Effect of osteoblast supernatants on cancer cell migration and invasion

Giunciuglio, Daniela; Cai, Tania; Filanti, C.; Manduca, Paola; Albini, Adriana

doi:10.1016/0304-3835(95)03955-v

Cited by 20 publications

(17 citation statements)

References 7 publications

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“…The production of C3 in tissues with high levels of collagen biosynthesis, as in bone, could attract preferentially circulating breast carcinoma cells and eventually other tumor cells that are attracted by C3, such as melanoma 2 and possibly prostatic carcinoma cells. The endogenous production of MMPs in tumor cells would be sustained by the presence of C3 in the homing site and this might favor their growth and the neoangiogenesis, in a fashion similar as that hypothesized above for primary tumors and with the end effect of promoting the colonization by C3-responsive cancer cells of C3-rich homing sites.…”

Section: Resultsmentioning

confidence: 99%

“…Increased expression of pro-MMP-2 and -9 is associated to the metastatic behavior of most tumors, including breast carcinoma, and in vitro the production of active forms of MMPs is associated with the induction of migratory behavior in cancer cells (18 -22). The migration of mouse melanoma, human breast, and prostatic carcinoma cell lines induced by CM from mature rat and rabbit osteoblasts is accompanied by induction of MMPs (2,3). Tissue inhibitors of metalloproteinases (TIMP) -1 and -2 play a regulatory role on proteolysis by MMPs (16 -19, 23).…”

mentioning

confidence: 99%

“…Mature osteoblasts conditioned medium (CM), 1 collected when sustained biosynthesis of collagen type I occurs during osteogenesis in vitro from differentiating cultures of rat, rabbit, human osteoblasts, and conditionally transformed human osteoblast-like line HFO, induces directional migration of endothelial and melanoma and breast and prostatic carcinoma cells but not of normal cells or of transformed mesenchymal cells in the classical in vitro double chamber Boyden assay (1)(2)(3)(4).…”

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confidence: 99%

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Pro-collagen I COOH-terminal Trimer Induces Directional Migration and Metalloproteinases in Breast Cancer Cells

Palmieri¹,

Poggi²,

Ulivi³

et al. 2003

Journal of Biological Chemistry

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Breast and prostatic carcinomas, melanoma, and endothelial cell lines are chemoattracted by medium conditioned by mature osteoblasts. The chemoattractant for endothelial cells was identified with C3, carboxyl-terminal trimer of pro-collagen type I. We report that C3 induces directional migration and proliferation, the expression of tissue inhibitor of metalloproteinases-2, prometalloproteinase-2 and -9, and their activation in MDA MB231 cells, without changing the expression of tissue inhibitor of metalloproteinases-1 and of metalloproteinase-14. Antiserum against metalloproteinase-2 or -9 or -14, tissue inhibitor of metalloproteinases-1, or GM6001 inhibits the C3-induced migration. Urokinase and its receptor are detected and unchanged upon exposure to C3. The antibody against urokinase or addition of plasminogen activator inhibitor inhibits migration. Blocking antibodies to integrins ␣ 2 , ␣ 6 , ␤ 1 , and ␤ 3 inhibit chemotaxis and do not change urokinase and urokinase receptor expression. Blockage of ␣ 2 , ␤ 1 , and ␤ 3 integrins affect differently the induction by C3 of pro-metalloproteinase-2 and -9 and of tissue inhibitor of metalloproteinases-2. Chemotaxis to C3 is also inhibited by genistein, by pertussis toxin, which also inhibits C3-induced pro-metalloproteinase -2 and -9, but not urokinase expression. Wortmannin partially inhibits C3-induced cell migration. Other, but not all, breast carcinoma lines tested responded to C3 with migration and pro-metalloproteinase-2 induction. Presently C3 is the only agent known to induce migration specifically of both endothelial and breast carcinoma cells. The mitogenic and motogenic role of C3 in vitro might prefigure a role in in vivo carcinogenesis and in the establishment of metastasis.Mature osteoblasts conditioned medium (CM), 1 collected when sustained biosynthesis of collagen type I occurs during osteogenesis in vitro from differentiating cultures of rat, rabbit, human osteoblasts, and conditionally transformed human osteoblast-like line HFO, induces directional migration of endothelial and melanoma and breast and prostatic carcinoma cells but not of normal cells or of transformed mesenchymal cells in the classical in vitro double chamber Boyden assay (1-4).The molecule chemoattractant for endothelial cells was purified from rat osteoblast CM, and it identifies by sequence, immunoreactivity, and molecular size with the trimer carboxylterminal of type I pro-collagen, C3 (4). C3 is a by-product of the processing of pro-collagen by pro-collagenase C (or BMP-1), preliminary to the formation of collagen fibrils (5).We investigated whether MDA MB231 breast carcinoma cells, which are stimulated by mature osteoblasts CM to directional migration, are induced by C3 to activate migratory and proliferation responses. It is recognized that the stroma of the tumors plays instructive and active roles toward tumor cells growth and invasiveness and that tumor-associated stromal fibroblasts undergo changes in the biosynthetic phenotype (6), assume the production of factors supp...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Pro-collagen I COOH-terminal Trimer Induces Directional Migration and Metalloproteinases in Breast Cancer Cells

Palmieri¹,

Poggi²,

Ulivi³

et al. 2003

Journal of Biological Chemistry

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“…The evidence for this includes observations that bisphosphonates reduce IL-6 expression by osteoblasts (39) and induce soluble inhibitors of osteoclast activity into conditioned media (40). Futhermore, osteoblasts express factors that promote the growth of prostate cancer cells but co-culture of tumour cells and osteoblasts suggest that ZOL is capable of directly inhibiting growth of prostate cancer cells even when grown in the presence of an osteoblast cell line (41,42). However, bisphosphonates may also inhibit osteoclastic bone resorption through increased expression of factors such as osteoprotegerin by normal human osteoblasts (43).…”

Section: Mechanism Of Apoptotic Deathmentioning

confidence: 99%

Antitumor Effects of Aminobisphosphonates on Renal Cell Carcinoma Cell Lines

et al. 2006

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PurposeBisphosphonates are established as a supportive therapy for a number of malignancies which metastasise to bone. Previous reports also suggest potent antitumour and antiangiogenic properties. We investigated the in vitro activity of two aminobisphosphonates, pamidronate (PAM) and zoledronic acid (ZOL) on the growth and survival of three renal cell carcinoma cell lines (Caki-2, 769-P and D69581). Experimental design

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“…9,11 It was hypothesized that osteoblast products, directly secreted or released from the bone matrix by osteoclasts resorbing activity, are potential chemoattractive agents for bone colonizing tumor cells. 12 This hypothesis was tested using an in vitro system of differentiating osteoblasts. The data demonstrated that osteoblast products found in conditioned media were able to attract breast and melanoma tumor cells.…”

Section: Chemoattractantsmentioning

confidence: 99%

Cell adhesion and chemotaxis in prostate cancer metastasis to bone: a minireview

Cooper

2000

Prostate Cancer Prostatic Dis

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Bone metastasis is a common phenomenon in patients with advanced prostate cancer. The molecular and cellular mechanisms involved in this process are not well understood. Past reviews on this subject primarily focused on prostate tumor growth in the bone marrow and the effects this growth has on bone homeostasis (ie osteoblastic and osteolytic). Cell chemotaxis and adhesion are also important for site-speci®c metastasis. In this review we have focused on chemotactic and cell adhesion molecules potentially involved in prostate cancer metastasis to bone. In addition, recently developed animal models for prostate cancer metastasis to bone are discussed. Prostate Cancer and Prostatic Diseases (2000) 3, 6±12.

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Effect of osteoblast supernatants on cancer cell migration and invasion

Cited by 20 publications

References 7 publications

Pro-collagen I COOH-terminal Trimer Induces Directional Migration and Metalloproteinases in Breast Cancer Cells

Pro-collagen I COOH-terminal Trimer Induces Directional Migration and Metalloproteinases in Breast Cancer Cells

Antitumor Effects of Aminobisphosphonates on Renal Cell Carcinoma Cell Lines

Cell adhesion and chemotaxis in prostate cancer metastasis to bone: a minireview

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