Effect of Organic Fe-Ligands, Released by Emiliania huxleyi, on Fe(II) Oxidation Rate in Seawater Under Simulated Ocean Acidification Conditions: A Modeling Approach

Abstract

The potential effect of ocean acidification on the exudation of organic matter by phytoplankton and, consequently, on the iron redox chemistry is largely unknown. In this study, the coccolithophorid Emiliania huxleyi was exposed to different pCO 2 conditions (225-900 µatm), in order to determine the role of natural organic ligands on the Fe(II) oxidation rate. Oxidation kinetics of Fe(II) were studied as a function of pH (7.75-8.25) and dissolved organic carbon levels produced (0-141.11 µmol C L −1 ) during the different growth stages. The Fe(II) oxidation rate always decreased in the presence of exudates as compared to that in the exudates-free seawater. The organic ligands present in the coccolithophorid exudates were responsible for this decrease. The oxidation of Fe(II) in artificial seawater was also investigated at nanomolar levels over a range of pH (7.75-8.25) at 25 • C in the presence of different glucuronic acid concentrations. Dissolved uronic acids (DUA) slightly increased the experimental rate compared to control artificial seawater (ASW) which can be ascribed to the stabilization of the oxidized form by chelation. This behavior was a function of the Fe(II):DUA ratio and was a pH dependent process. A kinetic model in ASW, with a single organic ligand, was applied for computing the equilibrium constant (log K FeCHO+ = 3.68 ± 0.81 M −1 ) and the oxidation rate (log k FeCHO+ = 3.28 ± 0.41 M −1 min −1 ) for the Fe(II)-DUA complex (FeCHO + ), providing an excellent description of data obtained over a wide range of DUA concentrations and pH conditions. Considering the Marcus theory the Fe(III) complexing constant with DUA was limited to between 10 13 and 10 16 . For the seawater enriched with exudates of E. huxleyi a second kinetic modeling approach was carried out for fitting the Fe(II) speciation, and the contribution of each Fe(II) species to the overall oxidation rate as a function of the pH/pCO 2 conditions. The influence of organic ligands in the Fe(II) speciation diminished as pH decreased in solution. During the stationary growth phase, the FeCHO + complex became the most important contributor to the overall oxidation rate when pH was lower than 7.95. Because CO 2 levels modify the composition of excreted organic ligands, the redox behavior of Fe in solution may be affected by future acidification conditions.

Fe(II) stability in seawater

“…Microcystis aeruginosa (Lee et al, 2017), coccolithophore Emiliania huxleyi (Samperio-Ramos et al, 2018a), and diatoms Chaetoceros radicans (Lee et al, 2017) and Phaeodactylum tricornutum (Santana-Casiano et al, 2014) have all been found to retard Fe(II) oxidation rates. Furthermore, the effect of cellular exudates on the reaction constant appears to scale with increasing total organic carbon (Samperio-Ramos et al, 2018b).…”