Effect of oxide nanoparticles on the morphology and fluidity of phospholipid membranes and the role of hydrogen bonds

Wei, Xiaoran; Yu, Junchao; Ding, Lei; Hu, Jingtian; Jiang, Wei

doi:10.1016/j.jes.2017.02.011

Cited by 23 publications

(19 citation statements)

References 36 publications

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“…The same reasoning is generally invoked to explain why, in vitro, cationic NPs are more toxic than anionic NPs to Gram-negative bacteria 28 , 29 . Nevertheless, electrostatic attraction is not a necessary ingredient to the formation of stable NP-bilayer complexes, nor to toxicity, which can take place also when the NP and the membrane have a Z-potential of the same sign 24 , 28 , 30 .…”

Section: Introductionmentioning

confidence: 99%

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Non-disruptive uptake of anionic and cationic gold nanoparticles in neutral zwitterionic membranes

Canepa

Salassi

Simonelli

et al. 2021

Sci Rep

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The potential toxicity of ligand-protected nanoparticles (NPs) on biological targets is crucial for their clinical translation. A number of studies are aimed at investigating the molecular mechanisms shaping the interactions between synthetic NPs and neutral plasma membranes. The role played by the NP surface charge is still widely debated. We compare, via liposome leakage assays, the perturbation induced by the penetration of sub-6 nm anionic and cationic Au NPs into model neutral lipid membranes composed of the zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Our charged Au NPs are functionalized by a mixture of the apolar 1-octanethiol and a ω-charged thiol which is either the anionic 11-mercapto-1-undecanesulfonate or the cationic (11-mercaptoundecyl)-N,N,N-trimethylammonium. In both cases, the NP uptake in the bilayer is confirmed by quartz crystal microbalance investigations. Our leakage assays show that both negatively and positively charged Au NPs do not induce significant membrane damage on POPC liposomes when penetrating into the bilayer. By means of molecular dynamics simulations, we show that the energy barrier for membrane penetration is the same for both NPs. These results suggest that the sign of the NP surface charge, per se, does not imply different physicochemical mechanisms of interaction with zwitterionic lipid membranes.

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Section: Introductionmentioning

confidence: 99%

“…for example, for positively and negatively charged metal or oxide NPs interacting with charged bilayers [23][24][25] . The interaction between oppositely charged NPs and lipid bilayers can cause transient damage to the membrane, as well.…”

mentioning

confidence: 99%

Non-disruptive uptake of anionic and cationic gold nanoparticles in neutral zwitterionic membranes

Canepa

Salassi

Simonelli

et al. 2021

Sci Rep

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“…In membrane fusion events, lipid packing defects are considered as an initial step required for two adjacent membranes to fuse. 30,37,38 Since previous studies on NP-membrane interactions have shown that SiO2 NPs produce perturbations in lipid packing and membrane fluidity, 31,33,39 we used Laurdan spectral imaging to quantify changes in lipid packing and membrane hydration of DOPC GUVs labelled with 0.5 mol% Laurdan after incubation with 25 µg/ml SiO2 NPs.…”

Section: Influence Of Lipid Packing and Membrane Tension On Fusion Processesmentioning

confidence: 99%

Biomimetic Curvature and Tension-Driven Membrane Fusion Induced by Silica Nanoparticles

Perez¹,

Beales

2021

Preprint

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Membrane fusion is a key process to develop new technologies in synthetic biology, where artificial cells function as biomimetic chemical microreactors. Fusion events in living cells are intricate phenomena that require the coordinate action of multicomponent protein complexes. However, simpler synthetic tools to control membrane fusion in artificial cells are highly desirable. Native membrane fusion machinery mediates fusion driving a delicate balance of membrane curvature and tension between two closely apposed membranes. Here we show that silica nanoparticles (SiO2 NPs) at a size close to the cross-over between tension-driven and curvature-driven interaction regimes initiate efficient fusion of biomimetic model membranes. Fusion efficiency and mechanisms are studied by Förster Resonance Energy Transfer (FRET) and confocal fluorescence microscopy. SiO2 NPs induce a slight increase in lipid packing likely to increase the lateral tension of the membrane. We observe a connection between membrane tension and fusion efficiency. Finally, real-time confocal fluorescence microscopy reveals three distinct mechanistic pathways for membrane fusion. SiO2 NPs show significant potential for inclusion in the synthetic biology toolkit for membrane remodelling and fusion in artificial cells.

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“…78,79 Moreover, the combination of electrostatic attraction and hydrogen bonding between NPs and membranes was probably one of the reasons for membrane disruption and gelation. 80…”

Section: Membrane Damagementioning

confidence: 99%

Evaluation of immunoresponses and cytotoxicity from skin exposure to metallic nanoparticles

Wang

Lai

Shao

et al. 2018

IJN

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Nanotechnology is an interdisciplinary science that has developed rapidly in recent years. Metallic nanoparticles (NPs) are increasingly utilized in dermatology and cosmetology, because of their unique properties. However, skin exposure to NPs raises concerns regarding their transdermal toxicity. The tight junctions of epithelial cells form the skin barrier, which protects the host against external substances. Recent studies have found that NPs can pass through the skin barrier into deeper layers, indicating that skin exposure is a means for NPs to enter the body. The distribution and interaction of NPs with skin cells may cause toxic side effects. In this review, possible penetration pathways and related toxicity mechanisms are discussed. The limitations of current experimental methods on the penetration and toxic effects of metallic NPs are also described. This review contributes to a better understanding of the risks of topically applied metallic NPs and provides a foundation for future studies.

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Effect of oxide nanoparticles on the morphology and fluidity of phospholipid membranes and the role of hydrogen bonds

Cited by 23 publications

References 36 publications

Non-disruptive uptake of anionic and cationic gold nanoparticles in neutral zwitterionic membranes

Non-disruptive uptake of anionic and cationic gold nanoparticles in neutral zwitterionic membranes

Biomimetic Curvature and Tension-Driven Membrane Fusion Induced by Silica Nanoparticles

Evaluation of immunoresponses and cytotoxicity from skin exposure to metallic nanoparticles

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