Effect of palmitate on hepatic biosynthetic functions at hyperthermic temperatures

Collins, Fred G.; Mitros, Frank A.; Skibba, Joseph L.

doi:10.1016/0026-0495(80)90077-3

Cited by 37 publications

(27 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under the same conditions, gluconeogcnsis at 43 °C was significantly lower than that found at 37-42 °C [3,21]. These data suggest increased diversion of C3 intermediates to oxidation at 43 °C.…”

Section: Discussionsupporting

confidence: 58%

“…This conclusion is supported by the fact that addition of exogenous palmitate. a source of acetyl-CoA, attenuated these ef fects of hyperthermia on the rat liver [3,21]. Thus, accumulation of perfusate lactate in the present studies with Fluosol-43 could re sult from impaired gluconeogenesis as well as impaired pyruvate oxidation.…”

Section: Discussionmentioning

confidence: 63%

“…Hyperlactatemia which developed in the perfusate could result from impaired trans port of reducing equivalents from cytosol to mitochondria, decreased activity of the pyru vate dehydrogenase complex, NAD deple tion, or impaired gluconeogenesis from lac tate [1], In perfused livers from starved rats, exogenous lactate utilization was increased at hyperthermic temperatures up through 42 °C, but not at 43 °C [3,21]. Under the same conditions, gluconeogcnsis at 43 °C was significantly lower than that found at 37-42 °C [3,21].…”

Section: Discussionmentioning

confidence: 99%

“…This same technique allowed perfusion of the canine liver at hyperthermic temperatures with preservation of hepatic function and dog survival [22]. Isolation perfusion of the liver with chemotherapeutic agents and/or heated perfusate were suggested as potential thera peutic modalities for treating hepatic neo plasms [2,11,17,20,22], Hyperthermic liver perfusion results in increased metabolic demands with an increase in oxygen con sumption [3,21].…”

mentioning

confidence: 99%

See 3 more Smart Citations

Canine Liver Isolation-Perfusion at Normo- and Hyperthermic Temperatures with Perfluorochemical Emulsion (Fluosol-43)

Skibba¹,

Sonsalla²,

Petroff³

et al. 1985

Eur Surg Res

View full text Add to dashboard Cite

A perfluorocarbon emulsion, Fluosol-43, was used as a blood substitute for oxygen transport during isolation-perfusion of the dog liver at 37 and 43 °C. Preservation of hepatic functional integrity was assessed through analysis of perfusate constituents and animal survival after perfusion. Flow to the liver during perfusion was > 1 ml/min/g with one-third of total flow provided through the hepatic artery and two-thirds through the protal vein. Perfusion duration was 3 h. The pO₂ gradient across the liver indicated that oxygen was consumed during perfusion at both temperatures. The expected rise in pCO₂ and decrease in pH of the outflow perfusate is consistent with active aerobic metabolism. Perfusate chemistries lactate, pyruvate, glucose, urea, total α-amino acids, ketone bodies and SGPT demonstrated that hepatic functional integrity was maintained during perfusion. Significant differences (p < 0.05) between temperatures occurred in the perfusate levels of lactate, pyruvate, L/P ratios, glucose and total α-amino acids. Animal survival after a 3-hour perfusion was 3/4 at 37 °C, and 2/5 at 43 °C. After perfusion, SGPT levels were significantly higher in dogs subjected to perfusion at 43 °C. The success of these experiments demonstrates that perfusion of the liver with Fluosol-43 was not in itself hepatotoxic, and that Fluosol-43 may allow perfusion of the liver at 43 °C with only mild toxicity.

show abstract

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Canine Liver Isolation-Perfusion at Normo- and Hyperthermic Temperatures with Perfluorochemical Emulsion (Fluosol-43)

Skibba¹,

Sonsalla²,

Petroff³

et al. 1985

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…Hyperthermia has signif icant potential for the treatment of multifocal primary or secondary malignant liver tumors [23] for which standard therapies have been disappointing. However, little is known about the toxicity of hyperthermia to the liver: ex perimental studies [1,2,6,18,22] and clinical observations using total-body hyperthermia [14] have underlined the risk of injury to liver parenchymal cells, but the relation be tween the degree of hyperthermia and the extent of hepatocellular injury is not clear.In the rat, the thinness of the liver permits poorly penetrating infrared radiation to be used for the study of liver hyperthermia. The purpose of this experimental study in the rat was to precisely define the thermosensitivity of the normal liver and of the neoplastic liver to infrared hyperthermia and the conditions required for using hyperthermia as a treat ment for experimental malignant liver tu mors in the rat.…”

mentioning

confidence: 99%

Effects of Hyperthermia on Normal or Neoplastic Rat Liver

Adam¹,

Poggi²,

Houssin³

et al. 1985

Eur Surg Res

View full text Add to dashboard Cite

An experimental study was conducted in rats to evaluate the sensitivity of the liver to infrared hyperthermia. A 15-min hyperthermia session treating only the liver was done in rats with a normal hepatic parenchyma and in rats with hepatocarcinoma induced by chronic 3’-diethylaminoazobenzene intoxication, at various ranges of intrahepatic temperature. In normal rats, 40–42 °C hyperthermia was well tolerated, but the mortality rate increased when the intrahepatic temperature exceeded 42 °C. In rats with tumors, a 40–42 °C hyperthermia session was well tolerated in case of small tumors, but resulted in a high mortality rate in case of large tumors. In all cases, death occurred as a consequence of liver injury. This study using a simple method of hyperthermia defines the thermosensitivity of the neoplastic or normal rat liver and provides a basis for further investigations on the effect of hyperthermia on experimental liver tumors.

show abstract

Effects of systemic hyperthermia and intrahepatic infusion with 5-fluorouracil

Daly

Smith²,

Frazier

et al. 1982

Cancer

View full text Add to dashboard Cite

Potential hepatotoxicity from systemic hyperthermia (43 degrees C) +/- simultaneous hepatic artery infusion with 5-FU was evaluated in an animal model. Twenty-two dogs had aorta-vena caval shunts (8 mm Dacron grafts) placed, and 10 of these dogs had silastic catheters inserted in their hepatic arteries. Two weeks later, Group I (n = 8) was heated to 43 degrees C for one hour (distal esophageal + intrahepatic temperature) using the shunts and blood-heat exchangers; Group II (n = 6) was heated to 43 degrees C for one hour with simultaneous intrahepatic infusion of 5-FU (10 mg/kg); Group III (n = 8) was shamheated (37 degrees C) and underwent a one hour intrahepatic infusion with 5-FU (10 mg/kg). Serum alkaline phosphatase, SGOT, SPGT (IU/ml) and bilirubin were measured, and liver biopsies were obtained at 0 and 1 hour, at one and seven days. Mean SGOT levels increased significantly (P less than 0.05) in Group II from 19 +/- 2 to 31 +/- 6 and 63 +/- 18 at one hour and one day; these levels rose slightly in Group I from 31 +/- 5 to 40 +/- 8 and 47 +/- 8 at one hour and one day. Hepatocellular enzyme levels returned to normal at seven days in both groups. Mean SGOT and SGPT levels remained similar in Group III at all time periods. No significant differences in mean serum alkaline phosphatase or bilirubin levels were noted. There was no histologic evidence of hepatocellular necrosis at any time period. Survival was 6/8, 5/6 and 8/8 dogs in Groups I, II, and III, respectively. Systemic hyperthermia to 43 degrees C for one hour in dogs does not adversely affect serum hepatic enzymes or cell structure; reversible serum hepatic enzyme changes occurred when hyperthermia was combined with hepatic artery infusion with 5-FU.

show abstract

Effect of palmitate on hepatic biosynthetic functions at hyperthermic temperatures

Cited by 37 publications

References 32 publications

Canine Liver Isolation-Perfusion at Normo- and Hyperthermic Temperatures with Perfluorochemical Emulsion (Fluosol-43)

Canine Liver Isolation-Perfusion at Normo- and Hyperthermic Temperatures with Perfluorochemical Emulsion (Fluosol-43)

Effects of Hyperthermia on Normal or Neoplastic Rat Liver

Effects of systemic hyperthermia and intrahepatic infusion with 5-fluorouracil

Contact Info

Product

Resources

About