Effect of oxidative stress during repeated ovulation on the structure and functions of the ovary, oocytes, and their mitochondria

Miyamoto, Katsuhiro; Sato, Eisuke F.; Kasahara, Emiko; Jikumaru, Mika; Hiramoto, Keiichi; Tabata, Hisae; Katsuragi, Miyuki; Odo, Satoshi; Utsumi, Kozo; Inoue, Masayasu

doi:10.1016/j.freeradbiomed.2010.05.025

Cited by 117 publications

(84 citation statements)

References 42 publications

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“…Sterile non-heparinized tubes were used to collect blood samples from non-pregnant mice during the diestrus three agents was repeated 10 times in 30 days (27). To induce ozone inhalation, mice were placed in a halfopen system in which an ozonator (manufactured by Beijing Kang Er Xing Technology Development Co. Ltd., Beijing, China) had been placed on a rack.…”

Section: Hormone Examinationmentioning

confidence: 99%

The protective effects of human umbilical cord mesenchymal stem cells on damaged ovarian function: A comparative study

Zhang

Xiong

et al. 2016

BST

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Section: Hormone Examinationmentioning

confidence: 99%

The protective effects of human umbilical cord mesenchymal stem cells on damaged ovarian function: A comparative study

Zhang

Xiong

et al. 2016

BST

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“…In other studies, L-carnitine supplementation was associated with decreased oocyte cytoskeletal damage (Mansour et al 2009) and reversed the negative effects of repeated superovulation, i.e. the decreased number and abnormal distribution of mitochondria and impaired development to blastocyst (Miyamoto et al 2010).…”

Section: Metabolism Of Fatty Acids For the Production Of Atpmentioning

confidence: 79%

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

2014

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Metabolism and ATP levels within the oocyte and adjacent cumulus cells are associated with quality of oocyte and optimal development of a healthy embryo. Lipid metabolism provides a potent source of energy and its importance during oocyte maturation is being increasingly recognised. The triglyceride and fatty acid composition of ovarian follicular fluid has been characterised for many species and is influenced by nutritional status (i.e. dietary fat, fasting, obesity and season) as well as lactation in cows. Lipid in oocytes is a primarily triglyceride of specific fatty acids which differ by species, stored in distinct droplet organelles that re-localise during oocyte maturation. The presence of lipids, particularly saturated vs unsaturated fatty acids, in in vitro maturation systems affects oocyte lipid content as well as developmental competence. Triglycerides are metabolised by lipases that have been localised to cumulus cells as well as oocytes. Fatty acids generated by lipolysis are further metabolised by b-oxidation in mitochondria for the production of ATP. b-oxidation is induced in cumulus-oocyte complexes (COCs) by the LH surge, and pharmacological inhibition of b-oxidation impairs oocyte maturation and embryo development. Promoting b-oxidation with L-carnitine improves embryo development in many species. Thus, fatty acid metabolism in the mammalian COC is regulated by maternal physiological and in vitro environmental conditions; and is important for oocyte developmental competence.

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“…8 OS plays a critical role in the process of ovulation by inducing apoptosis of luteal cells within the ovary which perturbs the area in and around the ova and granulosa cells that potentially decrease oocyte and embryo viability. 9 The imbalance of OS in the follicular fluid environment of the ovaries can cause detrimental issues, such as poor oocyte development, embryo development and the overall outcome of pregnancy. 9 Antioxidant enzymes such as catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPX) may inactivate ROS and help protect oocyte and embryo.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress and outcome of antioxidant supplementation in patients with polycystic ovarian syndrome (PCOS)

Panti

Shehu

Saidu

et al. 2018

Int J Reprod Contracept Obstet Gynecol

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Background: PCOS is a condition with significant decrease in antioxidant with an increased risk of oxidative stress. Antioxidant supplementation has been shown to improve insulin sensitivity in PCOS and may improve outcome of management of PCOS. Objectives of this study were to determine the oxidative stress level of PCOS patients, to assess the effect of antioxidant supplementation on the outcome of management of PCOS and to compare with a control group.Methods: The study was a single blind randomised control trial involving 200 patients with PCOS. The study was conducted at Usmanu Danfodiyo University Teaching Hospital Sokoto. They were randomly divided into intervention and control groups and base line serum levels of oxidative stress marker, antioxidant enzymes, vitamins and minerals were determined. Antioxidant supplementation and placebo were given to the intervention and control groups respectively. All the patients had ovulation induction with clomiphene citrate and were followed up for 6 months. Outcome measures were clinical pregnancy or menstrual regularisation. Level of significance was <0.05.Results: There was statistical significance in the serum levels of oxidative stress marker, antioxidant enzymes, vitamins and minerals between the two groups (post intervention). Glutathione peroxidase (p = 0.001), superoxide dismutase (p = 0.0001), catalase (p= 0.0369), melondialdehyde (p= 0.007), Vitamin A, Vitamin C, Vitamin E (p = 0.0001), zinc and copper (p = 0.0001). The clinical pregnancy outcomes were 22 (22%) versus 2 (2%); live births 18 (18%) versus 2 (2%) and menstrual regularisation 48 (48%) versus 46 (46%) in the intervention and control groups respectively.Conclusions: Antioxidant supplementation in this study significantly affected pregnancy rate in patients with PCOS. Larger studies are suggested to revisit the conclusion of the Cochrane review that antioxidants supplementation had no significant role in female infertility.

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Effect of oxidative stress during repeated ovulation on the structure and functions of the ovary, oocytes, and their mitochondria

Cited by 117 publications

References 42 publications

The protective effects of human umbilical cord mesenchymal stem cells on damaged ovarian function: A comparative study

The protective effects of human umbilical cord mesenchymal stem cells on damaged ovarian function: A comparative study

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

Oxidative stress and outcome of antioxidant supplementation in patients with polycystic ovarian syndrome (PCOS)

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