Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro

Yu, Congtao; Dai, Lifen; Ma, Zhaoxia; Zhao, Hongbin; Yuan, Yong; Zhang, Yunfeng; Bao, Pengfei; Su, Yi; Ma, Daiping; Liu, Chang; Wu, Xingfei; Liu, Jinxue; Li, Yanjiao; Wang, Bing; Hu, Min

doi:10.1186/s12906-019-2435-6

Cited by 11 publications

(9 citation statements)

References 19 publications

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“…To explore the cytoprotective effects of P. decorata , bone marrow-derived mesenchymal stem cells (bmMSCs) were isolated from bone marrow of femur and tibia of rats [16, 17]. bmMSCs are known to possess characteristics of stem cells, including self-renewal and multi-directional differentiation properties [18], and bmMSC transplantation has been applied to treat heart diseases [19], spinal cord injuries [20] and diabetes [21].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Cytoprotective effects of Pyrola decorata H. Andres and its five phenolic components

Chen

Liu

et al. 2019

BMC Complement Altern Med

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BackgroundPyrola decorata H. Andres, is exclusively distributed in China and a source of traditional Chinese herbal medicine Luxiancao for more than 2000 years. Here, we evaluated the antioxidant and cytoprotective effects of P. decorata and its five phenolic components (protocatechuic acid, gallic acid, hyperoside, 2′′-O-galloylhyperin, and quercetin), and discussed their antioxidant chemistry.MethodsA lyophilized aqueous extract of P. decorata (LAEP) was prepared and analyzed with high-performance liquid chromatography (HPLC). LAEP and its five phenolic components were comparatively investigated using five antioxidant assays, including ferric-reducing antioxidant power, cupric ion-reducing antioxidant capacity, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical (PTIO•)-scavenging, 1,1-diphenyl-2-picryl-hydrazl radical (DPPH•)-scavenging, and 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) radical (ABTS+•)-scavenging activities. The reaction products of the five phenolic components with 4-methoxy-2,2,6,6-tetramethylpiperidine-1-oxyl radical (4-methoxy-TEMPO•) were determined with ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) analysis. LAEP and its five phenolic components were incubated with bone marrow-derived mesenchymal stem cells (bmMSCs) subjected to oxidative stress to demonstrate their cytoprotective effects with a flow cytometry assay.ResultsIn the five antioxidant assays, LAEP and its five phenolic components dose-dependently increased the radical-scavenging (or reducing power) activities. However, the IC50 values of hyperoside were consistently higher than those of 2′′-O-galloylhyperin. UPLC-ESI-Q-TOF-MS/MS analysis results indicated that the five phenolics could yield dimer products in the presence of 4-methoxy-TEMPO• via the radical adduct formation (RAF) pathway. Flow cytometry assay results confirmed the cytoprotective activity of LAEP and its five phenolic components toward stressed bmMSCs. In particular, 2′′-O-galloylhyperin could more effectively reduce the percentage of damaged bmMSCs than hyperoside.ConclusionLAEP and its five phenolic components may undergo redox-based pathways (such as electron transfer and H+ transfer) and covalent-based pathway (i.e., RAF) to exhibit antioxidant activity. One consequence of RAF is the generation of phenolic-phenolic dimer. In both organic and aqueous media, 2′′-O-galloylhyperin exhibited higher redox-based antioxidant levels (or cytoprotective levels) than those with hyperoside. The differences could be attributed to 2′′-O-galloylation reaction.

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Section: Introductionmentioning

confidence: 99%

Antioxidant and Cytoprotective effects of Pyrola decorata H. Andres and its five phenolic components

Chen

Liu

et al. 2019

BMC Complement Altern Med

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“…Nutritional supplementation may also support bone healing. However, because the bone repair mechanism is complex, the non-invasive treatment methods and supplements that are widely used in recent times have not shown satisfactory effects [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Supplementation with osteokine, a natural compound used in traditional Chinese medicine, has been found to be effective in improving bone metabolism [ 5 , 6 ]. Osteokine is extracted from herbs that have been used to treat bone diseases in China for millennia [ 6 ]. Its formula is as follows: Chenpi (Citri Reticulatae Pericarpium), Hong Hua ( Carthami flos ), Sanqi ( Panax notoginseng ), Du Zhong ( Eucommia ulmoides ), Ren Shen (Panax ginseng), Huangqi ( Radix Astragali Mongolici ), and Bie Jia ( Carapax Trionycis ).…”

Section: Introductionmentioning

confidence: 99%

“…It regulates renal and splenic activity to nourish the bone and produce multiple effects on bone repair and the expression of various genes related to bone formation and angiogenesis [ 5 , 9 ]. Thus, osteokine is clinically used to treat various orthopedic conditions, including femoral head necrosis, lumbar intervertebral disc prolapse, fracture, and osteoarthritis [ 6 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Collagen- and Arginine-Fortified Osteokine Supplementation on Fracture Healing

Karaytuğ¹,

Arzu²,

Ergin³

et al. 2021

Cureus

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IntroductionDelayed union or nonunion is an important clinical challenge for orthopedic surgeons. In addition to the main treatment algorithms, the use of nutritional supplements is increasingly common. In this study, we investigated the effects of nutritional supplements fortified with arginine and collagen on fracture healing. Materials and methodsTwenty-four rats with femur fractures were divided into experimental and control groups. Intramedullary fixation was performed in both groups. 20 ml/kg nutritional supplement was given to the experimental group. Radiological examination was performed at third and sixth weeks, and histopathological examination was performed at the sixth week. ResultsNo statistically significant difference was found between the radiological scores of the groups at the third and sixth weeks. Nutritional supplement affected the histological properties of callus. Histological evidence of bone healing was observed by the sixth week in both groups but the score was higher in nutritional supplement group. A statistically significant difference was found between the histopathological scores of the groups at the sixth week. ConclusionArginine-and type two collagen-augmented traditional nutritional supplements may help to achieve more successful results in fracture healing.

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Osteoking prevents bone loss and enhances osteoblastic bone formation by modulating the AGEs/IGF‐1/β‐catenin/OPG pathway in type 2 diabetic db/db mice

Yang,

Li,

Wang

et al. 2024

Cell Biology International

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Type 2 diabetic osteoporosis (T2DOP) is a skeletal metabolic syndrome characterized by impaired bone remodeling due to type 2 diabetes mellitus, and there are drawbacks in the present treatment. Osteoking (OK) is widely used for treating fractures and femoral head necrosis. However, OK is seldom reported in the field of T2DOP, and its role and mechanism of action need to be elucidated. Consequently, this study investigated whether OK improves bone remodeling and the mechanisms of diabetes‐induced injury. We used db/db mice as a T2DOP model and stimulated MC3T3‐E1 cells (osteoblast cell line) with high glucose (HG, 50 mM) and advanced glycation end products (AGEs, 100 µg/mL), respectively. The effect of OK on T2DOP was assessed using a combined 3‐point mechanical bending test, hematoxylin and eosin staining, and enzyme‐linked immunosorbent assay. The effect of OK on enhancing MC3T3‐E1 cell differentiation and mineralization under HG and AGEs conditions was assessed by an alkaline phosphatase activity assay and alizarin red S staining. The AGEs/insulin‐like growth factor‐1(IGF‐1)/β‐catenin/osteoprotegerin (OPG) pathway‐associated protein levels were assayed by western blot analysis and immunohistochemical staining. We found that OK reduced hyperglycemia, attenuated bone damage, repaired bone remodeling, increased tibial and femoral IGF‐1, β‐catenin, and OPG expression, and decreased receptor activator of nuclear kappa B ligand and receptor activator of nuclear kappa B expression in db/db mice. Moreover, OK promoted the differentiation and mineralization of MC3T3‐E1 cells under HG and AGEs conditions, respectively, and regulated the levels of AGEs/IGF‐1/β‐catenin/OPG pathway‐associated proteins. In conclusion, our results suggest that OK may lower blood glucose, alleviate bone damage, and attenuate T2DOP, in part through activation of the AGEs/IGF‐1/β‐catenin/OPG pathway.

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Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro

Cited by 11 publications

References 19 publications

Antioxidant and Cytoprotective effects of Pyrola decorata H. Andres and its five phenolic components

Antioxidant and Cytoprotective effects of Pyrola decorata H. Andres and its five phenolic components

Effects of Collagen- and Arginine-Fortified Osteokine Supplementation on Fracture Healing

Osteoking prevents bone loss and enhances osteoblastic bone formation by modulating the AGEs/IGF‐1/β‐catenin/OPG pathway in type 2 diabetic db/db mice

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