Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid

Olaifa, Folashade Helen; Ayo, Joseph Olusegun; Ambali, Suleiman Folorunsho; Rekwot, P.I.

doi:10.4102/ojvr.v79i1.413

Cited by 14 publications

(9 citation statements)

References 24 publications

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“…For instance, the initiation of pulmonary respiration causes changes in blood pH that could explain the higher osmotic fragility of the foal's erythrocytes. This is in accordance with previous studies carried out on donkeys, domestic fowl, cattle, pigs, rats, and rabbits that found a significant influence of blood pH on EOF. Some authors have concluded that the decrease in pH accelerates EOF by affecting the potassium–chloride co‐transporter on the red cell membrane in vitro.…”

Section: Discussionsupporting

confidence: 93%

“…Foals had a significantly higher EOF than mares at NaCl concentrantions of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5% from day 1 until day 18. The EOF in hypotonic solution has been studied in artiodactyls, humans, donkeys, camels, pigs, and horses affected by immune‐mediated hemolytic anemia . It has been shown that EOF is related to the morphologic characteristics and, particularly, to the size of the erythrocytes and their cell membrane integrity .…”

Section: Discussionmentioning

confidence: 99%

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Erythrocyte osmotic fragility and select hematologic variables in postparturient mares and their foals

Arfuso

Quartuccio

Bazzano

et al. 2016

Veterinary Clinical Pathol

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Erythrocyte osmotic fragility and select hematologic variables in postparturient mares and their foals

Arfuso

Quartuccio

Bazzano

et al. 2016

Veterinary Clinical Pathol

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“…Oxidative stress is thought to aggravate the symptoms of many diseases, including haemolytic anaemias, because erythrocytes are highly susceptible to oxidative damage induced by free radicals or reactive oxygen species [ 40 , 41 ]. Studies in goats, pigs and donkeys showed that stressful conditions such as transportation and packing induced a significant increase in the erythrocyte OF that was not present when the animals were treated with an anti-oxidant (ascorbic acid) before undergoing the stressful event, suggesting that the increased OF was a consequence of oxidative stress [ 41 - 43 ]. Although, not yet studied, oxidative stress may have similar effects on canine erythrocytes and may contribute to the increased OF that is seen in dogs with IMHA.…”

Section: Discussionmentioning

confidence: 99%

The use of the rapid osmotic fragility test as an additional test to diagnose canine immune-mediated haemolytic anaemia

et al. 2013

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BackgroundDiagnosing canine immune-mediated haemolytic anaemia (IMHA) is often challenging because all currently available tests have their limitations. Dogs with IMHA often have an increased erythrocyte osmotic fragility (OF), a characteristic that is sometimes used in the diagnosis of IMHA. Since the classic osmotic fragility test (COFT) is time-consuming and requires specialized equipment, an easy and less labour-intensive rapid osmotic fragility test (ROFT) has been used in some countries, but its diagnostic value has not yet been investigated.This study aimed to evaluate erythrocyte osmotic fragility in dogs with and without IMHA, to compare results of the classic (COFT) and rapid (ROFT) test and to assess the value of the ROFT as diagnostic test for canine IMHA.Nineteen dogs with IMHA (group 1a), 21 anaemic dogs without IMHA (group 1b), 8 dogs with microcytosis (group 2), 13 hyperlipemic dogs (group 3), 10 dogs with lymphoma (group 4), 8 dogs with an infection (group 5) and 13 healthy dogs (group 6) were included.In all dogs, blood smear examination, in-saline auto-agglutination test, Coombs’ test, COFT and ROFT were performed. In the COFT, OF5, OF50 and OF90 were defined as the NaCl concentrations at which respectively 5, 50 and 90% of erythrocytes were haemolysed.ResultsCompared with healthy dogs, OF5 and OF50 were significantly higher in group 1a (P < 0.001) and OF5 was significantly higher in group 3 (P = 0.0266). The ROFT was positive in 17 dogs with IMHA, 10 hyperlipemic dogs, one anaemic dog without IMHA and one healthy dog.ConclusionsOsmotic fragility was increased in the majority of dogs with IMHA and in dogs with hyperlipidemia, but not in dogs with microcytosis, lymphoma or an infection. Although more detailed information was obtained about the osmotic fragility by using the COFT, the COFT and ROFT gave similar results. The ROFT does not require specialized equipment, is rapid and easy to perform and can be used easily in daily practice. Although, the ROFT cannot replace other diagnostic tests, it may be a valuable additional tool to diagnose canine IMHA.

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“…The erythrocytes therefore, become more fluid and allow more movement and permeability of the cells, thereby damaging them. Furthermore, high temperatures recorded during transportation may cause proteins of the erythrocytes membrane to denature, which may lead to membrane disintegration and haemolysis [7,10,25]. Thirdly, transport-induced oxidative stress on erythrocytes due to the adverse effect of toxic ROS, presumably generated during the transportation, may also contribute to the deterioration of erythrocyte properties; leading to increase in haemolysis.This is true because the erythrocytes are susceptible to oxidative stress as a result of high contents of unsaturated fatty acids in their membranes [1].…”

Section: Citationmentioning

confidence: 99%

“…The EOF may be employed as a biomarker and additional diagnostic tool for the assessment of transport-induce oxidative stress in quails. [7,10].The use of EOF as a biomarker of road transport-induced stress in livestock is a new field of study that may add to the arsenals of tests, currently used in the diagnosis of stress due to road transportation [11][12][13]. Body temperature measurement is an important method of evaluation of physiological status.…”

Section: Introductionmentioning

confidence: 99%

Effects of Antioxidants Vitamin E and C on ErythrocyteFragility, HaemoglobinIndex and Colonic Temperature of Transported Japanese Quails (Coturnixcoturnix japonica)

Minka¹

2013

J Veterinar Sci Technolo

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The present study investigated the effects of antioxidants Vitamin E (VE) and C (ascorbic acid, AA) on erythrocytes osmotic fragility (EOF), haemoglobin index (HI), colonic temperature (CT) and recovery times of transported Japanese quails during the hot-dry conditions. Two hundred male Japanese quails were used. The birds were randomly and equally divided into four groups: VE and AA groups, administered with 0.5 mg/kg and 200 mg/ kg body weight of VE and AA, respectively, control transported group (control group), administered with 0.5 ml of sterile water, and sedentary non-transported group (S). One hour after the administration of the antioxidants, the birds were transported by road for 2 h. The EOF, HI and CT were recorded before transportation, immediately after transportation (0) and on 3 rd and 7 th day of post-transportation period. The results showed that transportation induced hyperthermia, increased EOF and hypochromic erythrocytes in the quails, the effects of which were mitigated by antioxidants VE and AA. Base-line values for EOF were returned to normal in the control, AA and VE quails on days 7, 3 and 0, respectively post-transportation. The findings showed that recovery time after transportation in quails should be 7 days; but the duration may be reduced to 3 days if treated with AA or eliminated completely if the quails are treated with VE before transportation. The ameliorating effect was more pronounced in quails administered with antioxidant VE. The EOF may be employed as a biomarker and additional diagnostic tool for the assessment of transport-induce oxidative stress in quails. [7,10].The use of EOF as a biomarker of road transport-induced stress in livestock is a new field of study that may add to the arsenals of tests, currently used in the diagnosis of stress due to road transportation [11][12][13]. Body temperature measurement is an important method of evaluation of physiological status. It is also an ideal indicator for the assessment of stress and adaptability of livestock, including poultry, to various environmental stress factors [14][15][16]. Effects of Antioxidants Vitamin E and C on ErythrocyteFragilitySeveral stress factors have been reported to decrease the level of body antioxidants, vitamin E (VE) and vitamin C, otherwise known as ascorbic acid (AA); resulting in increases in the body requirements in these vitamins [14,[17][18][19]. The administration of the vitamins exerts ameliorating effects which are well manifested; when the body AA and VE are either overwhelmed or exhausted as a result of increased ROS generation by stress factors that overtax the animal control systems [18,20]. Therefore, a decrease in the concentration of antioxidant vitamins causes an increase in lipid peroxidation in the plasma and tissues, leading to damage of cell membranes [21,22]. Previous reports have revealed the beneficial effects of supplementation of VE and AA on birds and animals, subjected to various oxidative stress factors [12,16,18,22].Several studies have evaluated the respon...

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Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid

Cited by 14 publications

References 24 publications

Erythrocyte osmotic fragility and select hematologic variables in postparturient mares and their foals

Erythrocyte osmotic fragility and select hematologic variables in postparturient mares and their foals

The use of the rapid osmotic fragility test as an additional test to diagnose canine immune-mediated haemolytic anaemia

Effects of Antioxidants Vitamin E and C on ErythrocyteFragility, HaemoglobinIndex and Colonic Temperature of Transported Japanese Quails (Coturnixcoturnix japonica)

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