Effect of passive sensitization on the mechanical activity of human isolated bronchial smooth muscle induced by substance P, neurokinin A and VIP

Ben‐Jebria, Abdellaziz; Marthan, Roger; Rossetti, Max; Savineau, Jean‐Pierre

doi:10.1111/j.1476-5381.1993.tb13542.x

Cited by 49 publications

(42 citation statements)

References 33 publications

(44 reference statements)

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“…The response of the strips to contractile or relax ant agents may be more sensitive when preparations of a smaller size are used, since the technical difficulties due to the diffusion barrier could be reduced somewhat. A simi lar explanation was offered by Ben-Jebria et al [26] about the differences in the data between their study and other previous studies [27,28] regarding the effects of tachvkinins on human airway smooth muscle. They suggested that one possible reason for the interexaminer differences may be a variability in the responses to neuropeptides accord ing to the sizes of the preparations.…”

Section: Discussionmentioning

confidence: 53%

Cultured Epithelial Cells Release Cyclooxygenase-Dependent and Cyclooxygenase-lndependent Factors That Inhibit Cholinergic Contraction of Canine Airway Smooth Muscles

et al. 1996

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We observed the effects of supernatants from cultured epithelial cellson the contraction of tracheal smooth muscle evoked by acetylcholine (ACh) or by electrical field stimulation (EFS). Cultured canine tracheal epithelial cells were incubated in Krebs solution with or without indomethacin (10^-5 M) for 30 and 120 min. The amplitude of the tracheal smooth muscle contractions evoked by EFS or exogenously applied ACh were measured before and after the application of each supernatant in the combined presence of both indomethacin (10^-5M) and propranolol (10^-6 M). The control supernatant incubated without indomethacin markedly suppressed the amplitude of the contraction evoked by EFS, but not by ACh. The supernatant incubated with indomethacin for 30 min did not show any effects on the contractile responses evoked by EFS or ACh. However, the supernatants from the cultured epithelial cells incubated for a longer period (120 min) in the presence of indomethacin significantly suppressed the contraction evoked by EFS, but not by ACh. The prostaglandin E₂ (PGE₂) concentration was markedly higher in the supernatants incubated without indomethacin (1.39 ± 0.51 ng/ ml, 30 min incubation) than in those with indomethacin (0.02 ± 0.01 ng/ml, 30 min incubation, and 0.06 ± 0.01 ng/ml, 120 min incubation). To determine whether PGE₂ is responsible for the inhibitory effect of the supernatants, we evaluated the effects of PGE₂ on the resting tone, and EFS- or ACh-evoked contraction. 10^-12 to 10^-6M of PGE₂ showed no significant effect on the resting tone. 10^-9 M of PGE₂, corresponding to the concentration of the supernatants incubated without indomethacin, and 10^-11M of PGE₂, to that of the supernatants incubated with indomethacin, showed a similar extent of inhibitory effects to the corresponding supernatants on the EFS-evoked contraction, and no effect on the ACh-evoked contraction. These results suggest that cultured airway epithelial cells release at least two factors spontaneously even without stimulation. One of these factors may be prostanoid (PGE₂), which acts prejunctionally to inhibit the contractile response. The other factor is distinct from prostanoid and inhibits smooth muscle contraction, presumably by suppressing ACh release from vagus nerve termini.

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Section: Discussionmentioning

confidence: 53%

Cultured Epithelial Cells Release Cyclooxygenase-Dependent and Cyclooxygenase-lndependent Factors That Inhibit Cholinergic Contraction of Canine Airway Smooth Muscles

et al. 1996

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“…The chamber was filled with physiologic solution (composition in mM: 130 NaCl, 5.6 KCl, 1 MgCl 2 , 2.5 CaCl 2 , 11 D -glucose buffered by Tris-HCl to pH 7.4 at 30 Њ C). Tissues were set at optimal length by equilibration against a passive load of 40 mg as determined previously for this type of preparation (18). At the beginning of each experiment, K ϩ -rich (110 mM) solution, was repeatedly applied to obtain at least two contractions similar in both amplitude and kinetics.…”

Section: Isometric Contraction Measurementmentioning

confidence: 99%

Human Isolated Bronchial Smooth Muscle Contains Functional Ryanodine/Caffeine–sensitive Ca-Release Channels

Hyvelin

Martin

Roux

et al. 2000

Am J Respir Crit Care Med

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Human bronchial smooth muscle (HBSM) contraction is implicated in a variety of respiratory diseases, including asthma. Yet, the presence of an operative calcium-induced calcium release (CICR) mechanism, identified in various smooth muscles, has not been established in HBSM. We therefore studied Ca-releasing mechanisms in HBSM obtained at thoracotomy with special attention to ryanodine-sensitive receptor channels (RyRs). In freshly isolated bronchial myocytes, ryanodine (0.5 to 50 microM) and caffeine (1 to 25 mM) induced transient increases in the cytoplasmic calcium concentration ([Ca(2+)](i)). Higher ryanodine concentrations (> 100 microM) inhibited the caffeine-induced [Ca(2+)](i) response, which was also blocked in the presence of tetracaine (300 microM) or ruthenium red (200 microM), two potent CICR inhibitors. In HBSM strips, caffeine induced a transient contraction which, likewise, was inhibited by ryanodine and tetracaine. However, ryanodine (200 microM) modified neither the [Ca(2+)](i) response nor the contraction induced by K(+)-rich (110 mM) solution. Reverse transcriptase/polymerase chain reaction (RT-PCR) and RNase protection assay performed in HBSM have revealed the existence of mRNAs encoding only the type 3 RyR. We also characterized acetylcholine-induced [Ca(2+)](i) and contractile responses. None of these responses was altered by ryanodine or by tetracaine. These results demonstrate, for the first time, the existence of functional RyRs in HBSM cells which, owing to the type of isoform or the amount of protein expressed, are not involved, under physiologic conditions, in depolarization- or agonist-induced contraction.

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“…25 A recent report has examined the eVect of passive sensitisation on the in vitro contractile eVect of SP and NKA on human bronchi. 26 Human bronchi incubated overnight with serum from asthmatic patients sensitised to HDM showed an enhanced sensitivity and an enhanced maximal contractile response to both tachykinins, an eVect which was independent of changes in the activity of neutral endopeptidase. Thus, sensitised airway smooth muscle is more sensitive to tachykinins.…”

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confidence: 99%

Neurokinin A is the predominant tachykinin in human bronchoalveolar lavage fluid in normal and asthmatic subjects

Heaney

Cross

McGarvey

et al. 1998

Thorax

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Background-Multiple sensory neuropeptides are present in human airways and may contribute to diseases such as asthma. This study quantified and characterised substance P (SP), neurokinin A (NKA), and calcitonin gene related peptide (CGRP) immunoreactivity in bronchoalveolar lavage fluid in asthmatic and normal subjects. Methods-Using specific radioimmunoassay (RIA), SP, NKA and CGRP were measured in bronchoalveolar lavage fluid from asthmatic subjects (n = 5), normal subjects (n = 5), atopic non-asthmatic subjects (n = 6), and asthmatic subjects four hours after allergen challenge (n = 12). Peptide immunoreactivity was characterised using high performance liquid chromatography (HPLC) and RIA. Results-No SP or CGRP immunoreactivity was detected in any of the fractions from samples after extraction, HPLC, and RIA. Non-specific binding resulted in spurious SP immunoreactivity being detected in bronchoalveolar lavage fluid when no extraction process was employed. NKA was detected in significant amounts in asthmatic (median 550, range 425-625 pg/ml) and normal subjects (median 725, range 350-1425 pg/ml). The level of NKA was significantly higher in the asthmatic subjects after allergen challenge (median 750, range 350-1250 pg/ml) than in unchallenged asthmatic subjects (median 600, range 425-600 pg/ml, p<0.01). Conclusions-Extraction and characterisation of peptides from bronchoalveolar lavage fluid must be performed to ensure that the measured immunoreactivity represents target peptide. NKA is present in bronchoalveolar lavage fluid in high concentrations and is the predominant tachykinin. The concentrations of NKA are similar in normal subjects and subjects with mild asthma. (Thorax 1998;53:357-362)

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Effect of passive sensitization on the mechanical activity of human isolated bronchial smooth muscle induced by substance P, neurokinin A and VIP

Cited by 49 publications

References 33 publications

Cultured Epithelial Cells Release Cyclooxygenase-Dependent and Cyclooxygenase-lndependent Factors That Inhibit Cholinergic Contraction of Canine Airway Smooth Muscles

Cultured Epithelial Cells Release Cyclooxygenase-Dependent and Cyclooxygenase-lndependent Factors That Inhibit Cholinergic Contraction of Canine Airway Smooth Muscles

Human Isolated Bronchial Smooth Muscle Contains Functional Ryanodine/Caffeine–sensitive Ca-Release Channels

Neurokinin A is the predominant tachykinin in human bronchoalveolar lavage fluid in normal and asthmatic subjects

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