Effect of osteopontin (OPN) on in vitro embryo development in cattle

Monaco, Elisa Lo; Gasparrini, B.; Boccia, Lorenzo; Rosa, Anna De; Attanasio, Laura B.; Zicarelli, L.; Killian, Gary J.

doi:10.1016/j.theriogenology.2008.08.012

Cited by 53 publications

(53 citation statements)

References 52 publications

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“…Supporting the mentioned results, the presence of osteopontin improved the efficiency of in vitro embryo production in bovine species (Monaco et al 2009). In addition, the exposure to osteopontin during IVF has also been shown to improve in vitro development of porcine embryos (Hao et al 2006(Hao et al , 2008.…”

Section: Osteopontinsupporting

confidence: 63%

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Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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Experimental evidence from the last 30 years supports the fact that the oviduct is involved in the modulation of the reproductive process in eutherian mammals. Oviductal secretion contains molecules that contribute to regulation of gamete function, gamete interaction, and the early stages of embryo development. The oviductal environment would act as a sperm reservoir, maintaining sperm viability, and modulating the subpopulation of spermatozoa that initiates the capacitation process. It could also contribute to prevent the premature acrosome reaction and to reduce polyspermy. Many studies have reported the beneficial effects of the oviductal environment on fertilization and on the first stages of embryo development. Some oviductal factors have been identified in different mammalian species. The effects of oviductal secretion on the reproductive process could be thought to result from the dynamic combined action (inhibitory or stimulatory) of multiple factors present in the oviductal lumen at different stages of the ovulatory cycle and in the presence of gametes or embryos. It could be hypothesized that the absence of a given molecule would not affect fertility as its action could be compensated by another factor with similar functions. However, any alteration in this balance could affect certain events of the reproductive process and could perhaps impair fertility. Thus, the complexity of the reproductive process warrants a continuous research effort to unveil the mechanisms and factors behind its regulation in the oviductal microenvironment.

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Section: Osteopontinsupporting

confidence: 63%

“…A study by Souza et al (2008) revealed that distribution of sperm-binding sites of osteopontin changed after incubation in the bovine oviductal fluid, and the authors suggested that the protein could participate in sperm-oocyte interaction. Another study has reported that osteopontin increased bovine sperm capacitation (Monaco et al 2009). …”

Section: Osteopontinmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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“…Moreover, some oviductal proteins have been shown to interact with gametes and to improve efficiency of IVF in porcine (McCauley et al 2003, bovine (Martus et al 1998), human (O' Day-Bowman et al 1996), and equine (Mugnier et al 2009) species. Some of these proteins, such as osteopontin, have been identified (Hao et al 2006, Goncalves et al 2008, Monaco et al 2009) in cattle and pigs and atrial natriuretic peptide A (Anderson et al 1994, Zamir et al 1995, Zhang et al 2006 and oviductin (oviduct-specific glycoprotein; Martus et al 1998, Buhi 2002, McCauley et al 2003 in cattle, pigs, and humans.…”

Section: Introductionmentioning

confidence: 99%

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Ambruosi¹,

Accogli²,

Douet³

et al. 2013

Reproduction

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Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus-oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization.

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“…Information about OPN roles in male reproduction has been demonstrated by experiments using in vitro fertilization (IVF). In this regard, incubation of bovine oocytes with OPN or fertilization of oocytes with sperm previously treated with OPN enhanced fertilization, cleavage rates and blastocyst development at days 8 and 11 (Goncalves et al, 2008;Monaco et al, 2009). Following this same conception, studies conducted with the swine species confirmed that OPN, when added to IVF media, also increased fertilization and embryo survival (Hao et al, 2006).…”

Section: Proteome Of Seminal Fluidmentioning

confidence: 85%