Effect of oxidative stress and 3‐hydroxytyrosol on DNA methylation levels of miR‐9 promoters

D’Adamo, Stefania; Cetrullo, Silvia; Borzı̀, Rosa Maria; Flamigni, Flavio

doi:10.1111/jcmm.14657

Cited by 9 publications

(8 citation statements)

References 18 publications

(39 reference statements)

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“…Oxidative stress promotes peroxiredoxin (PRX) hyperoxidation, which disrupts normal physiological signaling and contributes to OA [ 26 ]. During OA progression, various inflammatory mediators, such as IL-1 β and INOs, increase ROS accumulation and oxidative stress, which causes mitochondrial and nuclear DNA damage and epigenetic changes in gene expression that contribute to the progression of OA [ 23 , 27 ]. ROS have a distinct contribution to oxidative stress in synovial inflammation [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis

Bao

Yan

et al. 2020

Oxidative Medicine and Cellular Longevity

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Synovial inflammation is a major pathological feature of osteoarthritis (OA), which is a chronic degenerative joint disease. Fibroblast-like synoviocytes (FLS), localized in the synovial membrane, are specialized secretory cells. During OA synovitis, FLS produce chemokines and cytokines that stimulate chondrocytes to secrete inflammatory cytokines and activate matrix metalloproteinases (MMPs) in FLS. Recent studies have demonstrated that sirtuin 3 (SIRT3) performs as a key regulator in maintaining mitochondrial homeostasis in OA. This study aims at ascertaining whether SIRT3 is involved in OA synovitis. The overexpression (OE) and knockdown (KD) of SIRT3 are established by short hairpin RNA (shRNA) and recombinant plasmid in human FLS. The anti-inflammatory effect of SIRT3 underlying in oleanolic acid- (OLA-) prevented interleukin-1β- (IL-1β-) induced FLS dysfunction is then evaluated in vitro. Additionally, the molecular mechanisms of SIRT3 are assessed, and the interaction between SIRT3 and NF-κB is investigated. The data suggested that SIRT3 can be detected in human synovial tissues during OA, and OLA could elevate SIRT3 expression. OE-SIRT3 and OLA exhibited equal authenticity to repress inflammation and reverse oxidative stress changes in IL-1β-induced human FLS dysfunction. KD-SIRT3 was found to exacerbate inflammation and oxidative stress changes in human FLS. Furthermore, it was found that SIRT3 could directly bind with NF-κB, resulting in the suppression of NF-κB activation induced by IL-1β in human FLS, which then repressed synovial inflammation in OA. In general, the activation of SIRT3 by OLA inhibited synovial inflammation by suppressing the NF-κB signal pathway in FLS, and this suggested that SIRT3 is a potential target for OA synovitis therapy.

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Section: Discussionmentioning

confidence: 99%

Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis

Bao

Yan

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…HT has been shown to counteract H 2 O 2 -induced miR-9 expression, thereby restoring the protein translation of its cognate target, SIRT-1 [37]. In humans, three independent genomic loci of miR-9 map to chromosomes 1q22 (MIR9-1), 5q14.3 (MIR9-2), and 15q26.1 (MIR9-3), and HT prevents the promoter demethylation of these three genes occurring in oxidative stress conditions [89]. This recent paper ascribed to HT an interesting role as an epigenetic modulator able to influence gene expression without changing the genetic profile.…”

Section: Olive-derived Compoundsmentioning

confidence: 99%

Nutraceutical Activity in Osteoarthritis Biology: A Focus on the Nutrigenomic Role

D’Adamo

Cetrullo

Panichi

et al. 2020

Cells

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Osteoarthritis (OA) is a disease associated to age or conditions that precipitate aging of articular cartilage, a post-mitotic tissue that remains functional until the failure of major homeostatic mechanisms. OA severely impacts the national health system costs and patients’ quality of life because of pain and disability. It is a whole-joint disease sustained by inflammatory and oxidative signaling pathways and marked epigenetic changes responsible for catabolism of the cartilage extracellular matrix. OA usually progresses until its severity requires joint arthroplasty. To delay this progression and to improve symptoms, a wide range of naturally derived compounds have been proposed and are summarized in this review. Preclinical in vitro and in vivo studies have provided proof of principle that many of these nutraceuticals are able to exert pleiotropic and synergistic effects and effectively counteract OA pathogenesis by exerting both anti-inflammatory and antioxidant activities and by tuning major OA-related signaling pathways. The latter are the basis for the nutrigenomic role played by some of these compounds, given the marked changes in the transcriptome, miRNome, and methylome. Ongoing and future clinical trials will hopefully confirm the disease-modifying ability of these bioactive molecules in OA patients.

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“…However this is not a univocal result [213]. Finally, in animals and in humans, HTyr may interact with several microRNAs [218,276] that regulate numerous cellular function including DICER function that is relevant to the redox state [277,278].…”

Section: Effect Of Evoo Voo Oo Leaf Extracts and Mpcs Onmentioning

confidence: 99%

Is Extra Virgin Olive Oil an Ally for Women’s and Men’s Cardiovascular Health?

Franconi

Campesi

Romani

2020

Cardiovascular Therapeutics

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Noncommunicable diseases are long-lasting and slowly progressive and are the leading causes of death and disability. They include cardiovascular diseases (CVD) and diabetes mellitus (DM) that are rising worldwide, with CVD being the leading cause of death in developed countries. Thus, there is a need to find new preventive and therapeutic approaches. Polyphenols seem to have cardioprotective properties; among them, polyphenols and/or minor polar compounds of extra virgin olive oil (EVOO) are attracting special interest. In consideration of numerous sex differences present in CVD and DM, in this narrative review, we applied “gender glasses.” Globally, it emerges that olive oil and its derivatives exert some anti-inflammatory and antioxidant effects, modulate glucose metabolism, and ameliorate endothelial dysfunction. However, as in prescription drugs, also in this case there is an important gender bias because the majority of the preclinical studies are performed on male animals, and the sex of donors of cells is not often known; thus a sex/gender bias characterizes preclinical research. There are numerous clinical studies that seem to suggest the benefits of EVOO and its derivatives in CVD; however, these studies have numerous limitations, presenting also a considerable heterogeneity across the interventions. Among limitations, one of the most relevant in the era of personalized medicine, is the non-attention versus women that are few and, also when they are enrolled, sex analysis is lacking. Therefore, in our opinion, it is time to perform more long, extensive and lessheterogeneous trials enrolling both women and men.

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Effect of oxidative stress and 3‐hydroxytyrosol on DNA methylation levels of miR‐9 promoters

Cited by 9 publications

References 18 publications

Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis

Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis

Nutraceutical Activity in Osteoarthritis Biology: A Focus on the Nutrigenomic Role

Is Extra Virgin Olive Oil an Ally for Women’s and Men’s Cardiovascular Health?

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