Effect of oxygen tension on proliferation and characteristics of Wharton's jelly-derived mesenchymal stem cells

Widowati, Wahyu; Wijaya, Laura; Bachtiar, Indra; Gunanegara, Rimonta F.; Sugeng, Sri Utami; Irawan, Yudha Aryadi; Sumitro, Sutiman Bambang; Widodo, M. Aris

doi:10.1016/j.bgm.2014.02.001

Cited by 33 publications

(56 citation statements)

References 36 publications

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“…The cells were harvested and replated at a density 8×10 3 cells cmG 2 when cells reached 80-90% confluence. For normoxic studies, WJ-MSCs were cultured at 95% air (21% O 2 ) 5% CO 2 and for hypoxic cells were cultured at 5% O 2 (Nekanti et al, 2010;Widowati et al, 2014Widowati et al, , 2015.…”

Section: Materials and Methods Isolation And Culture Of Wj-mscsmentioning

confidence: 99%

“…The cells were analyzed by flow cytometry with a FACSCalibur TM 3 argon laser 488 nm (Becton Dickinson, Franklin Lakes, NJ, USA) using CellQuest Pro Acquisition on the BD FACStation TM software. The experiments and measurement of surface marker were performed in triplicate (Widowati et al, 2014(Widowati et al, , 2015.…”

Section: Immunophenotyping and Multipotent Differentiation Of Wj-mscsmentioning

confidence: 99%

“…A fast and efficient protocol for generation of large quantities of MSCs is required for clinical, therapeutic application and research requirements (Siegel et al, 2013;Lavrentieva et al, 2010;Nekanti et al, 2010). Oxygen concentration plays a role in maintaining the MSCs proliferation (Widowati et al, 2014). Hypoxic incubation can increases the MSCs proliferation without affecting surface markers and differentiation of WJ-MSCs at early and late passage compared to the normoxic tension (Nekanti et al, 2010;Widowati et al, 2014Widowati et al, , 2015.…”

Section: Introductionmentioning

confidence: 99%

“…Oxygen concentration plays a role in maintaining the MSCs proliferation (Widowati et al, 2014). Hypoxic incubation can increases the MSCs proliferation without affecting surface markers and differentiation of WJ-MSCs at early and late passage compared to the normoxic tension (Nekanti et al, 2010;Widowati et al, 2014Widowati et al, , 2015.…”

Section: Introductionmentioning

confidence: 99%

“…The previous research showed that Conditioned Medium from normoxic WJMSCs (WJMSCs-norCM) and hypoxic WJMSCs (WJMSCs-hypoCM) could inhibit proliferation of various cancer cell lines and caused low inhibition or have no cytotoxic effect on normal cells including mouse fibroblast cells, hMSCs, human fibroblast cells (Widowati et al, 2014). In this study, we performed the continuing research to measure the tumoricidal property of cell-free lysate of normoxia-treated WJMSCs (WJMSCs-norCL) and hypoxia-treated WJMSCs (WJMSCs-hypoCL) towards cancer cell lines including cervical (HeLa), liver (HepG2), ovarian (SKOV3) and oral squamosa (HSC3) cancer compared to human fibroblast cells, mouse fibroblast cells (NIH3T3) and human Mesenchymal Stem Cells (hMSCs).…”

Section: Introductionmentioning

confidence: 99%

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Tumoricidal Property of Normoxia and Hypoxia Cell-Free Lysate of Wharton’s Jelly-Mesenchymal Stem Cells Toward Various Cancer Cells

Widowati¹,

Wijaya²,

Agustina³

et al. 2015

International J. of Cancer Research

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Cancer is one of the leading causes of mortality and morbidity throughout the world. Since there are still some problems related to the conventional therapies for cancer treatment, it is critical to explore new more efficient therapy strategies. Mesenchymal Stem Cells (MSCs) are one of powerful tools for tissue engineering for regenerative medicine, as recent research aims to utilize MSCs for anti-cancer treatment. Our previous research demonstrated that Conditioned Medium from Whartons' Jelly MSCs (WJ-MSCs-CM) significantly lowered cancer proliferation of various cancer cell lines. This research was performed to evaluate the tumoricidal property of cell lysate from WJ-MSCs from normoxia (WJMSCs-norCL) and hypoxia-treated WJMSCs (WJMSCs-hypoCL) on the proliferation of human cancer cells, including cervical (HeLa), liver (HepG2), ovarian (SKOV3) and oral squamous (HSC3) cancer cell lines compared to normal cells including mouse fibroblast (NIH3T3), human Mesenchymal Stem Cells (hMSCs), human fibroblast. The WJMSCs-norCL and WJMSCs-hypoCL have cytotoxic activity, reduce proliferation of various cancer cell lines with minimum inhibitory concentration (IC 50 ) 21.094-95.928 μg mLG 1 and no cytotoxic to normal cells with IC 50 409, 191-629, 799.738 μg mLG 1 . The WJMSCs-norCL and WJMSCs-hypoCL inhibit proliferation in various cancer cell lines and are not toxic for normal cells.

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Section: Materials and Methods Isolation And Culture Of Wj-mscsmentioning

confidence: 99%

Section: Immunophenotyping and Multipotent Differentiation Of Wj-mscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Tumoricidal Property of Normoxia and Hypoxia Cell-Free Lysate of Wharton’s Jelly-Mesenchymal Stem Cells Toward Various Cancer Cells

Widowati¹,

Wijaya²,

Agustina³

et al. 2015

International J. of Cancer Research

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Oxygen Level Modifies the Expression of Genes Involved in the Epigenetic Regulation of Multipotent Stromal Cells In Vitro

2023

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Conditioned Medium – Is it an Undervalued Lab Waste with the Potential for Osteoarthritis Management?

Rosochowicz

Lach

Richter

et al. 2023

Stem Cell Rev and Rep

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Background The approaches currently used in osteoarthritis (OA) are mainly short-term solutions with unsatisfactory outcomes. Cell-based therapies are still controversial (in terms of the sources of cells and the results) and require strict culture protocol, quality control, and may have side-effects. A distinct population of stromal cells has an interesting secretome composition that is underrated and commonly ends up as biological waste. Their unique properties could be used to improve the existing techniques due to protective and anti-ageing properties. Scope of Review In this review, we seek to outline the advantages of the use of conditioned media (CM) and exosomes, which render them superior to other cell-based methods, and to summarise current information on the composition of CM and their effect on chondrocytes. Major Conclusions CM are obtainable from a variety of mesenchymal stromal cell (MSC) sources, such as adipose tissue, bone marrow and umbilical cord, which is significant to their composition. The components present in CMs include proteins, cytokines, growth factors, chemokines, lipids and ncRNA with a variety of functions. In most in vitro and in vivo studies CM from MSCs had a beneficial effect in enhance processes associated with chondrocyte OA pathomechanism. General Significance This review summarises the information available in the literature on the function of components most commonly detected in MSC-conditioned media, as well as the effect of CM on OA chondrocytes in in vitro culture. It also highlights the need to standardise protocols for obtaining CM, and to conduct clinical trials to transfer the effects obtained in vitro to human subjects. Graphical Abstract

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Effect of oxygen tension on proliferation and characteristics of Wharton's jelly-derived mesenchymal stem cells

Cited by 33 publications

References 36 publications

Tumoricidal Property of Normoxia and Hypoxia Cell-Free Lysate of Wharton’s Jelly-Mesenchymal Stem Cells Toward Various Cancer Cells

Tumoricidal Property of Normoxia and Hypoxia Cell-Free Lysate of Wharton’s Jelly-Mesenchymal Stem Cells Toward Various Cancer Cells

Oxygen Level Modifies the Expression of Genes Involved in the Epigenetic Regulation of Multipotent Stromal Cells In Vitro

Conditioned Medium – Is it an Undervalued Lab Waste with the Potential for Osteoarthritis Management?

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