Effect of pentoxifylline on superoxide anion production by human sperm

Gavella, Mirjana; Lipovac, Vaskresenija; Marotti, Tatjana

doi:10.1111/j.1365-2605.1991.tb01099.x

Cited by 69 publications

(31 citation statements)

References 18 publications

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“…Gavella et al 71 and Gavella and Lipovac 72 found that superoxide anion production was reduced by the addition of 10 mmol l 21 of pentoxifylline, as did McKinney et al 73 and Okada et al 74 in their respective studies, albeit with different doses. It should also be stressed that in McKinney's study, 73 reduced lipid peroxidation was observed after the addition of pentoxifylline (dosages from 3.6 to 7.2 mmol l 21 ), and that in Okada's study 74 doses from 1 to 10 mmol l 21 led to preserved motility, due to reduced loss of curvilinear velocity and beat-cross frequency.…”

Section: Pentoxyfillinementioning

confidence: 77%

“…It should also be stressed that in McKinney's study, 73 reduced lipid peroxidation was observed after the addition of pentoxifylline (dosages from 3.6 to 7.2 mmol l 21 ), and that in Okada's study 74 doses from 1 to 10 mmol l 21 led to preserved motility, due to reduced loss of curvilinear velocity and beat-cross frequency. [71][72][73][74] However, in 1998 Twigg et al 19 found no improvement in lipid peroxidation after the addition of 3.6 mmol l 21 of pentoxifylline.…”

Section: Pentoxyfillinementioning

confidence: 99%

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The role of antioxidant therapy in the treatment of male infertility: an overview

Lombardo¹,

Sansone²,

Romanelli³

et al. 2011

Asian J Androl

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In recent years, many studies have focused on the effect of oxidative stress, reactive oxygen species (ROS) and antioxidants on the male eproductive system. Under physiological conditions, sperm produces small amounts of ROS, which are needed for fertilisation, acrosome reaction and capacitation. However, if an increased production of ROS is not associated with a similar increase in scavenging systems, peroxidative damage of the sperm plasma membrane and loss of DNA integrity typically occur, which leads to cell death and reduced fertility. Furthermore, since there is no linear correlation between sperm quality and pregnancy rates, an improvement in semen parameters should not be the sole outcome considered in studies of antioxidant therapies. A definitive conclusion regarding the benefit of these therapies is difficult to obtain, as most of the previous studies lacked control groups, considered different antioxidants in different combinations and doses, or did not evaluate pregnancy rates in previously infertile couples. Even if beneficial effects were reported in a few cases of male infertility, more multicentre, double-blind studies performed with the same criteria are necessary for an increased understanding of the effects of various antioxidants on fertility.

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Section: Pentoxyfillinementioning

confidence: 77%

Section: Pentoxyfillinementioning

confidence: 99%

The role of antioxidant therapy in the treatment of male infertility: an overview

Lombardo¹,

Sansone²,

Romanelli³

et al. 2011

Asian J Androl

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show abstract

“…The improvement of fertilisation may be caused by the antioxidant effect of pentoxifylline, as suggested by Gavella et al (1991). Moreover, Numabe et al (2001) found an increase in fertilisation, monospermy, cleavage and blastocyst rates after treating bull spermatozoa with pentoxifylline.…”

Section: Discussionmentioning

confidence: 90%

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Gil¹,

Hernández²,

Roca³

et al. 2010

Reproduction

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This study evaluated whether pentoxifylline added to freezing and thawing extenders influenced the function of boar spermatozoa. In Experiment 1, pooled ejaculated sperm-rich fractions were frozen in 0.5 ml straws after dilution in extender supplemented with pentoxifylline to a final concentration of 0, 2, 4, 8, 16 or 32 mM. The addition of 4, 8, 16 and 32 mM pentoxifylline to the freezing extender significantly decreased the progressive and total motility of spermatozoa. The percentage of viable spermatozoa with intact acrosomes as well as the penetration rate and the efficiency of fertilisation were significantly lower in pentoxifylline-treated groups compared with the untreated control. In Experiment 2, a pool of three straws with 'good' post-thaw sperm quality parameters and another three straws with 'poor' sperm quality were diluted in extender with 0, 1, 2, 4, 8, 16 or 32 mM pentoxifylline. Post-thaw samples with both 'good' and 'poor' sperm quality with 0, 2, 4, 8 and 16 mM were used to assess IVF parameters. The addition of pentoxifylline to post-thaw extender did not improve the post-thaw motility or viability of spermatozoa compared with the control. The in vitro penetration was higher (P!0.05) than the control for oocytes fertilised with spermatozoa that were thawed and incubated in extender with 4, 8 and 16 mM pentoxifylline. However, no differences were observed in the efficiency of fertilisation. We conclude that pentoxifylline, as a supplement added to the freezing extender, has a deleterious effect and that it does not improve the survival or in vitro fertilising efficiency of frozen-thawed boar spermatozoa when added after thawing.

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“…4,9 Recently, studies have focused on assessing the effectiveness of in vivo and in vitro supplementation with different antioxidants in an attempt to protect spermatozoa exposed to oxidative stress. [10][11][12][13][14] A beneficial effect of the in vitro supplementation of different natural or chemically synthesized antioxidant compounds on human spermatozoa has been observed; however, their effect has also been shown to decrease at inadequate concentrations and, even worse, to behave as pro-oxidants. 11,12,15,16 Herein, we discuss the protective role of exogenous gangliosides against ROS-induced changes based on our studies of human spermatozoa, leukocytes and cell-free systems.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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This article summarizes the available evidence on the efficacy of gangliosides to reduce the degree of reactive oxygen species (ROS)-mediated damage. The antioxidative efficacy of exogenous gangliosides in protecting different cells encouraged us to examine their ability to protect human spermatozoa. Gangliosides are sialic acid-containing glycosphingolipids with strong amphiphilic character due to the bulky headgroup made of several sugar rings with sialic acid residues and the double-tailed hydrophobic lipid moiety. The amphiphilicity of gangliosides allows them to exist as micelles in aqueous media when they are present at a concentration above their critical micellar concentration. The protective effect of ganglioside micelles on spermatozoa is believed to stem from their ability to scavenge free radicals and prevent their damaging effects. In our study, we particularly focused our attention on the protective effect of ganglioside micelles on DNA in human spermatozoa exposed to cryopreservation. The results indicate that ganglioside micelles can modulate the hydrophobic properties of the sperm membrane to increase tolerance to DNA fragmentation, thus protecting the DNA from cryopreservation-induced damage. Further actions of ganglioside micelles, which were documented by biochemical and biophysical studies, included (i) the modulation of superoxide anion generation by increasing the diffusion barrier for membrane events responsible for signal translocation to the interior of the cell; (ii) the inhibition of iron-catalysed hydroxyl radical formation due to the iron chelation potential of gangliosides; and (iii) inhibition of hydrogen peroxide diffusion across the sperm membrane.

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Effect of pentoxifylline on superoxide anion production by human sperm

Cited by 69 publications

References 18 publications

The role of antioxidant therapy in the treatment of male infertility: an overview

The role of antioxidant therapy in the treatment of male infertility: an overview

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

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