Effect of Peptichemio upon Localization of Injected Radiolabelled Lymphocytes in the Lymphatic Organs of Antigen - Stimulated Mice

Caruso, Calogero; Benvegna, Salvatore; Palmeri, Patrizia; Cillari, E.

doi:10.1177/030089167806400103

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Preparations Of Lymph Node Cells1

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1981

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“…Cell suspensions were prepared as previously described [11]; peripheral lymph nodes were re moved, dissociated in ice-cold medium 199 (Gibco, New York, N.Y.) with 10% fetal calf serum (FCS; Gibco). Red cells were lysed by brief expo sure to hypotonic ammonium chloride in Tris buffer [8] and washed cells were suspended to the desired concentration of viable cells, as judged by a dye exclusion test (0.05% trypan blue).…”

Section: Preparations Of Lymph Node Cellsmentioning

confidence: 99%

Depression of Contact Sensitivity to Oxazolone by the Paramyxovirus of Newcastle Disease

Caruso¹,

Palmeri²,

Dieli³

et al. 1981

Int Arch Allergy Immunol

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The paramyxovirus of Newcastle disease (NDV) impairs the contact sensitivity to oxazolone in CBA/J mice: in vitro treatment with the infectious virus inhibits the passive transfer of contact sensitivity. Experiments performed with three different virus preparations demonstrate that only the infectious virus is able to inhibit the hypersensitivity reaction while treatment of virus by heat or ultraviolet irradiation wipes out its inhibitory activity. These results suggest that I-NDV depresses the response to oxazolone by its action on T immunoblasts which mediate the response to contactant.

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Section: Preparations Of Lymph Node Cellsmentioning

confidence: 99%