Effect of P15E related retroviral material isolated from head and neck carcinomas on delayed type hypersensitivity skin reactions in mice

Ib, Tan; Ha, Drexhage; Rj, Scheper; Bm, von Blomberg-van de Flier; M, dP Haan-Meulman; Gb, Snow; Fj, Balm

doi:10.1288/00005537-198706000-00012

The Laryngoscope

1987

DOI: 10.1288/00005537-198706000-00012

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Effect of P15E related retroviral material isolated from head and neck carcinomas on delayed type hypersensitivity skin reactions in mice

Tan Ib¹,

Drexhage Ha²,

Scheper Rj³

et al.

Abstract: Low molecular weight factors, derived from head and neck carcinomas (H/N ca LMWFs), exert a serious inhibitory effect on monocyte chemotaxis both in vivo and in vitro. This effect can be neutralized by the treatment of the factors with three different monoclonal antibodies to P15E, one of the structural envelope proteins of murine leukemia virus (MuLV). In this study, we report the effect of the H/N ca LMWFs on the delayed type hypersensitivity responsiveness in mice: the factors significantly inhibited the 24… Show more

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1992

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Cited by 4 publications

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Synthetic hexapeptides derived from the transmembrane envelope proteins of retroviruses suppress N-formylpeptide–induced monocyte polarization

Oostendorp

Schaaper²,

Post³

et al. 1992

Journal of Leukocyte Biology

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Retroviral infections are frequently associated with immunosuppression. Retroviral transmembrane envelope proteins (TM proteins) play an important role in this phenomenon. CKS-17, a synthetic heptadecapeptide, represents the immunosuppressive site of these retroviral TM proteins. Here we support on the further delineation of this immunosuppressive site using CKS-17-derived hexapeptides. The N-formyl-methionyl-leucyl-phenylalanine-induced monocyte polarization assay was used throughout this study because this monocyte function has been shown to be highly sensitive to TM protein p15E-related immunosuppression. We found that in addition to CKS-17 one CKS-17-derived hexapeptide, LDLLFL, reversibly inhibited monocyte polarization, with 50% inhibitory concentrations of 20 and 2 microM respectively. LDLLFL-mediated inhibition was sequence specific because the reverse peptide LFLLDL and scrambled peptides were not inhibitory. Hexapeptides corresponding to LDLLFL, but derived from various retroviruses other than murine leukemia virus, also inhibited monocyte polarization. Peptides most homologous to LDLLFL-LDILFL (feline leukemia virus) and LDLLFW (human T lymphotropic virus types I and II)--were the most potent inhibitors. Peptides homologous to primate and human endogenous proviruses were not suppressive. LDLLFL and some of its homologous also inhibited polarization of neutrophilic granulocytes. These findings lend further support to the view that conserved retroviral TM protein-related peptides can play an important role in suppression of inflammatory cell function as encountered in retrovirus-associated immunosuppression.

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Synthetic hexapeptides derived from the transmembrane envelope proteins of retroviruses suppress N-formylpeptide–induced monocyte polarization

Oostendorp

Schaaper²,

Post³

et al. 1992

Journal of Leukocyte Biology

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Immunosuppression by retroviral-envelope-related proteins, and their role in non-retroviral human disease

Oostendorp

Meijer

Scheper

1993

Critical Reviews in Oncology/Hematology

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The presence of immunosuppressive ‘p15E-like’ factors in the serum and urine of patients suffering from malign and benign breast tumours

Stöger

Wilders‐Truschnig

Samonigg

et al. 1993

Clinical and Experimental Immunology

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SUMMARYCertain types of tumours are capable of producing factors inhibiting mononuclear phagocyte chemotaxis which may contribute to defects in immunosurveillance. In head and neck cancer these factors are said to be related to the retroviral protein pi 5H, This study examines thepresenceof pi 5E-like factors in serum and urine of patients with malign and benign breast tumours. Thirty patients with breast cancer. 29 patients with benign breast masses, and 2H healthy controls were tested blindly with the monocyie polarization assay, using N-formyl-methionyl-leucylphenylalanine as chenioattractant. The low molecular weight fractions prepared of sera oi the malign tumour patients inhibited the monocyte polarization significantly (mean inhibition M'V.., s.d,= 12) compared with those of benign tumour patients (15'^, s.d, = 7) and of controls (14'Ki, s.d,=6). The observed inhibitory efTeets on the monoeyte polarization could be eompensated by MoAbs reactive to pl5E-rclaicd anUgcns, The mean diftercnce belween ihc polari/alion inhibilion wilh and without anvi-pl 5E adsorption (the'pi 5E-like factor-induced inhibition') was 25' K> (s.d. = 13) in lhe breast cancer group, compared with 7' !^i, (s.d, = 5) in the benign tumour patients and 5"';, (s,d. = 4) in the healthy control group. Surgical removal of the tumours resulted in a restoration of the monocyie polarization in 20/ 23 (87'^) patients ofthe breast cancer group. Results testing preopcrative urine samples correlated well with those of corresponding sera, The.scdata give additional support to the concept that tumourderived pl5E-iike factors are responsible for the inhibitory elTect on monocyte chemotaxis in breast cancer patients, and that these factors can be found in serum as well as in urine.

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Effect of P15E related retroviral material isolated from head and neck carcinomas on delayed type hypersensitivity skin reactions in mice

Cited by 4 publications

References 14 publications

Synthetic hexapeptides derived from the transmembrane envelope proteins of retroviruses suppress N-formylpeptide–induced monocyte polarization

Synthetic hexapeptides derived from the transmembrane envelope proteins of retroviruses suppress N-formylpeptide–induced monocyte polarization

Immunosuppression by retroviral-envelope-related proteins, and their role in non-retroviral human disease

The presence of immunosuppressive ‘p15E-like’ factors in the serum and urine of patients suffering from malign and benign breast tumours

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