Effect of Peganum harmala L. extract supplemented ISP2 medium on growth and production of secondary metabolites of Streptomyces ayarius S115

Amel, Nait Marzoug; Adel, Ayari; Khaldi, Fadila; Ines, Guehria; Abdelhak, Gheid

doi:10.1016/j.ejbt.2022.12.006

Cited by 1 publication

(2 citation statements)

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“…The antibacterial activity of CF51DZ was tested by diffusion on agar wells. 8 In brief, wells of 6 mm in diameter were formed by a sterile well driller on Muller Hinton agar medium previously inoculated with pathogenic bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213) obtained from the Laboratory of Organic Chemistry and Interdisciplinary (Research's Team: valorization of active biomolecules), University of Souk-Ahras, Algeria. After centrifugation at 8000 rpm/20 min, 80 μl of supernatant) of liquid culture of strain CF51DZ was deposited in well.…”

Section: Antibacterial Activity Of Strain Cf51dzmentioning

confidence: 99%

“…6,7 Discovery of new secondary metabolites as bioactive molecules in unexplored habitats is pointed out as strategic actions to fight against the growing number of microorganisms that are resistant to a wide range of antibiotics obtained from actinomycetes. 8 Many actinobacteria, including Streptomycetes have been isolated as appropriate defensive partners associated withvarious types of insects, 9 and in this regard, Streptomyces and Pseudonocardia were reported to protect the leaf-cuttingants against pathogenic fungus. 10 Additionally, these insect-associated actinomycetes are possibly found in various female beewolf wasps, providing a specialized antennal reservoir for cultivating Streptomyces philanthi, and cover the cocoon wall to protect larva against pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Antibacterial Activity of Streptomyces mediolani CF51DZ Isolated from the Beetle (Protaetia morio), Collected from Souk Ahras, North-East of Algeria

Raffa,

Ayari,

Guehria

2024

J. Pure Appl. Microbiol.

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In this study, an Actinomycetota Streptomyces mediolani strain CF51DZ was isolated from a beetle Protaetia morio collected in a cork oak forest in northeast Algeria, to extract, purify and characterize the antibacterial compound. The strain was isolated in specific ISP culture media, identified via morphological, biochemical, physiological and molecular characteristics and then studied its antibacterial activities against pathogenic bacteria using the well diffusion technique. The extraction, purification and characterization of the bioactive compound was carried out using chromatographic and spectroscopic methods. Morphological, physiological, and biochemical properties, as well as the 16S ribosomal DNA sequence of the strain, were found close to those of Streptomyces mediolani. The isolated strain produced an active compound having potent antibacterial properties against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. This compound was isolated and purified with thin layer chromatography and high-performance liquid chromatography, and chemically identified by mass spectroscopy and NMR spectroscopy. In addition, the chemical structure elucidation of the antibacterial metabolite by APCI-MS analysis revealed a peak at m/z 375 [M+H]+, a molecular formula of C19H18O8. This compound was identified as methyl rosarinate: 3-(3,4-dihydroxyphenyl)-1-methoxy-1-oxopropan-2-yl (E)-3-(3,4-dihydroxyphenyl)acrylate based on the IUPAC nomenclature. Moreover, the antibacterial agent was purified from Streptomyces mediolani strain CF51DZ that was for the first time, in Algeria, isolated from the beetle Protaetia morio. This supports further characterization of this promising chemical to ensure the best use for antibacterial applications.

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Section: Antibacterial Activity Of Strain Cf51dzmentioning

confidence: 99%