Effect of Pelargonidin isolated from Ficus benghalensis L. on phenotypic changes in zebrafish (Danio rerio) embryos

Abstract

In the present study, the extraction and isolation of Pelargonidin, an anthocyanin compound from stem bark of are described The study also involves evaluation of the effect of Pelargonidin on phenotypic variations in zebra fish embryos. Extraction and isolation of Pelargonidin were carried out by employing liquid-liquid extraction technique, phytochemical tests, column chromatography, UV and FT-IR. In the zebra fish embryo model, Paclitaxel was employed as a negative control. A series of phenotypic changes in different stages of embryonic development were studied with treatment concentrations of Pelargonidin between 3.0 and 20 ppm at 0-72-hour post-fertilization (hpf). The results of our studies indicate that, after exposure of zebra fish embryos to 3.3-20 ppm concentration of Pelargonidin for 72 h, a significant reduction in aortic development occurs. At the dose level of 0.5 ppm Paclitaxel and Pelargonidin in the dose range between 3.3 and 20 ppm, the zebra fish embryos were found to have bent tail, malformed eyes and developmental delays in vasculature. Based on the results obtained, we infer that Pelargonidin can exhibit phenotypic anti-angiogenic variations in embryonic stage of fish embryos and it can be applied in future for exploration of its anti-angiogenic potential. Furthermore, Pelargonidin could serve as a candidate drug for inhibition of angiogenesis and can be applied for the treatment of neovascular diseases and tumor.

Behavioural and pharmacological profiles of zebrafish administrated pyrrolidinyl benzodioxanes and prolinol aryl ethers with high affinity for heteromeric nicotinic acetylcholine receptors

“…Each fish was used only for one test. Ten fish per group were used for each drug concentration based on our (Braida et al 2014a(Braida et al , 2014bPonzoni et al, 2014Ponzoni et al, , 2016aPonzoni et al, , 2016b and on other studies (Karnik et al 2012;Kundap et al 2017) and each fish only received a single dose of one compound.…”

Zebrafish as a Model for Epilepsy-Induced Cognitive Dysfunction: A Pharmacological, Biochemical and Behavioral Approach

“…The cost and time required to carry out the study in zebrafish model is less as compared to rodents ( Mussulini et al, 2013 ). For studying various brain disorders, zebrafish ( Danio rerio ) is quickly mounting as a promising model creature ( Kundap et al, 2016 ). It is important to minimize animal distress by using the least sensitive organism possible to answer the question at hand.…”

<i>Ficus benghalensis</i> : A plant with potential pharmacological properties f rom tradition to pharmacy

“…The anti-angiogenetic potential of pelargonidin, an anthocyanin compound obtained from Ficus bengalensis was evaluated in an in vivo Danio rerio animal model. Pelargonidin significantly reduced the aortic development which clearly declares that pelargonidin have potential antiangiogenetic properties [15]. The anti-cancer mechanism of action of pelargonidin proceeds via decreased levels of H2O2 and TNFα-induced VEGF, VEGF inhibition or down-regulation of VEGF receptor expression.…”

“…Anti-cancer activity, anti-diabetic activity, ulcer pain inhibitors, free radical scavenging activity [14][15][16][17] α-sitosterol-α-6-Hepta triacontene-10-one penta triacontan-5-one mesoinositol phenyl tetra decanyl oleiate Larvicidal Activity (methanol extracts), free radical scavenging activity [8,13,17] Molecular targets involved in anti-cancer activity are transcription factors (API, NF-κB, NRF2), growth factors (EGF, FGF, PDGF), cell cycle proteins (cyclin D, CDK1, CDK2, p53, p27, p21), pro-apoptotic proteins (caspases and Bax), antiapoptotic proteins (bcl-2, TRAFI and survivin), and protein kinases (PKA, PKC, MAPK and TyK2) [18] (Table 2).…”