Effect of ozone and nitrogen dioxide on the permeability of bronchial epithelial cell cultures of non‐asthmatic and asthmatic subjects

Bayram, Hasan; Rusznák, C.; Khair, Omar A.; Sapsford, RJ; Abdelaziz, Muntasir M.

doi:10.1046/j.1365-2745.2002.01435.x

Cited by 60 publications

(43 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such changes have been seen to correspond to an increase in ionic and macromolecular permeability (Xiao et al, 2011;Bai et al, 2015;Freishtat et al, 2011;Watson et al, 2010). It has also been shown that the permeability of the bronchial epithelial cell layer is only increased in asthmatic subjects after exposure to ozone or nitrogen dioxide (Bayram et al, 2002), and that mediator release is altered after exposure of ALI cultures from asthmatic donors to respiratory syncytial virus (RSV) or particulate matter (Hackett et al, 2011b). Using cells derived from patients with COPD, ALI cultures show higher baseline levels of cytokine expression and increased susceptibility to RSV infection, despite an increased IFN response tem is specially designed for direct contact between cells and components of the test atmosphere at the air/liquid interface.…”

Section: Fully Differentiated 3d Human Airway Epithelial Modelsmentioning

confidence: 97%

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

Gordon

Daneshian

Bouwstra

et al. 2015

ALTEX

122

103

View full text Add to dashboard Cite

SummaryModels of the outer epithelia of the human body -namely the skin, the intestine and the lung -have found valid applications in both research and industrial settings as attractive alternatives to animal testing. A variety of approaches to model these barriers are currently employed in such fields, ranging from the utilization of ex vivo tissue to reconstructed in vitro models, and further to chip-based technologies, synthetic membrane systems and, of increasing current interest, in silico modeling approaches. An international group of experts in the field of epithelial barriers was convened from academia, industry and regulatory bodies to present both the current state of the art of non-animal models of the skin, intestinal and pulmonary barriers in their various fields of application, and to discuss research-based, industry-driven and regulatory-relevant future directions for both the development of new models and the refinement of existing test methods. Issues of model relevance and preference, validation and standardization, acceptance, and the need for simplicity versus complexity were focal themes of the discussions. The outcomes of workshop presentations and discussions, in relation to both current status and future directions in the utilization and development of epithelial barrier models, are presented by the attending experts in the current report.

show abstract

Section: Fully Differentiated 3d Human Airway Epithelial Modelsmentioning

confidence: 97%

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

Gordon

Daneshian

Bouwstra

et al. 2015

ALTEX

122

103

View full text Add to dashboard Cite

show abstract

“…60 Using animal or in vitro models, NO 2 produces eosinophilic inflammation, enhances epithelial damage, reduces mucin expression and increases baseline smooth muscle tone. [60][61][62][63] Repeated exposure to high doses of NO 2 is associated with increased breath frequency and decreased lung distensibility and gas exchange. 64 It has also been explained that NO 2 decreases bactericidal activity and alveolar macrophage activity.…”

Section: Mechanismsmentioning

confidence: 99%

The Role of Air Pollution in Adult-Onset Asthma: A Review of the Current Evidence

Jacquemin

Schikowski

Carsin

et al. 2012

Semin Respir Crit Care Med

View full text Add to dashboard Cite

The causes of adult-onset asthma are poorly established, and the asthmogenic role of air pollution has been investigated primarily in children. This review assesses the current evidence of the association between air pollution and asthma incidence among subjects free of asthma at least until late childhood. Seven publications from five study populations fulfilled the inclusion criteria (one case-control and six cohort studies). All but one used markers of local traffic-related air pollution to characterize long-term exposure. Those studies reported similar associations with traffic-related air pollution. However, protocols, definitions of asthma, and exposure assignment were rather heterogeneous, and three publications relied on the same study; thus we abstain from meta-analytic summaries. Reported patterns of effect modification (e.g., by sex, atopy, or smoking) were inconsistent. Overall, the role of traffic-related air pollution in adult-onset asthma is less conclusive than in childhood asthma. Larger studies with more consistent definitions of phenotypes and exposure assessment for local traffic-related pollutants (e.g., ultrafine particles) are needed. Pooling existing cohorts such as in the ongoing European ESCAPE and TRANSPHORM consortia are promising steps. There is, however, a need for large-scale megacohorts to investigate these effects in standardized ways and to identify the most susceptible populations.

show abstract

“…For example, TNF-α has been shown to alter epithelial cell permeability, as well as to stimulate IL-6, IL-8 and GM-CSF expression [3]. A number of studies have shown that the epithelium of patients who have airway inflammatory diseases is structurally and functionally altered [4][5][6][7], and that bronchial epithelial cells that are isolated from patients with asthma or cystic fibrosis (CF) express increased levels of cytokines [1,3]. Therefore, it is important to fully understand gene regulation in airway epithelium in order to alleviate airway inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

Regulation of epithelium-specific Ets-like factors ESE-1 and ESE-3 in airway epithelial cells: potential roles in airway inflammation

Duan

Cao

et al. 2008

Cell Res

View full text Add to dashboard Cite

Airway inflammation is the hallmark of many respiratory disorders, such as asthma and cystic fibrosis. Changes in airway gene expression triggered by inflammation play a key role in the pathogenesis of these diseases. Genetic linkage studies suggest that ESE-2 and ESE-3, which encode epithelium-specific Ets-domain-containing transcription factors, are candidate asthma susceptibility genes. We report here that the expression of another member of the Ets family transcription factors ESE-1, as well as ESE-3, is upregulated by the inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in bronchial epithelial cell lines. Treatment of these cells with IL-1β and TNF-α resulted in a dramatic increase in mRNA expression for both ESE-1 and ESE-3. We demonstrate that the induced expression is mediated by activation of the transcription factor NF-κB. We have characterized the ESE-1 and ESE-3 promoters and have identified the NF-κB binding sequences that are required for the cytokine-induced expression. In addition, we also demonstrate that ESE-1 upregulates ESE-3 expression and downregulates its own induction by cytokines. Finally, we have shown that in Elf3 (homologous to human ESE-1) knockout mice, the expression of the inflammatory cytokine interleukin-6 (IL-6) is downregulated. Our findings suggest that ESE-1 and ESE-3 play an important role in airway inflammation.

show abstract

Effect of ozone and nitrogen dioxide on the permeability of bronchial epithelial cell cultures of non‐asthmatic and asthmatic subjects

Cited by 60 publications

References 31 publications

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

Non-animal models of epithelial barriers (skin, intestine and lung) in research, industrial applications and regulatory toxicology

The Role of Air Pollution in Adult-Onset Asthma: A Review of the Current Evidence

Regulation of epithelium-specific Ets-like factors ESE-1 and ESE-3 in airway epithelial cells: potential roles in airway inflammation

Contact Info

Product

Resources

About