Effect of Osmolarity on Virulence of Uropathogenic Pseudomonas Aeruginosa

Abstract

In the milieu of urinary tract, urine is subject to changes in osmolarity depending on host's diet and clinical condition. The present investigation was planned to study the effect of osmolarity on growth and elaboration of virulence factors by Pseudomonas aeruginosa in planktonic and biofilm cell mode. Significant enhancement in growth and elaboration of all the virulence traits was observed with increase in osmolarity of the growth medium from 100 to 300 mOsmol/l. However, with further increase in osmolarity to 350 mOsmol/l there was significant decrease in growth and production of all the virulence determinants. In addition, organisms grown in 300 mOsmol/l medium showed increased adherence to uroepithelial cells, evade phagocytosis and were more virulent in mouse model of ascending pyelonephritis as indicated by higher bacterial load and tissue damage. The findings of the present study may be relevant for the better understanding of host-parasite interactions and will pave the way for the future development of preventive strategies against urinary tract infections.

Exogenous maltose enhances Zebrafish immunity to levofloxacin‐resistant Vibrio alginolyticus

“…This is the first demonstration, to our knowledge, that metabolomic strategy differs in the innate immune response to antibiotic‐resistant and antibiotic‐sensitive bacteria. Alternatively, injection of ZF with maltose may alter the osmolarity of ZF internal fluids (Mittal et al , 2009), which could alter tissue surface tension and alter the interaction between pathogen and host. This possibility awaits further investigation (Krens et al , 2017).…”

Urinary tract infections caused by Pseudomonas aeruginosa: A minireview

“…In order to establish and cause UTI, P. aeruginosa has to adapt itself to variations in osmolarity of urine. We observed that osmolarity has profound influence on urovirulence of P. aeruginosa [111]. There was significant increase in production of virulence factors with increase in osmolarity from 200 to 300 mOsmol/l.…”

Pseudomonas aeruginosa: patogenicidad y resistencia antimicrobiana en la infección urinaria

“…Mittal y cols., demostraron en el año 2009 que condiciones de osmolaridad de 200 a 300 mOsmol/l. incrementaban la patogenicidad de P. aeruginosa, aumentaban la resistencia a la fagocitosis y se correlacionaba con la producción de malondialdehido (MDA), siendo éste un marcador de daño tisular (renal y de vejiga urinaria) 66 .…”

“…Existen factores, como la pérdida de la integridad tisular, que se han reconocido como facilitadores de la formación de biopelícula; lo anterior puede ocurrir al introducir catéteres en vía urinaria 66 .…”