Effect of oxygen on batch and continuous cultures of a nitrogen-fixing <i>Arthrobacter</i> sp.

Cacciari, I.; Lippi, D.; Bordeleau, L. M.

doi:10.1139/m79-108

Cited by 22 publications

(6 citation statements)

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“…We anticipated that we would find nifH genes from the isolates in our samples. These isolates were characterised as nitrogen fixers by previous studies (Cacciari et al, 1979;Lifshitz et al, 1986;Videira et al, 2009). …”

Section: Sequencing and Phylogenetic Analysismentioning

confidence: 97%

High diversity of nitrogen-fixing bacteria in the upper reaches of the Heihe River, northwestern China

et al. 2013

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Vegetation plays a key role in water conservation in the southern Qilian Mountains (northwestern China), located in the upper reaches of the Heihe River. Nitrogen-fixing bacteria are crucial for the protection of the nitrogen supply for vegetation in the region. In the present study, nifH gene clone libraries were established to determine differences between the nitrogen-fixing bacterial communities of the Potentilla parvifolia shrubland and the Carex alrofusca meadow in the southern Qilian Mountains. All of the identified nitrogen-fixing bacterial clones belonged to the Proteobacteria. At the genus level, Azospirillum was only detected in the shrubland soil, while Thiocapsa, Derxia, Ectothiorhodospira, Mesorhizobium, Klebsiella, Ensifer, Methylocella and Pseudomonas were only detected in the meadow soil. The phylogenetic tree was divided into five lineages: lineages I, II and III mainly contained nifH sequences obtained from the meadow soils, while lineage IV was mainly composed of nifH sequences obtained from the shrubland soils. The Shannon–Wiener index of the nifH genes ranged from 1.5 to 2.8 and was higher in the meadow soils than in the shrubland soils. Based on these analyses of diversity and phylogeny, the plant species were hypothesised to influence N cycling by enhancing the fitness of certain nitrogen-fixing taxa. The number of nifH gene copies and colony-forming units (CFUs) of the cultured nitrogen-fixing bacteria were lower in the meadow soils than in the shrubland soils, ranging from 0.4 × 10⁷ to 6.9 × 10⁷ copies g⁻¹ soil and 0.97 × 10⁶ to 12.78 × 10⁶ g⁻¹ soil, respectively. Redundancy analysis (RDA) revealed that the diversity and number of the nifH gene copies were primarily correlated with aboveground biomass in the shrubland soil. In the meadow soil, nifH gene diversity was most affected by altitude, while copy number was most impacted by soil-available K. These results suggest that the nitrogen-fixing bacterial communities beneath Potentilla were different from those beneath Carex

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Section: Sequencing and Phylogenetic Analysismentioning

confidence: 97%

High diversity of nitrogen-fixing bacteria in the upper reaches of the Heihe River, northwestern China

et al. 2013

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“…isolated from soil sampled in China (Ding et al 1981), and a fluorescent Arthrobacter sp. exhibits acetylene reduction activity that is correlated to oxygen tensions (Cacciari et al 1979). Even Proprionibacteria isolates have been reported to fix nitrogen (Baranova and Gogotov 1974).…”

Section: Non-frankia Nitrogen-fixing Actinobacteriamentioning

confidence: 99%

Phylogenetic perspectives of nitrogen-fixing actinobacteria

et al. 2011

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It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. During the last decade, Mycobacterium flavum, Corynebacterium autotrophicum and a fluorescent Arthrobacter sp. have been reported to have nitrogenase activity, but these studies have not been further verified. Additional reports of nitrogen fixation by Agromyces, Microbacterium, Corynebacterium and Micromonospora isolated from root nodules of leguminous and actinorhizal plants have increased. For several actinobacteria, nitrogen fixation was demonstrated by the ability to grow on nitrogen-free medium, acetylene reduction activity, 15N isotope dilution analysis and identification of a nifH gene via PCR amplification. Moreover, the analyses of draft genome sequences of actinobacteria including Slackia exigua, Rothia mucilaginosa and Gordonibacter pamelaeae have also revealed the presence of nifH-like sequences. Whether these nifH sequences are associated with effective nitrogen fixation in these actinobacteria taxa has not yet been demonstrated. These genes may be vertically or horizontally transferred and be silent sequences. These ideas merit further investigation. This minireview presents a phylogenetic comparison of nitrogen fixation gene (nifH) with the aim of elucidating the processes underlying the evolutionary history of this catalytic ability among actinobacteria.

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“…In addition, other members of Actinobacteria such as Arthrobacter sp., Agromyces sp., Corynebacterium autitrophicum, Mycobacterium, Micromonospora sp., Propionibacteria and Streptomyces sp. isolates have been reported to have nitrogen-fixing capacity (Cacciari et al, 1981;Berndt et al, 1989;Biabani et al, 2012;Gtari et al, 2012). Additionally, BLAST analysis identified three additional actinobacterial taxa possessing nifH homologs: Rothia mucilaginosa, Gordonibacter pamelaeae and Slackia exigua (Gtari et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Aspects of nitrogen-fixingActinobacteria, in particular free-living and symbioticFrankia

Sellstedt

Richau

2013

FEMS Microbiol Lett

173

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Studies of nitrogen-fixing properties among the Gram-positive Actinobacteria revealed that some species of Arthrobacter, Agromyces, Corynebacterium, Mycobacterium, Micromonospora, Propionibacteria and Streptomyces have nitrogen-fixing capacity. This is also valid for Frankia that fix nitrogen both in free-living and in symbiotic conditions. Frankia symbiosis results from interaction between the Frankia bacteria and dicotyledonous plants, that is, actinorhiza. These plants, which are important in forestry and agroforestry, form, together with the legumes (Fabales), a single nitrogen-fixing clade. It has been shown that a receptor-like kinase gene, SymRK, is necessary for nodulation in actinorhizal plants as well as in legumes and arbuscular mycorrhizal fungi. Recently, the involvement of isoflavonoids as signal molecules during nodulation of an actinorhizal plant was shown. The genome sizes of three Frankia species, Frankia EANpec, ACN14a and CcI3, are different, revealing a relationship between genome size and geographical distribution. Recent genomic sequencing data of Frankia represent genomes from cluster I to IV, indicating that the genome of DgI is one of the smallest genomes in Frankia. In addition, nonsymbiotic Frankiales such as Acidothermus cellulolyticus, Blastococcus saxoobsidens, Geodermatophilus obscurus and Modestobacter marinus have a variety of genome sizes ranging from 2.4 to 5.57 Mb.

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Effect of oxygen on batch and continuous cultures of a nitrogen-fixing Arthrobacter sp.

Cited by 22 publications

References 0 publications

High diversity of nitrogen-fixing bacteria in the upper reaches of the Heihe River, northwestern China

High diversity of nitrogen-fixing bacteria in the upper reaches of the Heihe River, northwestern China

Phylogenetic perspectives of nitrogen-fixing actinobacteria

Aspects of nitrogen-fixingActinobacteria, in particular free-living and symbioticFrankia

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