Effect of PD98059 on the proliferation and apoptosis of pancreatic cancer Panc-1 cells and tumor growth in Panc-1-xenografted nude mice

Benzophenone Compounds, from a Marine-Derived Strain of the Fungus Pestalotiopsis neglecta, Inhibit Proliferation of Pancreatic Cancer Cells by Targeting the MEK/ERK Pathway

“…To examine whether these benzophenone compounds activate apoptotic signaling in PANC-1 pancreatic cancer cells, we detected the expression of apoptosis-related proteins (caspase-3 and poly­(ADP-ribose)­polymerase (PARP)) after treating the cells with various doses (0, 5, 10, 15 μM) of the two compounds for 24 h. As shown in Figure C, compounds 2 and 4 induced the cleavage of caspase-3 and PARP in a dose-dependent manner, which suggested that they promote apoptosis in PANC-1 pancreatic cancer cells. PD98059, a potent MEK1/2 inhibitor used as a positive control in our experiment, was reported to activate apoptosis factors at 50 μM in pancreatic cancer cells …”

“…PD98059, a potent MEK1/2 inhibitor used as a positive control in our experiment, was reported to activate apoptosis factors at 50 μM in pancreatic cancer cells. 30 To investigate the working mechanisms of compounds 2 and 4, molecular docking analysis was performed. The binding energy and poses of compounds 2 and 4 in the crystal structure of MEK1 (PDB ID: 1S9J) with the existence of ATP molecule were examined by Autodock4 software.…”