Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos

Thompson, Jeremy G.; Simpson, A. C.; Pugh, P.A.; Donnelly, P. E.; Tervit, H.R.

doi:10.1530/jrf.0.0890573

Cited by 286 publications

(148 citation statements)

References 17 publications

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“…Later studies on sheep and bovine animals [24][25][26] confirmed a positive impact of low oxygen levels on number of cells, morula and blastocyst formation. However, this improvement had no significant effect on pregnancy rates or on the viability of developing fetuses.…”

Section: Discussionmentioning

confidence: 95%

The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study

Kasterstein

Strassburger

Komarovsky

et al. 2013

J Assist Reprod Genet

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Purpose This prospective randomized study used sibling oocytes of 258 women with ≥8 oocytes to compare the effect of 5 % O 2 versus 20 % O 2 concentrations on embryo development and clinical outcome. Methods Oocytes of each case were divided between incubators with either 5 % or 20 % O 2 concentration. Outcome measures were fertilization, cleavage, embryo quality, blastocyst formation, and implantation, pregnancy and live birth rates. Results Fertilization and cleavage rates were similar in both groups. The 5 % O 2 group had significantly more blastomeres (P<0.05) and more top-quality embryos on day 3 (P<0.02), as well as significantly more available embryos for transfer (31.6 % vs. 23.1 % for the 20 % O 2 group; P<0.0001). There were significantly more cycles with good embryos in the 5 % group (76/258) than in the 20 % group (38/258) (P<0.0001). Implantation and pregnancy rates were significantly higher for 5 % O 2 embryos (P<0.03 and P<0.05, respectively). Live birth rates per embryo transfer were 34.2 % and 15.8 %, respectively, P<0.05. Conclusions Implantation, pregnancy and live birth rates are higher, and more good quality embryos are available for transfer and freezing with reduced rather than with atmospheric oxygen concentrations during embryo incubation.

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Section: Discussionmentioning

confidence: 95%

The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study

Kasterstein

Strassburger

Komarovsky

et al. 2013

J Assist Reprod Genet

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“…Oxygen tension has been known to influence embryo development and cell numbers in a number of species, and improvements in embryo development have been observed under lower oxygen tensions (5 % or 7 %) rather than under an atmospheric oxygen condition (20 %) [23][24][25][26]. Several reports suggested that mouse and bovine embryos have the capacity to detect and respond to low oxygen environment with changes in the expression of oxygen-regulated genes such as glucose transporter-1 (GLUT-1), GLUT-3, and vascular endothelial growth factor (VEGF) [7][8][9]27].…”

Section: Discussionmentioning

confidence: 99%

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

Yoon

Juhn

et al. 2012

J Assist Reprod Genet

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Purpose Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1α protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1α in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1α in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1α expression. Methods Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O 2 in both the absence and presence of IGF-I. Results The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1α protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O 2 exhibited significantly higher total cell numbers (83.4±18.1) and lower apoptotic index (3.7±1.5) than those cultured under 20 % O 2 (67.4±15.6) (6.9±3.5) (P<0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O 2 group. Conclusions HIF-1α may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-apoptotic effect, instead of increasing HIF-1α protein expression.

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“…However, the published results using 20% or 5% O 2 during the IVP steps are still controversial. Some research in bovine demonstrates that culture in lower oxygen tension (from 5 to 10%) improves maturation and embryo development (Nakao & Nakatsuji, 1990;Thompson et al, 1990;Voelkel & Hu, 1992) whilst others observed better results when using 20% O 2 (Oyamada & Fukui, 2004;Castro e Paula & Hansen, 2007). Moreover, evidences of interaction between culture medium and oxygen tension were also reported (Noda et al, 1994;Castro e Paula & Hansen, 2007).…”

Section: Introductionmentioning

confidence: 99%

The effect of interaction between macromolecule supplement and oxygen tension on bovine oocytes and embryos culturedin vitro

Mingoti

Castro

Méo

et al. 2009

Zygote

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SummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin -BSA, polyvinyl alcohol -PVA, polyvinyl pyrrolidone -PVP, Ficoll, KnockoutSR, or fetal calf serum -FCS) and oxygen tension [5% CO 2 in air (20% O 2 ) or 5% CO 2 , 5% O 2 and 90% N 2 (5% O 2 )] on oocyte maturation and embryo development. Nuclear progression to germinal vesicle breakdown, metaphase I and metaphase II stages were evaluated and overall results revealed that undefined (FCS) and semi-defined (BSA) media gave better results at 20% O 2 and defined media (PVA, PVP and Ficoll) at 5% O 2 . Independent of macromolecule supplement, IVM at 20% O 2 was considered optimal for nuclear maturation. To evaluate embryo development, oocytes matured in the previously described conditions were fertilized and cultured at the same oxygen tension used for IVM and assessed for cleavage (43.0 to 74.8%) and development to morulae (16.4 to 33.8%), blastocyst (7.7 to 52.9%) and hatched blastocyst (9.6 to 48.1%). Apart from oxygen tension, all treatments, except Knockout (22.7%), gave similar results for blastocyst development (26.5 to 38.7%). Independently of macromolecule supplement, higher development rates were obtained in an oxygen tension of 20% O 2 (67.4% cleavage, 29.2% morulae, 40.8% blastocyst and 34.0% hatched blastocyst) when compared with 5% O 2 (52.5, 21.8, 18.2 and 15.6%, respectively). This study indicates that BSA, PVA, PVP and Ficoll can replace serum during IVM and that the optimal atmospheric condition for in vitro production of bovine embryos is 5% CO 2 and 20% O 2 .

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Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos

Cited by 286 publications

References 17 publications

The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study

The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study

Effects of oxygen tension and IGF-I on HIF-1α protein expression in mouse blastocysts

The effect of interaction between macromolecule supplement and oxygen tension on bovine oocytes and embryos culturedin vitro

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