Effect of Particulate Materials on Population Growth of the Free-Living Nematode Caenorhabditis briggsae

Cheng, Andrew; Lu, Nancy C.; Briggs, George M.; Stokstad, E. L. R.

doi:10.3181/00379727-160-40420

Cited by 10 publications

(6 citation statements)

References 5 publications

(6 reference statements)

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“…The C. elegans dependence on particulate matter present on milk led us to question whether this was due to a nutritional or physical factor. Casein micelles were a useful target for this analysis as they are present in milk, and could provide a protein source and/or a physical transport vessel for nutrients ( Cheng et al 1979 ; Enright et al 1999 ; Brans et al 2004 ). Casein micelles share a very similar range of sizes and morphology to bacteria, in addition to parallel behaviors such as aggregation ( Enright et al 1999 ).…”

Section: Resultsmentioning

confidence: 99%

Growth ofCaenorhabditis elegansin Defined Media Is Dependent on Presence of Particulate Matter

Flavel

Mechler

Shahmiri

et al. 2018

G3 Genes|Genomes|Genetics

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Caenorhabditis elegans are typically cultured in a monoxenic medium consisting of live bacteria. However, this introduces a secondary organism to experiments, and restricts the manipulation of the nutritional environment. Due to the intricate link between genes and environment, greater control and understanding of nutritional factors are required to push the C. elegans field into new areas. For decades, attempts to develop a chemically defined, axenic medium as an alternative for culturing C. elegans have been made. However, the mechanism by which the filter feeder C. elegans obtains nutrients from these liquid media is not known. Using a fluorescence-activated cell sorting based approach, we demonstrate growth in all past axenic C. elegans media to be dependent on the presence of previously unknown particles. This particle requirement of C. elegans led to development of liposome-based, nanoparticle culturing that allows full control of nutrients delivered to C. elegans.

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Section: Resultsmentioning

confidence: 99%

Growth ofCaenorhabditis elegansin Defined Media Is Dependent on Presence of Particulate Matter

Flavel

Mechler

Shahmiri

et al. 2018

G3 Genes|Genomes|Genetics

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“…Particulate material, in the form of shed cuticles and dead animals, accumulates in conditioned liquid CeMM. Particulate material has been reported to increase the growth rate of the related species C. briggsae by providing physical stimulation [12]. To test if particulate material accounted for the increased growth rate we grew animals in conditioned, heat killed liquid CeMM subject to filtration through a 0.2 micron filter (Millipore).…”

Section: Resultsmentioning

confidence: 99%

Chemically defined medium and Caenorhabditis elegans

2003

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“…Furthermore, earlier studies have suggested that C. elegans digestion involves endocytic processes that depend on particles of the size of microbial or subcellular particles generated by the action of the pharyngeal grinder (20,21). The uptake of nutrients in the gut is stimulated by the presence of particles in the culture medium (21,40). These particles are missing from axenic culture, possibly resulting in decreased nutrient uptake.…”

Section: Discussionmentioning

confidence: 99%

Dietary Restriction of Caenorhabditis elegans by Axenic Culture Reflects Nutritional Requirement for Constituents Provided by Metabolically Active Microbes

Lenaerts

Walker²,

Hoorebeke³

et al. 2008

The Journals of Gerontology Series A: Biological Sciences and M

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In Caenorhabditis elegans, several manipulations that affect nutrition slow development, reduce fecundity, and increase life span. These are viewed as dietary restriction (DR) and include culture in semidefined, nutrient-rich liquid medium that is axenic (i.e., there is no microbial food source). Here we describe convenient ways to exert DR by culture on agar plates containing axenic medium. We used these to explore whether effects of axenic culture really reflect DR. Our results imply that major nutrient components of axenic medium, and overall caloric content, are not limiting for life span. However, adding growth-arrested Escherichia coli as an additional food source rescued the effects of axenic culture. We then sought to identify the component of E. coli that is critical for normal C. elegans nutrition using add-back experiments. Our results suggest that C. elegans has a nutritional requirement for live, metabolically active microbes or, possibly, an unidentified, heat-labile, nonsoluble component present in live microbes.

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Effect of Particulate Materials on Population Growth of the Free-Living Nematode Caenorhabditis briggsae

Cited by 10 publications

References 5 publications

Growth ofCaenorhabditis elegansin Defined Media Is Dependent on Presence of Particulate Matter

Growth ofCaenorhabditis elegansin Defined Media Is Dependent on Presence of Particulate Matter

Chemically defined medium and Caenorhabditis elegans

Dietary Restriction of Caenorhabditis elegans by Axenic Culture Reflects Nutritional Requirement for Constituents Provided by Metabolically Active Microbes

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