Effect of parenteral immunization on the intestinal immune response to Salmonella typhi Ty21a

Forrest, B. D.; LaBrooy, Justin T.

doi:10.1016/0264-410x(92)90167-i

Cited by 2 publications

(3 citation statements)

References 15 publications

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“…Three doses (10 11 CFU) of S. typhi Ty21a elicited specific anti-LPS IgA in 70% of jejunal fluid samples from a large group of vaccinees (n ϭ 81). This rate of response reached 100% when smaller groups of vaccinees were considered (17,18). In contrast, only 13.6% (3 of 22) to 20% (1 of 5) of the vaccinees had some specific anti-LPS IgA in their saliva samples 2 weeks after immunization (14,15).…”

Section: Discussionmentioning

confidence: 97%

“…However, the three spaced doses used in our vaccination schedule did not greatly improve the overall immune response, as only one of the orally immunized volunteers (volunteer 5) responded after the third immunization. In contrast, three alternate daily oral doses of 10 11 CFU of S. typhi Ty21a or of S. typhi Ty21a expressing Vibrio cholerae LPS elicit seroconversion in 62 (17,28) and 100% of the vaccines, respectively (45).…”

Section: Discussionmentioning

confidence: 97%

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Oral and rectal immunization of adult female volunteers with a recombinant attenuated Salmonella typhi vaccine strain

et al. 1996

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An attenuated strain of Salmonella typhi ⌬cya ⌬(crp-cdt) ⌬asd expressing a gene encoding a hepatitis B virus core-pre-S protein was tested in female adult volunteers for its ability to elicit a systemic and a mucosal immune response. Specifically, our purpose was to evaluate the potential of such a vaccine strain to induce specific secretory immunoglobulin A (sIgA) at genital and rectal surfaces. Oral and rectal routes of immunization were compared: oral immunization induced seroconversion against the bacterial lipopolysaccharide (LPS) in six out of seven volunteers, while after rectal immunization only one out of six volunteers seroconverted against LPS. To our disappointment, the latter volunteer was also the only one who seroconverted against the carried antigen (pre-S1), demonstrating the poor ability of this live vaccine to induce an immune response against the carried antigen. Anti-LPS sIgA was found in both the vaginal and cervical secretions of a volunteer who presented a strong seroconversion after oral immunization (16-fold increase in anti-LPS IgG). Smaller amounts of anti-LPS sIgA were found in the rectal secretions of one orally and one rectally immunized volunteer and in the saliva of three orally and one rectally immunized woman. Our data show for the first time that it is possible to induce specific sIgA in the genital and rectal tracts of women by using an S. typhi vaccine strain.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Oral and rectal immunization of adult female volunteers with a recombinant attenuated Salmonella typhi vaccine strain

et al. 1996

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“…(22), E. coli heat-labile enterotoxin (23), and cholera toxin (30), showing that parenteral priming followed by a mucosal immunization enhanced mucosal immune responses and, in the cholera toxin study, protection. However, it should be noted that other studies have indicated that parenteral priming had no effect on subsequent immune responses (14) or was suppressive (15,29,31), although Pierce did observe that the suppressive effect of parenteral immunization was largely reversed by a subsequent enteric immunization (29). Since it has been suggested that priming for a mucosal response is affected by dosage number and interval as well as immunization route(s) (29), further studies to determine optimum immunization regimens should be made.…”

Section: Discussionmentioning

confidence: 99%

Local immune response and protection in the guinea pig keratoconjunctivitis model following immunization with Shigella vaccines

et al. 1994

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This study used the guinea pig keratoconjunctivitis model to examine the importance of route of administration (mucosal versus parenteral), frequency and timing of immunization (primary versus boosting immunization), and form of antigen given (live attenuated vaccine strain versus 0-antigen-protein conjugate) on the production of protective immunity against Shigella infection. Since local immune response to the lipopolysaccharide (LPS) 0-antigen of Shigella spp. is thought to be important for protection against disease, 0-antigenspecific antibody-secreting cells (ASC) in the spleen and regional lymph nodes of immunized animals were measured by using an ELISPOT assay. Results indicated that protective efficacy was associated with a strong 0-antigen-specific ASC response, particularly in the superficial ventral cervical lymph nodes draining the conjunctivae. In naive animals, a strong ASC response in the cervical lymph nodes and protection against challenge were detected only in animals that received a mucosal immunization. Protection in these animals was increased by a boosting mucosal immunization. While parenteral immunization alone with an 0-antigenprotein conjugate vaccine did not protect naive animals against challenge, a combined parenteral-mucosal regimen elicited enhanced protection without the addition of a boosting immunization. Although 0-antigenspecific serum immunoglobulin A titers were significantly higher in animals receiving a mucosal immunization, there was no apparent correlation between levels of serum antibody and protection against disease.

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Effect of parenteral immunization on the intestinal immune response to Salmonella typhi Ty21a

Cited by 2 publications

References 15 publications

Oral and rectal immunization of adult female volunteers with a recombinant attenuated Salmonella typhi vaccine strain

Oral and rectal immunization of adult female volunteers with a recombinant attenuated Salmonella typhi vaccine strain

Local immune response and protection in the guinea pig keratoconjunctivitis model following immunization with Shigella vaccines

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