Effect of Pamamycin-607 on Secondary Metabolite Production byStreptomycesspp.

Mycobacterium ulcerans toxin, mycolactone may enhance host‐seeking and oviposition behaviour by Aedes aegypti (L.) (Diptera: Culicidae)

“…The fact that MU is an environmental pathogen and does not depend on the human host for survival suggests that mycolactone has an important role in the population biology and community ecology of the species (Marsollier et al ., ). As previously stated, the macrolide structure of mycolactone suggests the possibility that the molecule may be an antagonist to bacteria with QS machinery or may serve as a regulator of secondary metabolism (Hashimoto et al ., ; Romero et al ., ), and work is currently underway to understand QS related, mycolactone mechanisms. Taken together, our data suggest that mycolactone may increase MU fitness under conditions where mycolactone functions as a mosquito attractant.…”

“…Furthermore, QS and interkingdom interactions has been observed in interactions between bacteria and mosquitoes (Lowery et al, 2008;Ezenwa et al, 2012;Ezenwa and Williams, 2014), where mosquitoes 'eavesdrop' on the communication of bacteria, that subsequently influence mosquito decision-making and behavioural responses (Ponnusamy et al, 2010;Verhulst et al, 2010a). The macrolide structure of mycolactone suggests the possibility that the molecule may be an antagonist to bacteria with quorum sensing machinery (similar to acyl homoserine lactones) or may serve as a regulator of secondary metabolism (Hashimoto et al, 2011;Romero et al, 2011). However, QS compounds produced by MU and their possible influence on arthropod behaviour, particularly mosquito attraction and oviposition, are unknown.…”

Studies on bioactive natural products involved in the growth and morphological differentiation of microorganisms

“…12) Quantitative analysis of the effect showed that pamamycin-607 at 6.6 µM increased antibiotic production by 1.5 to 2.7 times. 15) These results suggest the application of pamamycin-607 would be useful for the screening of novel substances whose production is induced by pamamycin-607 and for improving the production of useful secondary metabolites with low productivity.…”

Mycobacterium tuberculosis puromycin hydrolase displays a prolyl oligopeptidase fold and an acyl aminopeptidase activity

“…Puromycin hydrolysis characterization was done by mixing 200 μg/mL puromycin in 0.1 mM Tris 150 mM NaCl 10% glycerol pH 7.8 and MtPMH at a final concentration of 70 μg/mL, incubating overnight at 30 C. HPLC analysis of the puromycin hydrolysis was adapted from reference 20 and was performed with an Agilent 1260 HPLC using Agilent Eclipse plus C18, 4.6 × 100 mm column with 5 μm pore. 20 The mobile phase consisted of CH 3 CN 0.1% aq NH 4 OAC (25:75) solvent.…”