Effect of Paeotang on tumor necrosis factor α-induced vascular inflammation in human umbilical vein endothelial cells

Kim, Dae Hwan; Lee, So Min; Lee, Yun Jung; Yoon, Jung Joo; Yu, Yun Cho; Kang, Dae Gill; Lee, Ho Sub

doi:10.1007/s11655-017-2759-3

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…2 b–d). Consistent with our study, the downregulation of TNF- α and IL1 β lead to the decline of Selectin-E gene expression [ 32 – 35 ]. In addition, neutrophil infiltration mainly appeared at day 3 after detorsion and then began to fall at day 7 and day 15 after detorsion evidenced by the expression of MPO was highest at day 3 after testis detorsion and became low again at day 7 and day 15(Fig.…”

Section: Discussionsupporting

confidence: 90%

Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

et al. 2020

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Background This study was designed to determine the effect of human umbilical cord multipotent mesenchymal stromal cells (hUC-MSC) on acute ischemia/reperfusion (I/R) injury of spermatogenic cells. Method The testicular I/R rat model was established through 720° torsion for 1 h. hUC-MSC were intravenously injected 10 min before detorsion. Injury severity of spermatogenic cells was estimated by Johnsen’s score. The proliferating of recipient spermatogonia was measured by the immunostaining of antibodies against Ki67, and all germ cells were detected with DDX4 antibody. And recipient spermatogenesis was assessed by staining spermatozoa with lectin PNA. The levels of inflammatory factors were measured by real-time PCR. And the Selectin-E expression, neutrophil infiltration in the testes was detected by immunostaining. Germ cells apoptosis was tested by TUNEL assay and western blot. Furthermore, the oxidative stress was tested by reactive oxidative species (ROS) levels. In vitro, the condition medium (CM) of hUC-MSC was used to culture human umbilical vein endothelial cells (HUVECs), so as to assess the paracrine effect of hUC-MSC on HUVECs. The protein chip was used to measure the relative concentration of the secretory proteins in the CM of hUC-MSC. Result hUC-MSC greatly alleviated the testicular injury induced by testis I/R. The levels of proinflammatory factors were downregulated by hUC-MSC in vivo and in vitro. Neutrophil infiltration, ROS, and germ cell apoptosis in testicular tissues were greatly reduced in the group of hUC-MSC. Paracrine factors secreted by hUC-MSC including growth factors, cytokines, and anti-inflammatory cytokine were rich. Conclusion This study demonstrated that intravenously injected hUC-MSC could protect the spermatogenic cells against I/R injury by reducing the inflammatory response, apoptosis, and acute oxidative injury. Paracrine mechanism of hUC-MSC may contribute to the protection of spermatogenic cells against I/R injury. Therefore, the present study provides a method for clinical treatment of attenuate I/R injury of spermatogenic cells.

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Section: Discussionsupporting

confidence: 90%

Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

et al. 2020

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“…In addition, physical parts of Prunus persica are used as important ingredients in traditional Chinese medicine prescriptions. Prunus persica has been included in the traditional Chinese medicine prescription to treat blood stasis [ 6 ]. Fukuda et al reported that several isolated glycosides from Prunus persica seeds can inhibit in vitro and in vivo tumorigenesis [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Ethanol Extract of Root of Prunus persica Inhibited the Growth of Liver Cancer Cell HepG2 by Inducing Cell Cycle Arrest and Migration Suppression

Shen

Wang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Liver cancer is the second most lethal cancer and hepatocellular carcinoma (HCC) is the primary cancer subgroup. However, the current chemotherapy agents remain ineffective and present wide side effects for advanced HCC patient. In this study, we investigated the antitumor role of ethanol extract of root of peach tree (Prunus persica (L.) Batsch and hereafter designated as TSG in short of its Chinese name), which is an important ingredient in Chinese medicine prescription, in liver cancer cell HepG2. By cell viability assay, we showed that addition of TSG in the culture medium inhibited the cell growth of HepG2 cells in a dose and time-dependent way. Cell cycle analysis indicated that TSG caused sustained M/G2 phase arrest. The expression of mitosis-related protein Cdc25c was impaired upon TSG treatment. Furthermore, wound healing assay demonstrated that TSG treatment notably suppressed the migration of HepG2 cells and the expression of extracellular matrix metalloprotease, MMP3 and MMP9. Most significantly, administration of TSG inhibited in vivo tumor growth in nude mice. Our findings suggested that TSG may serve as a source to isolate anti-HCC therapeutic ingredients.

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“…Furthermore, the expression level of ror2 was the highest following treatment with 10 ng/ml TnF-α. Therefore, 10 ng/ml was selected as the concentration of TnF-α to be used in the following experiments based on the present and previous results (22,25,26). To further investigate the effects of ror2 on TnF-α-induced vascular endothelial cell injury, two shrna expression vectors, shrna-ror2-1 and shrna-ror2-2, were used to knock down the expression of ror2 in HuVecs, and cells transfected with empty plasmids were used as a negative control.…”

Section: Resultsmentioning

confidence: 99%

“…additionally, the expression levels of icaM-1 and VCAM-1 were significantly enhanced by TNF-α stimulation. icaM-1 and VcaM-1 are adhesion molecules that can bind to monocytes and lymphocytes and cause adhesion reactions (25). McP-1 can promote the transformation of monocytes into macrophages, leading to the formation of foam cells (40,41).…”

Section: Discussionmentioning

confidence: 99%

Knockdown of Ror2 suppresses TNF‑α‑induced inflammation and apoptosis in vascular endothelial cells

et al. 2020

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Wnt family member 5a (Wnt5a) is a noncanonical member of the Wnt family that is highly expressed in atherosclerosis. Studies have shown that Wnt5a/receptor tyrosine kinase-like orphan receptors 2 (ror2) signaling can participate in the formation of foam cells; however, the role of ror2 in vascular endothelial cells during atherosclerotic injury is unknown. Therefore, the present study aimed to investigate the role of ror2 in tumor necrosis factor (TnF)-α-induced vascular endothelial cell injury and investigate whether it could be regulated by Wnt5a. Human umbilical vein endothelial cells were transfected with short hairpin RNA specific against ror2 in the absence or presence of TnF-α. The alteration of inflammatory cytokine levels was detected, and the expression of adhesion molecules was assessed. Western blot and flow cytometry analyses were used to detect the activation of nuclear factor-κB (nF-κB) signaling and cell apoptosis. The interaction between ror2 and Wnt5a was confirmed by immunoprecipitation. ror2 was upregulated upon TnF-α stimulation. Knockdown of ror2 inhibited the TnF-α-induced release of inflammatory cytokines, the expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 and the activation of nF-κB signaling. Furthermore, cell apoptosis induced by TnF-α was rescued by ror2 silencing. in addition, Wnt5a expression was increased by TnF-α, and ror2 could bind to Wnt5a, the knockdown of which could downregulate the levels of ror2. in conclusion, it was demonstrated that ror2 was upregulated upon TnF-α stimuli, and interference of ror2 regulated by Wnt5a could suppress TnF-α-induced inflammation and apoptosis in vascular endothelial cells.

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Effect of Paeotang on tumor necrosis factor α-induced vascular inflammation in human umbilical vein endothelial cells

Abstract: These data suggest that Paeotang might be a benefificial therapeutic in vascular inflflammation through regulation of Nrf2-mediated HO-1 expression and inhibition of ROS/NF-κB signaling pathway. Thus, Paeotang maybe serve as a potential anti-atherogenic agent.

Cited by 7 publications

References 32 publications

Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

Human umbilical cord multipotent mesenchymal stromal cells alleviate acute ischemia-reperfusion injury of spermatogenic cells via reducing inflammatory response and oxidative stress

Ethanol Extract of Root of Prunus persica Inhibited the Growth of Liver Cancer Cell HepG2 by Inducing Cell Cycle Arrest and Migration Suppression

Knockdown of Ror2 suppresses TNF‑α‑induced inflammation and apoptosis in vascular endothelial cells

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