Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics

Miyatake, Naohisa; Kishimoto, Koshi N.; Anada, Takahisa; Imaizumi, Hideki; Itoi, Eiji; Сузукі, Осаму

doi:10.1016/j.biomaterials.2008.10.058

Cited by 121 publications

(138 citation statements)

References 45 publications

(77 reference statements)

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“…The effect of calcium and Pi extracellular ion concentration has also been reported to affect osteoblast differentiation 35,36) . Studies using OCP also suggested that the osteoconductivity is induced by multiple factors: (i) the elevated extracellular calcium and Pi in the medium accompanied by OCP-HA conversion 16) ; (ii) the morphology of the crystals 37) , and (iii) the subtle change in the Ca/P molar ratio (the non-stoichiometry of the chemical composition) 38) . OCP is more soluble than β-TCP and HA 15) .…”

Section: Discussionmentioning

confidence: 99%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

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Section: Discussionmentioning

confidence: 99%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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“…It has been proposed that the structure of a non-stoichiometric OCP contains an excess of hydrogen, resulting in a non-stoichiometric chemical formula Ca 16 H 4?x (PO 4 ) 12 (-OH) x Á(10 -x)H 2 O, which resembles the structure of HA even more closely than previously anticipated (Mathew et al 1988). Furthermore, a partially hydrolyzed form of OCP with Ca/P molar ratio of 1.37 might be prepared (Suzuki 2010a;Miyatake et al 2009). At solution pH = 7.2 and temperature 60°C, the full hydrolysis of OCP into CDHA occurs within *6 h ( Arellano-Jiménez et al 2009).…”

Section: Dcpa (Or Dcp)mentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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“…7,16 A recent study confirmed that a subtle change in the stoichiometry of OCP, caused by partial hydrolysis, markedly affects its osteoconductive and biodegradable characteristics in rat tibia bone marrow. 28 The results suggest that OCP promotes osteoblastic cell differentiation induced by the process of OCP-HA conversion if implanted in bony sites where osteoblasts are present, resulting in the enhancement of bone formation.…”

Section: Effct Of Ocp In Osteoblast Differentiationmentioning

confidence: 81%

Transitory Nature of Octacalcium Phosphate in Physiological Milieu and Its Osteoconductive Property

Сузукі

2009

Phosphorus Research Bulletin

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Octacalcium phosphate (OCP) is a metastable salt in physiological pH which tends to convert to hydroxyapatite (HA). Conversion from OCP to HA is also observed if OCP is implanted in various bony sites. From a quantitative viewpoint, the implantation of OCP granules enhances bone regeneration more than that of non-sintered HA or sintered HA granules in rabbit bone marrow spaces or the subperiosteal area of mouse calvaria; in addition, OCP is biodegraded by osteoclast-like cells. OCP promotes differentiation of bone marrow stromal cells to osteoblastic cells. These osteoconductive characteristics may be acquired by the dissolution process associated with the progressive OCP-HA conversion. The biodegradability and osteoconductive characteristics are summarized in relation to the solubility of OCP.

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Effect of partial hydrolysis of octacalcium phosphate on its osteoconductive characteristics

Cited by 121 publications

References 45 publications

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Transitory Nature of Octacalcium Phosphate in Physiological Milieu and Its Osteoconductive Property

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