Effect of parthenocarpy on ovule development in cucumber (Cucumis sativus L.) after pollination with normal and irradiated pollen

Deunff, Erwan Le; Sauton, A.

doi:10.1007/bf00232741

Cited by 14 publications

(4 citation statements)

References 38 publications

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“…A number of studies have shown that after pollination with irradiated pollen, seeds with only endosperm are produced in apple (James et al 1985;Nicoll et al 1987;Zhang and Lespinasse 1991), cacao (Falque et al 1992), cucumber (Le Deunff and Sauton 1994) and kiwifruit (Musial and Przywara 1998). In vitro culture of unfertilized ovules induced autonomous endosperm in cotton (Jensen et al 1977), barley (Huang et al 1982), rice (Zhou and Yang 1981), Melandrium album, Helleborus niger and Lupinus luteus (Mól 1992;Mól et al 1995), and Brassica napus (Chmielowiec et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Autonomous endosperm induction by in vitro culture of unfertilized ovules of Viola odorata L.

Wijowska¹,

Kuta²,

Przywara³

1999

Sexual Plant Reproduction

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Autonomous endosperm was found in unfertilized ovules of V. odorata L. cultured on MS medium supplemented with 2,4-D as a sole growth regulator or on media with 2,4-D and BAP or kinetin. Frequency of endosperm induction was approximately 9% in ovules analyzed. The induction rate depended mainly on genotype of the donor plant, and to lesser degrees, on floral stage, flower series and medium type. Multinuclear endosperms consisting of 10-37 nuclei were found in ovules after as few as 4 days of culture. In some ovules at this stage, the egg cell and two polar nuclei were present. The process of endosperm degeneration began after 3 weeks of culture. In some ovules, degenerating autonomous endosperm was observed up to the 7th week. Parthenogenetic development of egg cells or apogamy did not accompany autonomous endosperm, supporting the hypothesis of independent pathways for embryo and endosperm development.

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Section: Introductionmentioning

confidence: 99%

Autonomous endosperm induction by in vitro culture of unfertilized ovules of Viola odorata L.

Wijowska¹,

Kuta²,

Przywara³

1999

Sexual Plant Reproduction

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“…At the Department of Plant Genetics, Breeding and Biotechnology cucumber seeds are inspected over a light source and the transparent (empty) seeds are discarded. Another efficient method of embryo detection is the application of X-ray radiography of seeds (Savin et al 1988, Le Deunff and Sauton 1994, Claveria et al 2005, Dolcet-Sanjuan et al 2006, which also enables the detection of seeds containing developing embryos. Lofti et al (2003) and Lofti and Salehi (2008) show that about 10 days of culture of seeds in liquid E20A medium, prior to embryo isolation, increases the number of haploid plants, likely due to the increased visibility of the embryos.…”

Section: Embryo Detection Excision and Culturementioning

confidence: 99%

Review of research on haploid production in cucumber and other cucurbits

Gałazka

Niemirowicz-Szczytt

2013

Folia Horticulturae

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This review provides a summary of haploid induction methods and factors affecting the efficacy of specific methodologies as applied to cucumber (Cucumis sativus L.), melon (Cucumis melo L.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai), winter squash (Cucurbita maxima Duch. ex Lam.), summer squash (Cucurbita pepo L.) and other cucurbits. This report is focused on studies that were carried out during the last 20 years. The main objective of the research on the production of haploid cucurbit plants is to accelerate breeding programs through the use of homozygous double haploid lines (DHL) and to facilitate the selection of desired (e.g. disease-resistant) genotypes for breeding. Unfortunately, currently used protocols result in a low number of double haploids (DH). The most common and best-known method of obtaining haploid cucurbit plants is via pollination with irradiated pollen, which induces parthenogenetic development of haploid embryos in planta. The embryos are extracted from immature seeds and cultured in vitro to facilitate the maturation and development of plants. The studies described below were primarily aimed at the determination of an appropriate dose of radiation and the evaluation of the impact of the genotype and the time of year on the number of haploid embryos and plants obtained. A less popular method of haploid production - ovule and ovary culture - is based on in vitro gynogenesis. The studies related to this method concentrated on optimising the composition of the medium and pre-treatment conditions (primarily temperature) to which the flower buds were subjected. Recently, increasing attention has been paid to anther and microspore culture. As in the case of in vitro ovule and ovary culture, the medium composition and flower bud pre-treatment conditions were optimised. The most recent studies suggest that anther culture is comparable in effectiveness to the irradiated pollen technique.

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“…However, in most parthenocarpic species or genotypes, there is evidence that parthenocarpy does not interfere with other processes of generative development. Ovule morphogenesis and female gamete production have been described as normal in genetically parthenocarpic watermelons (Buttrose and Sedgley 1979), tomatoes (Lin et al 1983) and cucumbers (Le Deunff and Sauton 1994). In contrast, the parthenocarpic fruit (pat) tomato mutation is responsible for a complex phenotype that involves stamen (reduced length and carpelloidy), ovule (defective integument growth and nonviability) and ovary (autonomous growth, i.e., parthenocarpy) development (Mazzucato et al 1998).…”

Section: Introductionmentioning

confidence: 97%

A defective pollen-pistil interaction contributes to hamper seed set in the parthenocarpic fruit tomato mutant

Mazzucato

Olimpieri

Ciampolini

et al. 2003

Sexual Plant Reproduction

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In the parthenocarpic fruit (pat) tomato mutant, parthenocarpy is associated with partial aberrations of stamens (shortness and carpelloidy) and ovules (defective integument growth) that contribute to impair seed set. However, these do not seem to be the only reasons for seed infertility because hand-pollination fails to restore seed set in ovaries where a fraction of the ovules are still morphologically normal. Therefore, it is conceivable that other unreported defects occur during the reproductive process in the mutant. In this research, we show that the mutation does not affect pollen or embryo sac development and viability, but generates sporophytic effects that reduce seed production and seed size. While pollen germination and stylar growth were normal in mutant pistils, fertilization does not take place because of abnormalities in the pollen tube-ovary interaction in this genotype. Inside the ovary of pat plants, pollen tubes appeared to be disorientated; they wandered about in the ovarian cavity and often lost their adherence to the placental surface. Interestingly, in pat ovaries fertilization was strongly impaired even in those ovules that appeared normal. It may be that apparently 'normal' ovules cannot guide pollen tubes to their micropyle in the altered pat ovary because adhesion molecules are not properly arrayed on a placenta that is already preparing for cell division or, alternatively, chemotropic signals in the pat ovary may be altered by the presence of aberrant ovules, which are not simply devoid of attractivity, but disrupt pollen tube guidance overall.

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Effect of parthenocarpy on ovule development in cucumber (Cucumis sativus L.) after pollination with normal and irradiated pollen

Cited by 14 publications

References 38 publications

Autonomous endosperm induction by in vitro culture of unfertilized ovules of Viola odorata L.

Autonomous endosperm induction by in vitro culture of unfertilized ovules of Viola odorata L.

Review of research on haploid production in cucumber and other cucurbits

A defective pollen-pistil interaction contributes to hamper seed set in the parthenocarpic fruit tomato mutant

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