Effect of original anticonvulsant meta-chloro-benzhydryl-urea on behavioral and immune parameters in mice with active and passive behavior types in experimental alcoholism

Abstract

ObjectiveViolation of the functional activity of the nervous and immune systems is an essential link in the pathogenesis of chronic alcohol dependence. The search for new psychopharmacological agents whose action is directed to correction of neuroimmune interaction opens new perspectives for the treatment of alcohol dependence.Methods(CBAxC57Bl/6) F1 mice with active and passive behavioral types in a state of chronic alcohol dependence owing to 6 month 10% ethanol exposure were undergoing intragastric administration of original anticonvulsant meta-chloro-benzhydryl-urea. Animal's behavioral and immune parameters, brain cytokines synthesis before and after anticonvulsant receiving were estimated.ResultsIn the formation of experimental alcohol dependence in animals the most pronounced changes in motor and exploratory activities in open field test, brain cytokines synthesis and suppression of immune response were registered in mice with passive type of behavior. Daily consumption of ethanol solution in mice with chronic alcohol dependence decreased sharply starting from 2 days of anticonvulsant administration and led to the cessation of ethanol consumption by the 5 day. After anticonvulsant administration for 10 days behavioral parameters in mice were comparable with those in the control group of healthy animals. It also restored brain cytokines synthesis and significant stimulated humoral immune response, estimated by the relative number of antibody-forming spleen cells.ConclusionBehavior and immune changes following chronic ethanol exposure depended on the behavior status of animals; administration of the original anticonvulsant meta-chloro-benzhydryl-urea may correct both immune and behavior disorders in mice with chronic alcohol dependence, so it has promise in the treatment of alcoholism.Disclosure of interestThe authors have not supplied their declaration of competing interest.

Turmeric Extract in Correction of Nervous and Immune Systems Functional Activity Parameters in Experimental Alcoholism

“…Control groups: healthy mice and alcoholic animals, received water or 10% ethanol solution, 5 ml per animal per day. Animals of the experimental group took turmeric extract with a final ethanol concentration of 10%, in the same volume, daily, for 2.5 months, then all animals were evaluated for behavior parameters in the "open field" test and the severity of the cellular immune response by the intensity of the development of a delayedtype hypersensitivity reaction (DTH), according to the methods described earlier [6,7]. Statistical processing of the results was performed using the Statistica 7.0 software package (StatSoft, USA) using the non-parametric Mann-Whitney test.…”