Effect of Partially Purified Enterocins from &lt;i&gt;Enterococcus faecalis&lt;/i&gt; Strains on the Growth of Some Phytopathogenic Fungi

David, Oluwole Moses; Onifade, O.E.

doi:10.4038/rjs.v9i2.44

Cited by 5 publications

(4 citation statements)

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“…against P. variotii , R. cerealis , A. tenuissima , and C. cladosporioides . However, the inhibitory effects of enterococci on other tested fungi species, such as A. niger , A. alternata , and P. expansum , were already documented in existing literature (Fhoula et al, 2013 ; Ben Braïek et al, 2018; David and Onifade, 2018 ; Zabouri et al, 2021 ), corroborating the findings of our experiments, which showcased similar antagonistic properties.…”

Section: Discussionsupporting

confidence: 92%

“…Enterococci have been observed to produce phytohormones (Lee et al, 2015) and bacteriocins with different activity spectra (Okkers et al, 1999;H-Kittikun et al, 2015;Almeida-Santos et al, 2021). They can also synthesize biosurfactants, volatile compounds, and other substances that protect plants against phytopathogens, making them potential biocontrol agents (Kumar et al, 2016;David and Onifade, 2018;Maany et al, 2019;Chaurasia et al, 2021;Diaz et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Tytarenko,

Tesliuk,

Merlich

et al. 2023

bta

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Clonal micropropagation is an effective method for plant reproduction, applicable in both scientific and industrial domains. However, a significant number of microclones are lost during the ex vitro acclimatization process. To address this, the introduction of beneficial microorganisms into the rhizosphere of micropropagated plants could have a positive effect on the survival rates and external characteristics of acclimatized plantlets. The aim of this study was to determine the protective and growth-promoting potential of Enterococcus italicus ONU547 and its effect on micropropagated plants during acclimatization. The antagonistic activity of the bacteria was determined using the agar block method. Lepidium sativum L. seeds were inoculated with bacterial suspensions at concentrations of 10 6 , 10 7 , and 10 8 CFU/ml. Subsequently, the roots of the microclones were treated with suspensions of 10 6 and 10 7 CFU/ml, and biometric characteristics were measured. The results demonstrated antagonistic properties against various phytopathogenic fungi, including Aspergillus niger , Cladosporium cladosporioides , Alternaria alternata , Alternaria tenuissima , Rhizoctonia cerealis , Penicillium expansum , and Paecilomyces variotii . Inoculation of L. sativum L. seeds resulted in improved germination rates, increased root numbers, and enhanced root and shoot lengths. Similarly, the effects of the studied bacteria on Rubus fruticosus L. and Paulownia tomentosa Steud. during the acclimatization stage led to higher survival rates, increased shoot lengths, greater node numbers, and larger leaf areas. A concentration of 10 7 CFU/ml was identified as optimal for inoculating the microclones. The findings indicate that E. italicus ONU547 holds promise for the inoculation of micropropagated plants during the acclimatization process. Further research is recommended to establish the specific interaction mechanisms between these bacteria and plants.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Tytarenko,

Tesliuk,

Merlich

et al. 2023

bta

View full text Add to dashboard Cite

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“…The genus Enterococcus is a member of LAB and widespread in nature. They are part of the gastrointestinal flora and are detected in samples from all over the animal kingdom, insects, lower vertebrates, and humans (David et al 2012;David and Onifade 2018). They produce small, extracellular metabolites that have been reported against food-borne pathogens and, more recently, against plant pathogens (Belguesmia et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Production of Antifungal Gelatinase by Enterococcus durans S2C and Its Potential Role in the Biological Control

Hayat¹

2021

IJAB

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Probiotic bacteria are good sources for antimicrobial and are normal microbiota of the gastrointestinal tract of animals and humans. Enterococci are found everywhere in nature and have been utilized as probiotics in the food industry. In the present study, Enterococcus durans S2C strain was isolated from raw cow’s milk using the culture-dependent method and evaluated for antifungal properties. The strain S2C showed promising extracellular proteolytic activity and the extracellular peptide was an important source of antifungal activity. Besides, a low rate of antibiotic resistance and non-hemolytic activity was detected in E. durans S2C. Strong antifungal activity of E. durans against two plant pathogenic fungi namely Fusarium oxysporum and Rhizoctonia solani was also recorded. Antifungal activity evaluation showed that R. solani colony was the most inhibited fungus followed by F. oxysporum. Gelatinase enzyme having antifungal activity was also purified from E. durans S2C isolate. This study concludes that the environmental-derived E. durans S2C strain can be selected for technological application to control pathogenic fungi and to protect the economic value of the crops. © 2021 Friends Science Publishers

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“…Recent studies have focused on the application of bacteriocins to control phytopathogens (Fernandez, Godino, Príncipe, Morales, & Fischer, 2017;Lopes et al, 2017;Príncipe, Fernandez, Torasso, Godino, & Fischer, 2018), demonstrating an alternative strategy to the use of chemical inputs in agriculture. These potential alternatives range from attacking fungi (Benitez, Velho, Lisboa, da Costa Medina, & Brandelli, 2010;David & Onifade, 2018), to bacteriocins with a lethal effect against the bacteria responsible for great economic agricultural damage (Jeong, Jo, Hong, & Park, 2016;Príncipe et al, 2018). Studies have also suggested the use of these antimicrobial peptides as plant growth/development biostimulants and protection against abiotic stresses (Murthy et al, 2016;Nazari & Smith, 2020;Prudent, Salon, Souleimanov, Emery, & Smith, 2015).…”

mentioning

confidence: 99%

Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans

Ramos

Meneses

Vidal

et al. 2021

Annals of Applied Biology

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Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopathogenic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 αhelices and 11 β-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 μg μl À1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials.Scanning electron microscopy images showed that Gluconacin-treated X. albilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis.Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.

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Effect of Partially Purified Enterocins from <i>Enterococcus faecalis</i> Strains on the Growth of Some Phytopathogenic Fungi

Cited by 5 publications

References 0 publications

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Production of Antifungal Gelatinase by Enterococcus durans S2C and Its Potential Role in the Biological Control

Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans

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