Effect of Oxidative Stress-Induced Apoptosis on Active FGF23 Levels in MLO-Y4 Cells: The Protective Role of 17-β-Estradiol

Domazetovic, Vladana; Falsetti, Irene; Ciuffi, Simone; Iantomasi, Teresa; Marcucci, Gemma; Vincenzini, Massimo; Brandi, Maria Luisa

doi:10.3390/ijms23042103

Cited by 12 publications

(12 citation statements)

References 83 publications

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“…Numerous signaling pathways are involved in the osteoblast process, containing the Wnt/β-catenin, ERK1/2, and phosphatidylinositide-3 kinase (PI3K)/Akt pathways. 33 In previous studies, we had found that C3G promotes the proliferation of MC3T3-E1 without Wnt/β-catenin. 15 In this study, it was illustrated that the signaling pathways ERK1/2 had some relationships with C3G's capacity for inducing osteoblast differentiation.…”

Section: ■ Discussionmentioning

confidence: 93%

Cyanidin-3-glucoside Regulates Osteoblast Differentiation via the ERK1/2 Signaling Pathway

Chen

et al. 2021

ACS Omega

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Osteoporosis, characterized by a gradual decrease in the number of osteoblasts and a gradual increase in bone resorption of osteoclasts in bone tissue, is a global chronic disease, which severely impairs the quality of life of the elderly. Therefore, it is extremely urgent to study the prevention and treatment of osteoporosis. It has been reported that anthocyanins can regulate bone metabolism and prevent osteoporosis. Cyanidin-3-O-glucoside (C3G), the most common type of anthocyanin in nature, widely exists in a variety of vegetables and fruits. Although it has been shown that C3G has multiple effects on osteoclasts, its impact(s) and underlying mechanism(s) on osteoblasts are still not clear. Here, we evaluated the effect of C3G on cell proliferation and differentiation of osteoblasts (extracted from the hip joint of patients with osteoporosis) and MC3T3-E1 (a kind of osteoblast cell line from mice). We also test the ability of osteoblasts to mineralize after C3G treatment. To find the underlying mechanism of the above effects, we further evaluated the role of the ERK signaling pathway in C3G regulation of osteoblasts. The results showed that C3G treatment enhanced osteoblast proliferation rate, osteoblast mineralization points, the mRNA levels and protein expression levels of OC (osteocalcin), and the level of ERK phosphorylation, which could be blocked by pretreatment with ERK signaling pathway inhibitor. The above results not only indicate that the ERK pathway was involved in C3G regulation of osteoblast differentiation but also provide strong suggestive evidence that osteoblasts may be promising targets in preventive and therapeutic strategies for osteoporosis.

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Section: ■ Discussionmentioning

confidence: 93%

Cyanidin-3-glucoside Regulates Osteoblast Differentiation via the ERK1/2 Signaling Pathway

Chen

et al. 2021

ACS Omega

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“…Postmenopausal osteoporosis is also related to enhanced osteocyte senescence and apoptosis [ 90 , 91 , 92 , 93 ]. Indeed, the activation of MAPK signaling due to oxidative stress mediates the increase in apoptosis, RANKL/OPG ratio, sclerostin, and FGF23 levels, and the decrease in autophagy in osteocytic MLO-Y4 cells [ 28 , 94 , 95 ]. In these cells, the high levels of glucose increase ROS production and apoptosis through the downregulation of the AMP-activated protein kinase (AMPK)/FOXO3a signaling pathway [ 96 ].…”

Section: Oxidative Stress and Osteoporosis: Principal Involved Molecu...mentioning

confidence: 99%

“…All this can contribute to the activity of bone cells and thereby bone homeostasis by increasing and inhibiting osteoclastic and osteoblastic function [ 23 ]. Indeed, both oxidative stress and inflammation can be involved in the development of osteoporosis by preventing the differentiation of osteoblasts, inducing the differentiation and activity of osteoclasts, enhancing apoptotic osteocytes, and increasing the expression of RANKL and the RANKL/OPG-ratio [ 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress and Inflammation in Osteoporosis: Molecular Mechanisms Involved and the Relationship with microRNAs

Iantomasi

Romagnoli

Palmini

et al. 2023

IJMS

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Osteoporosis is characterized by the alteration of bone homeostasis due to an imbalance between osteoclastic bone resorption and osteoblastic bone formation. Estrogen deficiency causes bone loss and postmenopausal osteoporosis, the pathogenesis of which also involves oxidative stress, inflammatory processes, and the dysregulation of the expression of microRNAs (miRNAs) that control gene expression at post-transcriptional levels. Oxidative stress, due to an increase in reactive oxygen species (ROS), proinflammatory mediators and altered levels of miRNAs enhance osteoclastogenesis and reduce osteoblastogenesis through mechanisms involving the activation of MAPK and transcription factors. The present review summarizes the principal molecular mechanisms involved in the role of ROS and proinflammatory cytokines on osteoporosis. Moreover, it highlights the interplay among altered miRNA levels, oxidative stress, and an inflammatory state. In fact, ROS, by activating the transcriptional factors, can affect miRNA expression, and miRNAs can regulate ROS production and inflammatory processes. Therefore, the present review should help in identifying targets for the development of new therapeutic approaches to osteoporotic treatment and improve the quality of life of patients.

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“…Several potential mechanisms may explain the association between FlOPs and QUS parameters. Numerous lines of evidence suggest that oxidative stress is involved in the process of bone remodeling, inducing an imbalance between osteoclastic bone resorption and osteoblastic bone formation (7,38,39). Excessive ROS affects the differentiation and activity of osteoclasts by regulating mitogen-activated protein kinases (MAPKs), nuclear factor-kappa (NF-κB), and Ca 2+ -mediated signaling cascades (40).…”

Section: Discussionmentioning

confidence: 99%

Association between global biomarker of oxidative stress and quantitative ultrasound parameters in middle-aged and elderly adults: A cross-sectional study

Shen

Liu²,

Zhao³

et al. 2023

Front. Public Health

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IntroductionWith the population aging, osteoporosis has become a major public health concern. Elevated oxidative stress is a vital detrimental factor for bone health. Compared to common oxidative stress-related biomarkers, Fluorescent Oxidation Products (FlOPs) reflect the global levels of oxidation from proteins, lipids, and DNA. Nevertheless, whether plasma FlOP levels are related to bone health measured by Quantitative ultrasound (QUS) is unclear. Thus, the present study examined the association between FlOPs and QUS parameters in middle-aged and elderly adults.MethodsThis community-based cross-sectional study was conducted in Changchun, northeast China. Plasma FlOPs were determined by a fluorescent microplate reader at a wavelength of 320/420 nm (excitation/emission). QUS parameters [speed of sound (SOS) and broadband ultrasound attenuation (BUA)] of the calcaneus were assessed by an ultrasound bone densitometer. We used multivariable linear regression to examine the association between FlOPs and QUS parameters.ResultsA total of 491 subjects were included in this study. Their average age was 65.2 years (standard deviation [SD]: 9.7 years). FlOPs were inversely associated with SOS (β for an increase of logarithmic interquartile range = −10.64; P = 0.018). Higher FlOP levels were marginally associated with lower SOS in females (β for an increase of logarithmic interquartile range = −9.68, P = 0.066), but not in males (β for an increase of logarithmic interquartile range = −11.84, P = 0.131). No significant relationship between FlOPs and BUA was observed.ConclusionsPlasma FlOP levels were inversely associated with SOS, but not with BUA in middle-aged and elderly adults.

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Effect of Oxidative Stress-Induced Apoptosis on Active FGF23 Levels in MLO-Y4 Cells: The Protective Role of 17-β-Estradiol

Cited by 12 publications

References 83 publications

Cyanidin-3-glucoside Regulates Osteoblast Differentiation via the ERK1/2 Signaling Pathway

Cyanidin-3-glucoside Regulates Osteoblast Differentiation via the ERK1/2 Signaling Pathway

Oxidative Stress and Inflammation in Osteoporosis: Molecular Mechanisms Involved and the Relationship with microRNAs

Association between global biomarker of oxidative stress and quantitative ultrasound parameters in middle-aged and elderly adults: A cross-sectional study

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