Effect of oviductal fluid proteins on buffalo sperm characteristics during cryopreservation

Imam, Shahnawaz; Ansari, M.R.; Ahmed, Nizamuddin; Kumaresan, A.

doi:10.1016/j.theriogenology.2007.05.066

Cited by 12 publications

(14 citation statements)

References 26 publications

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“…Recent evidence indicating that post-thaw semen characteristics of Murrah buffalo are improved when oviductal secretions are included in the cryopreservation extender (Imam et al, 2008) indicates that the secretions impart a protective effect on the sperm membranes subjected to freeze-thaw. The bovine oviduct is believed to maintain the optimal balance between reactive oxygen species and antioxidants through the activity of glutathione peroxidase, Superoxide dismutase, and catalase (Lapointe and Bilodeau, 2003), and catalase from OF has been shown to bind to sperm (Lapointe et al, 1998).…”

Section: Proteins In Ofmentioning

confidence: 99%

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Evidence that oviduct secretions influence sperm function: A retrospective view for livestock1

Killian¹

2011

Journal of Animal Science

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The mammalian oviduct has long been recognized as an organ essential for successful reproduction. Bovine, ovine, porcine, and equine animal models have offered clear advantages for oviduct study related to gamete physiology, fertilization, and early embryonic development. Livestock species are amenable to surgical alteration of the reproductive tract, estrous cycle manipulation, gamete cryopreservation, and AI, as well as in vitro fertilization and embryo production. Although most reproductive technology developed for livestock was intended to benefit production animal agriculture, these techniques are a treasure trove of tools for researchers to better understand how the oviduct influences gamete function. Oviduct secretions obtained from in vitro tissue cultures or via indwelling oviduct catheters have been used for analyses to define the protein, lipid, carbohydrate, enzyme, and electrolyte compositions of the secretions during the estrous cycle or in response to hormone treatment. Oviduct secretions or components purified from them have also been used in in vitro assays to assess their ability to bind to sperm, influence sperm viability, motility, sperm capacitation, the acrosome reaction, sperm-egg binding, and egg penetration, as well as subsequent embryonic development. Compelling data have emerged which show that the composition of secretions differs during the estrous cycle and that their composition differs whether they originate from the ampullary or isthmic regions of the oviduct. These differences in composition are functionally relevant and associated with different responses by sperm. Evidence indicatess that oviduct-specific glycoproteins, glycosaminoglycans, carbohydrates, norepinepherine, catecholamines, heat-shock protein, and osteopontin are components of the oviductal milieu that have the capacity to modulate sperm function. Future research on the livestock oviduct will likely define the role that oviduct secretions have in modulating sperm function and how these modifications ultimately affect fertilization and embryo development.

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Section: Proteins In Ofmentioning

confidence: 99%

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Evidence that oviduct secretions influence sperm function: A retrospective view for livestock1

Killian¹

2011

Journal of Animal Science

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“…2007). This protective effect might be due to certain proteins/factors in the oviduct during oestrus (non‐luteal phase) that protect sperm from oxidative and proteolytic damage and maintain its membrane as well as cellular integrity (Imam et al. 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Functional studies indicate that oviduct specific glycoproteins secreted from oviductal epithelium have a direct effect on maintaining motility, viability, acrosomal integrity (Kumaresan et al. 2005, 2006a; Imam et al. 2008) and DNA integrity of sperm (Ellington et al.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Oviductal Proteins on Structural and Functional Characteristics of Cryopreserved Sperm in Murrah Buffaloes

Imam

Mr²,

Kumar³

et al. 2010

Reproduction in Domestic Animals

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The present study was undertaken to elucidate the effect of non-luteal oviductal proteins on sperm characteristics in Murrah buffaloes. Oviducts from healthy buffaloes were collected immediately after slaughter and the oestrous cycle phase was determined as either luteal or non-luteal based on ovarian morphology. Non-luteal oviducts (n = 80) were flushed from the isthmic end of the oviduct with PBS, fluid was centrifuged at 10,000 g at 4 degrees C for 20 min and then dialysed and clarified. The supernatant obtained was lyophilized to concentrate the protein and stored at -20 degrees C till use. Sixteen good quality ejaculates from four Murrah buffalo bulls were collected using an artificial vagina. After fresh semen analysis, each ejaculate was split into two parts and extended in Tris-citrate-egg yolk glycerol dilutor. Part I of the split ejaculate was treated with non-luteal oviductal proteins at the dose rate of 1 mg/ml of diluted semen, while part II remained as control. The extended semen was equilibrated for 4 h at 5 degrees C, filled in 0.5 ml French straws, exposed to LN(2) vapour, plunged into LN(2) and then stored at -196 degrees C. The equilibrated and frozen-thawed semen was evaluated for sperm motility, viability, acrosomal integrity, cervical mucus penetration test and hypo-osmotic sperm swelling test (HOST). In frozen-thawed semen, the percentage of sperm motility, viability and acrosomal integrity was significantly (p < 0.05) higher in the treatment group compared to the control group. The incorporation of non-luteal oviductal proteins in the extender increased the ability of sperm to penetrate cervical mucus both after equilibration and the freeze-thaw process. Similarly, the proportion of sperm with intact plasma membrane, as revealed by HOST values, was also significantly (p < 0.05) higher in the treatment group (32.6%) than the control group (27%) in frozen-thawed semen. It was inferred that incorporation of non-luteal whole oviductal fluid proteins improved the sperm quality in frozen-thawed semen in Murrah buffaloes.

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“…The traditional HOS test, originally presented by Jeyendran et al (1984), enables reproduction specialists to determine the functional intactness of sperm membranes as spermatozoa "swell" under hypo-osmotic conditions due to the influx of water, and the expansion of the membranes causes the tails to coil. Several authors (Rodríguez-Martínez, 1998;Neild et al, 1999;Imam et al, 2008) have emphasized the suitability of the HOS test for assessing the quality of fresh and frozen-thawed semen in different farm animal species. A positive correlation was found between the results of the HOS test or its modifications and the non-return rate of female animals (Revell and Mrode, 1994;Correa et al, 1997a), which potentially makes it one of the most appropriate and simple methods for semen quality evaluation for the AI industry.…”

mentioning

confidence: 99%

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Padrik¹,

Hallap²,

Kaart³

et al. 2012

Czech J. Anim. Sci.

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ABSTRACT:As an attempt to find an inexpensive and simple laboratory method for artificial insemination (AI) bull semen quality assessment, the osmotic resistance of spermatozoa was measured using the hypoosmotic swelling (HOS) test, developed by Jeyendran et al. (1984) (labelled HOS-1), and its modifications (HOS-2, HOS-3), with decreased osmotic pressure aimed at challenging sperm survival ability. The test results were benchmarked against sperm viability measurements performed using the Computerized Motility Analyzer (CMA), and field fertility was calculated as non-return rate (NRR). Two age groups of Estonian Holstein bull sires were included in this study to test possible age effect on semen quality parameters. The HOS-1 test in fresh bull semen correlated well with sperm general motility (GMot) (r = 0.63, P < 0.001 at batch level and r = 0.77, P < 0.001 at bull level) as well as with progressive motility (PMot) in frozen-thawed (FT) semen (r = 66, P < 0.001 at batch level and r = 0.81, P < 0.001 at bull level), which makes the test suitable for the prediction of post-thaw semen quality. However, the HOS-2 and HOS-3 values in FT semen had high correlations with NRR (r = 0.65, r = 0.66, P < 0.001 at batch level and r = 0.63, r = 0.71, P < 0.01 at bull level), which was comparable to those between GMot and NRR or PMot and NRR. A combination of motility parameters and the results of the HOS-1 and HOS-3 tests provided a good model for predicting the potential fertility of bull semen. Values of sperm membrane post-thaw intactness, assessed using HOS-2, as well as of sperm motility measurements were higher in mature bulls compared to those in young bulls. Short conclusion: different modifications of the hypo-osmotic swelling test are useful for routine bovine semen quality assessment at AI stations.Keywords: bull fertility; semen quality; sperm membrane intactness; bull age Laboratory analyses have been used over many decades to evaluate semen quality. Despite the fact that none of the single assays developed provide results that consistently and highly correlate with fertility, the laboratory evaluation of semen samples remains an important procedure for the artificial insemination (AI) industry to eliminate low fertility bulls or semen from being used in artificial insemination programmes (Graham and Mocé, 2005).Post-thaw viability and fertility of cryopreserved sperm are often reduced due to accumulated cellular damage during the cryopreservation phases (Muiño et al., 2008). Decrease in temperature, cold shock, and intracellular ice formation can affect sperm plasma membrane, acrosomal and mitochondrial membrane integrity (Thomas et al., 1998;Defoin et al., 2008), and cause the loss of intracellular components (Graham and Mocé, 2005), which can initiate cell death.Sperm membrane integrity can be evaluated by several methods, e.g. light or fluorescent microscopy combined with vital stains (Brito et al., 2003), and flow cytometry (Januskauskas et al.,

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Effect of oviductal fluid proteins on buffalo sperm characteristics during cryopreservation

Cited by 12 publications

References 26 publications

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Evidence that oviduct secretions influence sperm function: A retrospective view for livestock1

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Evidence that oviduct secretions influence sperm function: A retrospective view for livestock1

Effect of Oviductal Proteins on Structural and Functional Characteristics of Cryopreserved Sperm in Murrah Buffaloes

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

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