Effect of parent genotype on somatic embryogenesis in Scots pine (Pinus sylvestris)

Niskanen, Anna-Maija; Lu, Jinrong; Seitz, Sakari; Keinonen, Kaija; Weissenberg, K. von; Pappinen, Ari

doi:10.1093/treephys/24.11.1259

Cited by 69 publications

(57 citation statements)

References 28 publications

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“…The initiation of somatic embryogenesis in conifers is under strong genetic control as has been mentioned earlier in the text (Niskanen et al 2004). Depending on the family, in Pinus sylvestris the initiation frequencies varied from 0 to 26.7% (Aronen et al 2009).…”

Section: Discussionmentioning

confidence: 86%

“…Many attempts have been done in order to improve the initiation of embryogenic tissues in conifers. The effort focused mainly on testing of nutrient medium com-position and plant growth regulators (Ewald et al 1995, Hazubska-Przybyl andBojarczuk 2008), carbon sources ( Salajova and Salaj 2005), vitamins (Pullman et al 2006), medium salt composition (Lelu et al 1994, Becwar et al 1990, Miguel et al 2004, Kim et al 2009, Krajňáková et al 2008, polyamines effect (Nakagawa et al 2011) as well as environmental (Vookova and Kormutak 2009) and genetic effects (Cheliak and Klim·aszewska 1991, Niskanen et al 2004, Yildirim et al 2006) have been studied.…”

Section: Introductionmentioning

confidence: 99%

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The effect of culture medium formulation on Pinus nigra somatic embryogenesis

Salaj¹,

Fráterová²,

Cárach³

et al. 2013

Dendrobiology

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Abstract:The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

The effect of culture medium formulation on Pinus nigra somatic embryogenesis

Salaj¹,

Fráterová²,

Cárach³

et al. 2013

Dendrobiology

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“…No entanto, a iniciação de culturas embriogênicas é conhecida por estar sob forte controle genético (PARK et al, 1998;LELU et al, 1999;KLIMASZEWSKA et al, 2001;PULLMAN et al, 2003;MIGUEL et al, 2004;NISKANEN et al, 2004;MACKAY et al, 2006;WALTER et al, 2006). Observa-se forte efeito materno na extrusão do megagametófito e, após a extrusão, efeito genético aditivo (PULLMAN et al, 2003;MACKAY et al, 2006;WALTER et al, 2006).…”

Section: Dias Pc Et Alunclassified

PARÂMETROS GENÉTICOS PARA A CAPACIDADE DE PROPAGAÇÃO DE Pinus taeda POR EMBRIOGÊNESE SOMÁTICA1

et al. 2015

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e Regiane Abjaud Estopa 5 RESUMO -Objetivou-se com este trabalho avaliar a capacidade de propagação de famílias de Pinus taeda por embriogênese somática utilizando estimativas de parâmetros genéticos. Para a embriogênese somática, foram selecionados cones imaturos de 65 famílias-elite de Pinus taeda. O germoplasma utilizado para a implantação dos testes de campo foi composto por 238 clones de 31 famílias. O estudo foi realizado por meio de análise genetíoco-estatística pelo procedimento de estimação de componentes de variância via máxima verossimilhança residual (Reml) e de predição de valores genéticos via melhor predição linear não viesada (Blup), usando-se o software Selegen-Reml/Blup. De acordo com os resultados, a variabilidade genética possibilita ganhos genéticos altos pela seleção entre famílias, para os caracteres presença de embriões somáticos e número de clones por famílias de Pinus taeda. Há baixa ou nenhuma correlação genética entre o número de clones propagados via embriogênese somática e as características altura, diâmetro, sobrevivência e volume avaliados aos 4 anos de idade em testes clonais. Conclui-se que há maior ganho genético para a capacidade de propagação por embriogênese somática com a seleção de famílias de Pinus taeda.Palavras-chave: Controle genético; Ganho genético; Propagação clonal. GENETIC PARAMETERS FOR PROPAGATION CAPACITY OF Pinus taeda BY SOMATIC EMBRYOGENESISABSTRACT -The objective of this work was to evaluate families propagation of Pinus taeda by somatic embryogenesis using estimates of genetic parameters. For somatic embryogenesis, immature cones of 65 elite families of loblolly pine were selected. The germoplasm used for field test was composed of 238 clones from 31 families, propagated via somatic embryogenesis. Genetic and statistical analyses were performed by estimation of variance components via residual maximum likelihood (Reml) and prediction of breeding values via best linear unbiased prediction (Blup) using the Selegen-Reml/Blup software. According to the results, genetic variability detected enables high possibility of genetic gain by selection among families, for both characters presence of somatic embryos and number of clones by families of Pinus taeda designed to somatic embryogenesis. There is little or no genetic correlation between the number of clones propagated via somatic embryogenesis and characteristics height, diameter, volume assessed and survival at four years of age in clonal tests. We conclude that there is greater genetic gain the ability to spread by somatic embryogenesis by selecting families of Pinus taeda.

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“…Later, the effect of parent genotype on somatic embryogenesis in P. sylvestris was described. No mother × father interaction was found, indicating that mothers successful at initiation and after 6 months in tissue culture, pollinated by any of the successful fathers, produced somatic lines and mature somatic embryos [10]. Enhancement of somatic embryogenesis from immature zygotic embryos of P. sylvestris and with optimized culture conditions and tight quality control, over 90% of the embryos germinate and develop into vigorously growing emblings [11].…”

mentioning

confidence: 95%

Imaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM

2015

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Environmental scanning electron microscopy enables the investigation of uncoated pine early embryogenic tissue samples in situ. The samples were examined under low vacuum conditions (air pressure 550 Pa) at a temperature of around -18°C by the AQUASEM II noncommercial environmental scanning electron microscope. The native extracellular matrix surface network was imaged by the environmental scanning electron microscope and in dark field mode of the optical microscope too. The backscattered electron detector disclosed brightness loci in the cells of early embryogenic culture. This work shows images of native pine embryogenic tissues. The continuity of extracellular matrix with structural integrity of plant organism is discussed.

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Effect of parent genotype on somatic embryogenesis in Scots pine (Pinus sylvestris)

Cited by 69 publications

References 28 publications

The effect of culture medium formulation on Pinus nigra somatic embryogenesis

The effect of culture medium formulation on Pinus nigra somatic embryogenesis

PARÂMETROS GENÉTICOS PARA A CAPACIDADE DE PROPAGAÇÃO DE Pinus taeda POR EMBRIOGÊNESE SOMÁTICA1

Imaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM

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