groups: MS+Covid group (n=41) – patients with MS, who had a laboratory-verified diagnosis of COVID-19 in the past 3-6-month period and MS group (n=56) – patients with MS, who did not suffer from COVID-19 previously. Determination of plasma levels of prothrombin, plasminogen, tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), protein C (PC), soluble thrombomodulin (TM) was performed by means of enzyme-linked immunosorbent assay. Spectrophotometric techniques were used to determine concentrations of fibrinogen, soluble fibrin monomeric complexes (SFMC) as well as plasminogen activity and inhibitory potential of α-2-antiplasmin. Results. The MS group was characterized by elevated levels of plasma prothrombin, fibrinogen, D-dimer, SFMC, soluble TM compared to HC, while PC concentration did not differ between MS and HC groups. Plasma plasminogen level as well as plasma level of the potential plasmin activity were significantly decreased in MS patients compared to HC group. The plasma tPA level was significantly reduced while plasma PAI-I level was significantly increased in MS patients compared to HC. Patients of MS group had an increased level of plasma α-2-antiplasmin activity compared with HC group. To note, most of studied parameters did not differ between two MS groups, except protein C, soluble thrombomodulin levels and plasma α-2-antiplasmin activity. Conclusions. The results of our study showed that MS patients have got altered hemostasis parameters; however, further study is necessary to find out the relationship between particular components of coagulation and fibrinolytic systems and pathophysiology of MS. Additionally, our findings demonstrated that a SARS-CoV-2 infection had a limited effect on hemostasis parameters in MS patients, causing changes in only a few parameters, including thrombomodulin and protein C levels as well as α-2-antiplasmin activity.

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“…Plasma plasminogen levels were determined by enzyme-linked immunosorbent assay (ELISA) according to ( 18 ). Before use, blood plasma was diluted to 10 μg/mL with 50 mmoL/L Tris–HCl buffer (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of proteolytic activity and serine proteases distribution in plasma from patients with bladder cancer

Synelnyk,

Vovk,

Halenova

et al. 2023

Front. Med.

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BackgroundBladder cancer (BC) is an aggressive disease with a poor prognosis. A bladder tumor, like other malignant neoplasms, is characterized by the presence of both cancer cells and stromal cells which secrete cytokines, chemokines, growth factors, and proteolytic enzymes. One such class of proteolytic enzymes are serine proteases, which take part in the tumor microenvironment formation via supporting and contributing to tumor progression. This study aims to evaluate the proteolytic activity and serine protease contribution in plasma from BC patients.MethodsThe research involved patients of Alexandrovsky city clinical hospital aged 52–76 with transitional cell carcinoma of the bladder. All examined patients were divided into five groups: the control group included conditionally healthy donors, while other patients were grouped according to their tumor stage (I, II, III and IV). Plasma plasminogen levels were determined by enzyme-linked immunosorbent assay, and the potential activity was measured by chromogenic plasminogen assay. Serine proteases fractions were obtained by the affinity chromatography method, and enzyme concentration in the selected fractions were determined by the Bradford method. Serine proteases distribution was investigated by electrophoresis in a polyacrylamide gel.ResultsIt was determined that the concentration, potential activity of plasminogen, and the total amount of serine proteases in plasma from BC patients were greater than the values of the corresponding indicators in healthy donors. This could be one of the factors contributing to increased proteolysis seen in the process of carcinogenesis. Plasminogen concentration in BC patients with stage IV disease; however, displayed a tendency to be reduced compared to earlier stages, and the potential activity of plasminogen was significantly lower in patients with stages III – IV BC. Futhermore, a tumor stage specific gradual decline in the serine protease plasma content was shown. The results of electrophoretic analysis established a significant diminishment in the percentage of high molecular weight components (under non-reducing conditions) and their complete disappearance (under reducing conditions) in plasma serine protease fractions from BC patients. A decline in the percentage of heavy and light plasmin chains in BC patients was also observed. Additionally, a rise in the degraded forms of plasminogen/plasmin content was seen in BC samples, as well as the presence of fractions corresponding to trypsin and NE (under non-reducing conditions) that were absent in the control samples.ConclusionThe results indicate significant changes in the proteolytic activity of plasma, from BC patients when compared to healthy controls, which is accompanied by alterations in serine protease distribution caused by tumor microenvironment pecularlities at the different stages of oncopathology.

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Effect of Peptides from Plasma of Patients with Coronary Artery Disease on the Vascular Endothelial Cells

Cited by 7 publications

References 34 publications

Coagulation Parameters in Post-Covid-19 Condition in Relation to Various Titers of Anti-SARS-CoV-2 IgG in Blood Plasma

Coagulation Parameters in Post-Covid-19 Condition in Relation to Various Titers of Anti-SARS-CoV-2 IgG in Blood Plasma

Coagulation and Anticoagulation Parameters in Multiple Sclerosis Patients With and Without Covid-19

Evaluation of proteolytic activity and serine proteases distribution in plasma from patients with bladder cancer

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