Effect of oxyethilated glycerol cryoprotectants on DPPC model lipid membranes structure and phase

Krasnikova, A. O.; Kasian, N. A.; Vashchenko, O. V.; Zinchenko, A. V.; Kompaniets, A. M.; Lisetski, L. N.; Soloviov, Dmytro; Булавин, Л. А.

doi:10.15407/cryo25.02.186

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2020

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“…Lipid membranes are an important component of living organisms, because they regulate plenty of the processes that are required to maintain the viability of every cell and cell organelles. That is why a considerable number of works on biophysics is devoted to the study of phase transitions in lipid membranes and their dependence on the temperature and pressure [1][2][3][4], the structural characteristics of various lipid phases [5,6], the influence of various impurities on the properties of membranes [7][8][9][10][11], as well as to the measurement of the dynamic characteristics of singleand multicomponent membranes [12,13].…”

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confidence: 99%

Influence of Cholesterol Concentration on Bacteriorhodopsin Photocycle

et al. 2020

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The photocycle of the membrane protein bacteriorhodopsin in Dipalmitoylphosphatidylcholine(DPPC)/Cholesterol membranes with various cholesterol concentrations has been studied using the time-resolved spectroscopy method. The temperature dependences of the rate constants of bacteriorhodopsin transitions between transient states are shown to satisfy the Eyring equation. It is proved that the growth of the cholesterol concentration in the DPPC membrane accelerates the bacteriorhodopsin photocycle.

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