Effect of ovariectomy on maxilla and mandible dimensions of female rats

Omori, Marjorie Ayumi; Marañón‐Vásquez, Guido Artemio; Romualdo, Priscilla Coutinho; Neto, Evandro Carneiro Martins; Stuani, Maria Bernadete Sasso; Matsumoto, Mírian Aiko Nakane; Nelson‐Filho, Paulo; Proff, Peter; León, Jorge Esquiche; Kirschneck, Christian; Küchler, Érika Calvano

doi:10.1111/ocr.12376

Cited by 12 publications

(8 citation statements)

References 57 publications

(87 reference statements)

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“…The success of the surgical procedure was confirmed by the animals' survival, gradual increase in body weight during the experimentation period and by the uterine atrophy after euthanasia in the experimental group (Omori et al, 2020). The decrease in endogenous estrogen release, caused by ovariectomy, provides significant differences in the body weight and uterine weight (Chen et al, 2014).…”

Section: Hypoestrogenism -Estrogen Deficiency Modelmentioning

confidence: 90%

“…To create estrogen deficiency (decrease the endogenous production of estrogen), a bilateral surgical excision of the ovaries (ovariectomy) was performed in the hypoestrogenism group. While the control group was submitted to fictitious surgery, in which the ovaries were moved and returned to their initial position, as previously described in Omori et al (2020).…”

Section: Hypoestrogenism -Estrogen Deficiency Modelmentioning

confidence: 99%

“…GAPDH (Rn01462661-g1) and ACTB (Rn01412977-g1) were used as endogenous controls. The relative levels of mRNA expression were determined by the 2 -∆∆ Cycle Threshold (2 -∆∆CT ) method (Omori et al, 2020). Both, GAPDH and ACTB, genes were used for sample normalization to calculate the relative quantification.…”

Section: Analysis Of Mmp1 Mmp8 E Mmp13 In Odontogenic Region -Rt-qpcrmentioning

confidence: 99%

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The impact of hypoestrogenism and occlusal function on MMP1, MMP8 and MMP13 expression in the odontogenic region in rats

Madalena

Judachesci

Küchler

et al. 2021

RSD

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Background: The impact of estrogen deficiency and occlusion in the matrix metalloproteinases (MMPs) expression in dental tissues has not yet been elucidated. Objective: To evaluate the influence of estrogen deficiency and occlusal hypofunction and hyperfunction on the gene expression of MMP1, MMP8 and MMP13 in the odontogenic region of teeth in continuous growth, in the murine model. Material and methods: Rats (Wistar Hannover lineage) were divided into two groups according to the intervention received: Hypoestrogenism Group - ovariectomy surgery and Control Group - fictitious surgery. Occlusal hypofunction and hyperfunction conditions were also established in all animals (each animal presented both conditions). After euthanasia, the hemimandibles were removed to evaluate the gene expression through real time PCR. T-test was used to compare the mean differences between groups (P<0.05). Results: There was no statistically significant difference of the relative gene expression of MMP1, MMP8 and MMP13 between the hypoestrogenism and control groups (P>0.05). A statistically significant difference of the relative MMP13 expression between the occlusal hypofunction and hyperfunction tooth was observed (P=0.03). In the hypoestrogenism group, MMP13 was overexpressed in hypofunction tooth (P=0.045). Conclusion: Occlusal function affects MMP13 expression in the odontogenic region, in murine model.

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Section: Hypoestrogenism -Estrogen Deficiency Modelmentioning

confidence: 90%

Section: Hypoestrogenism -Estrogen Deficiency Modelmentioning

confidence: 99%

Section: Analysis Of Mmp1 Mmp8 E Mmp13 In Odontogenic Region -Rt-qpcrmentioning

confidence: 99%

See 1 more Smart Citation

The impact of hypoestrogenism and occlusal function on MMP1, MMP8 and MMP13 expression in the odontogenic region in rats

Madalena

Judachesci

Küchler

et al. 2021

RSD

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“…According to a reported study on Japanese quails, E2-induced osteoclastogenesis can be regulated in the bone marrow cells via the RANK/RANKL/OPG (RANK/RANK Ligand/Osteoprotegerin) system during medullary bone formation ( Hiyama et al, 2019 ). Moreover, in prepubertal ovariectomy mice, E2 deficiency can promote both maxilla and mandible growth, accompanied by an increase in RANKL/OPG ratio ( Omori et al, 2020 ; Küchler et al, 2021 ). A low bone turnover in 20-day-old rats treated with E2 for 14 d was due to decreased bone remodeling ( Zhang et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis reveals the molecular mechanisms underlying osteoclast differentiation in the estrogen-deficient pullets

Yue¹,

Huang²,

Song³

et al. 2023

Poultry Science

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“…Noteworthy, previous studies reported the impact of estrogen on oral and dental tissues. It was reported that estrogen deficiency altered the gene expression involved in craniofacial growth sites and odontogenic area leading to alterations in the jaws bone length, condylar growth and morphological deterioration of tooth structure, respectively [9][10][11]. These effects are mediated by estrogen receptors (ERs) that are one of the ligand-activated transcription factors which control the cellular differentiation and growth.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical expression of estrogen receptor alpha in the maxillary sinus, pulp, and periodontal ligament of adjacent teeth in late pregnancy in rats

2022

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This study aimed to assess the histological changes in the maxillary sinus and its adjacent dental tissues as pulp and periodontal ligament during pregnancy and investigate the role of estrogen hormone in these changes through the detection of estrogen receptors in these tissues. Sixteen adult female rats were used and were allocated into two groups: control non-pregnant (n = 8) and pregnant (n = 8). They were sacrificed and their heads were prepared for histological and immunohistochemical examination for estrogen receptor alpha. Our results revealed that pregnant rats revealed inflammatory changes in the sinus as thick epithelial lining, loss of cilia, swollen goblet cells, intraepithelial and interstitial edema. The lamina propria demonstrated considerable infiltration of inflammatory cells, glandular hyperplasia with vacuolar degeneration, and vascular congestion. Periodontal ligament and pulp revealed hyperemia and vascular congestion. Immunohistochemical examination of estrogen receptor alpha in the maxillary sinus and adjacent dental tissues (Periodontal ligament and pulp) in pregnant rats revealed a significant increase in its expression in all examined tissues. In conclusion, there was an increase in expression of ERα in the sinus mucosa and dental tissues during pregnancy together with slight inflammatory changes in these tissues. Hence, dentists should be aware of the effect of these changes on the pregnant women avoiding teeth extraction due to misdiagnosis of dental, periodontal or sinus pain after exclusion of true pathologies.

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Effect of ovariectomy on maxilla and mandible dimensions of female rats

Abstract: Objective: The role of oestrogen in craniofacial growth still remains unclear. Therefore, the present study aimed to assess the effect of oestrogen deficiency on maxilla and mandible dimensions.

Cited by 12 publications

References 57 publications

The impact of hypoestrogenism and occlusal function on MMP1, MMP8 and MMP13 expression in the odontogenic region in rats

The impact of hypoestrogenism and occlusal function on MMP1, MMP8 and MMP13 expression in the odontogenic region in rats

Transcriptomic analysis reveals the molecular mechanisms underlying osteoclast differentiation in the estrogen-deficient pullets

Immunohistochemical expression of estrogen receptor alpha in the maxillary sinus, pulp, and periodontal ligament of adjacent teeth in late pregnancy in rats

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