Effect of penconazole and drought stress on the essential oil composition and gene expression of Mentha pulegium L. (Lamiaceae) at flowering stage

Hassanpour, Halimeh; Khavari-Nejad, Ramazan Ali; Niknam, Vahid; Razavi, Khadijeh; Najafi, Farzaneh

doi:10.1007/s11738-014-1492-1

Cited by 33 publications

(19 citation statements)

References 23 publications

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“…An increase in carvacrol content in Satureja hortensis L. under limited water stress has been already reported, while terpinene content decreased under limited and intense water stress in this plant 40 . However, environmental stresses might affect the EO and its constituents through the influence on the transcription of the genes and activity of the enzymes involved in their biosynthesis 18,41 .…”

Section: Discussionmentioning

confidence: 99%

Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L.

Rezaie

Mandoulakani

Fattahi

2020

Sci Rep

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environmental stresses might alter the activity of antioxidant defense system and both quantity and quality of the essential oil constituents in aromatic plants. in the current study, a greenhouse experiment was designed to assess the influence of cold stress on total phenolic (TPC) and flavonoid contents (TFC), DPPH radical scavenging, antioxidant and phenylalanine ammonia-lyase (PAL) enzymes activity and content of phenylpropanoid compounds in Ocimum basilicum L. the genes expression levels of chavicol O-methyl transferase (CVOMT), cinnamate 4-hydroxylase (C4H), eugenol synthase 1 (EGS1) and eugenol O-methyl transferase (EOMT) were also investigated. Results revealed the highest TPC, TFC and DPPH at 4 °C for 12 h. Positive significant correlation was observed between TFC and DPPH, as well as TPC and PAL enzyme activity. The highest activity of superoxide dismutase and guaiacol peroxidase was recorded in 4 °C for 48 h, while this treatment caused the highest reduction in the activities of ascorbate peroxidase and catalase. In plants exposed to 10 °C for 48 h, the contents of methyleugenol and methylchavicol was positively associated with the expression levels of EGS1 and EOMT. A positive correlation was also found between C4H expression and eugenol, methyleugenol and methylchavicol contents under 4 °C for 12 h.Ocimum genus, belonging to family Lamiaceae, is a source of economically valuable aromatic oils with medicinal properties. This genus possesses an extensive intra-and inter-specific genetic diversification including 65 to more than 150 species spread throughout the world 1 . Ocimum sanctum and O. basilicum (sweet basil), two high priority species in this genus, are extensively used for their pharmaceutical and industrial importance. O. basilicum L. (2n = 4x = 48), originally from India, Iran and Afghanistan contains multiple terpenoid and phenylpropanoid compounds which are used in medicinal and perfume industries 2 . This compounds mostly produces in glandular trichomes on the leaf surface 3 . The most important compounds related to fragrance property are linalool, 1,8 cineole, methylchavicol (estragole) and eugenol. Basil essential oil (EO) has anticancer and hypoglycemic tuberculosis properties 4,5 . Also cytotoxic property of ethanolic extract of basil containing eugenol has been reported on human laryngeal cancer cells 5 . Terpenoids and phenylpropanoids such as eugenol, methylchavicol, methyleugenol and cinnamate are the main constitutes of basil EO 4 . The major phenylpropanoid components of basil are methylchavicol and methyleugenol. These compounds are extensively used in perfumes, aroma therapy, food and medicinal industries. Methylchavicol stimulates liver regeneration, shows hypothermic and DNA binding and spasmolytic activities. Phenylpropanoid compounds is produced through shikimate pathway. Phenylalanine ammonia-lyase (PAL), involved in the initial step of phenylpropanoid metabolism, deaminates phenylalanine to generate trans-cinnamic acid and ammonia 3 . Cinnamate 4-hydroxylase (C4H), a cyt...

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Section: Discussionmentioning

confidence: 99%

Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L.

Rezaie

Mandoulakani

Fattahi

2020

Sci Rep

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“…Deamination: The essential oils were analyzed through GC/MS, following the method described by Hassanpour et al [30]. Seeds or sprouts were air-dried, then 10 g of the dried sample was used for the extraction of essential oils.…”

Section: Essential Oil Analysismentioning

confidence: 99%

Laser Light Treatment of Seeds for Improving the Biomass Photosynthesis, Chemical Composition and Biological Activities of Lemongrass Sprouts

et al. 2021

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Compared to seeds and mature plants, sprouts are well characterized based on their nutritive values and biological properties. Moreover, laser light application is known to be a promising approach to improving plant growth, photosynthesis, and nutraceutical values. However, no studies have investigated the phytochemicals and biological activity of lemongrass (Cymbopogon proximus (Hochst. ex A.Rich.) Chiov.) sprouts or the further improvement of their quality by applying laser light treatment. We carried out a preliminary experiment for the optimization of laser treatment conditions, finding that a helium neon (He–Ne) laser at 632 nm and 5 mW for 5 min provided the most favorable conditions. We then investigated fresh weight, photosynthetic reactions, and primary and secondary metabolites, including sugars, amino acids, organic acids, essential oils, and phenolic compounds. Moreover, we studied the effect of laser light-induced changes in chemical compositions on the antioxidant, anti-diabetic, and anti-cholesterol activities of Cymbopogon proximus sprouts grown from laser-treated seeds. Laser light treatment increased the photosynthesis and respiration and hence the fresh weight of Cymbopogon proximus sprouts. Overall, sprouting increased most bioactive primary and secondary metabolites as compared to seeds. Increased photosynthesis by laser light improved carbon allocation and raised non-structural carbohydrates, which in turn led to improved synthesis of amino acids, organic acids, and essential oils, as well as phenolic and flavonoid compounds. As a result, laser light significantly improved the antioxidant capacity in terms of increasing the levels of ferric reducing antioxidant power (FRAP) (from 9.5 to 21 µmole trolox/g fresh weight (FW)), oxygen radical absorbance (ORAC) (from 400 to 1100 µmole trolox/100 g FW), and DPPH (from 5% to 25% of inhibation) and enhanced the hypocholesterolemic and antidiabetic activity through increasing the percentage of cholesterol micellar solubility (CMS) inhibition (from 42% to 62%) and glycemic index (from 33 to 17 µmole/g) over sprouts and seeds. In conclusion, the synergism of seed laser treatment and sprouting induced the health-promoting bioactive compounds in Cymbopogon proximus as compared to seeds, which can be applied at a large scale to improve the biochemical, physiological, and nutraceutical values of medicinal and crop sprouts.

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“…Dehydration often reduces the overall plant growth (Caser et al, 2012(Caser et al, , 2016(Caser et al, , 2017(Caser et al, and 2018Soni and Abdin, 2017), attributable to the considerable reduction of photosynthesis, cell turgidity, cell growth, and to the increasing evapotranspiration (Rahimi et al, 2017). This was seen in many MAPs, such as Mentha pulegium (Hassanpour et al, 2014), M. piperita (Rahimi et al, 2017), M. spicata (Delfine et al, 2005) and Rosmarinus officinalis (Delfine et al, 2005). Within the genus Salvia, different morphological responses to drought are reported in literature.…”

Section: Plant Growthmentioning

confidence: 99%

Drought stress adaptation modulates plant secondary metabolite production in Salvia dolomitica Codd

Caser

Chitarra

D’Angiolillo

et al. 2019

Industrial Crops and Products

100

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Sages are important medicinal and aromatic plants. While Salvia officinalis and S. miltiorrhiza have been widely studied, poor information are available on S. dolomitica which is recently addressing attention for its antiplasmodial and anti-inflammatory properties. The current study investigated the performances and the metabolic profile of this minor species in response to drought stress. Plants were exposed to three treatments: control, moderate drought stress, or severe drought stress.Changes in growth and ecophysiological traits, in active and volatile compounds, and essential oils production were determined. As the terpenoids are the most representative class of secondary metabolites, the gene expression of key enzymes of terpenoid biosynthesis has been also investigated. Moderate drought induced a drop of leaf water potential, growth, and stomatal conductance, while an increase of deyhdrin expression level. However, serious stress symptoms such as decrease of net photosynthesis and transpiration rate and increase of endogenous abscisic acid were observed only in severe drought stressed plants. Both drought stress conditions lead to modulate the expression of some genes involved in BVOCs and EOs biosynthesis and the metabolic profile. In particular, drought induced an increase of sesquiterpenes, a class of terpenoids with high importance in food, cosmetic and pharmaceutical industry. Thus, we can speculate that moderate drought stress, in addition to allowing water savings during cultivation, can lead to an improvement in the production of secondary metabolites in S. dolomitica.

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Effect of penconazole and drought stress on the essential oil composition and gene expression of Mentha pulegium L. (Lamiaceae) at flowering stage

Cited by 33 publications

References 23 publications

Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L.

Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L.

Laser Light Treatment of Seeds for Improving the Biomass Photosynthesis, Chemical Composition and Biological Activities of Lemongrass Sprouts

Drought stress adaptation modulates plant secondary metabolite production in Salvia dolomitica Codd

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