Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas

Ruiz, J.; Santayana, R. Paulo; Mendoza, Miriam; Landeo, J Leandra; Huaman, E.; Ticllacuri, Flamel; Mujica, L. Fidel; Silva, Mauricio; Ratto, M.H.

doi:10.1016/j.theriogenology.2017.03.006

Cited by 13 publications

(24 citation statements)

References 38 publications

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“…These results confirmed that swim-up is not an adequate method of sperm selection in llamas, even after collagenase treatment. However, this technique gave acceptable results when used on alpaca epididymal sperm ( 12 ). These authors did not report the number of motile spermatozoa recovered, but they obtained embryos by IVF using swim-up in alpaca epididymal sperm.…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

“…This gamete can be collected from dead or live animals. The methods used to obtain cumulus-oocyte complexes (COCs) are: aspiration or dissection of follicles from slaughterhouse ovaries ( 9 – 12 ); aspiration of follicles surgically exposed during a laparotomy [llamas ( 13 – 16 ); alpacas ( 17 )] or ultrasound-guided transvaginal aspiration of follicles ( 18 – 21 ).…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

“…Del Campo et al published the first report of IVM of llama oocytes in 1992. Since then, based on oocytes reaching the metaphase II stage (MII) of development, most researchers have reported 39–80% nuclear maturation of oocytes between 24 and 36 h [llamas ( 11 , 23 ); alpacas ( 12 )]. Evaluation of cytoplasmic maturation, however, has not been reported for SAC oocytes coming from slaughterhouse ovaries.…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

“…Evaluation of cytoplasmic maturation, however, has not been reported for SAC oocytes coming from slaughterhouse ovaries. Nonetheless, there does appear to be a consensus that llama oocytes from slaughterhouse ovaries need approximately 36 h in culture to mature to the MII stage ( 11 , 23 ), and in the case of alpacas, 32 h resulted in the highest rate of oocytes reaching the MII stage ( 12 ). Furthermore, as oocytes were recovered from an abattoir, it is unknown if the dominant follicles were growing or regressing, a fact that would affect oocyte quality ( 24 ).…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

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Production, Preservation, and Transfer of South American Camelid Embryos

et al. 2017

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The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in vitro embryo production). Moreover, superstimulation entails prior administration of hormones that inhibit follicular growth (progesterone, progestagens, and estrogens). Cumulus-oocyte complexes obtained must mature in vivo (buserelin administration) or in vitro to then be subjected to in vitro fertilization or intracytoplasmic sperm injection. All these techniques also require morphologically normal, motile spermatozoa to achieve fertilization. Methods used to decrease semen viscosity and to select the best spermatozoa (Percoll®; Androcoll-ETM) are described. Additionally, nuclear transfer or cloning has been applied in llamas. Up to now, embryo deep-freezing and vitrification have progressed slowly but are at the height of development. Embryos that are obtained by any of these techniques, either in vivo or in vitro, need to be transferred to synchronized recipient females. The best results are achieved after transfer to the left uterine horn with an ipsilateral ovulation. No live offspring have been obtained after the transfer of cryopreserved embryos. Applying reproductive biotechnologies, such as those described, will permit the expansion of genetically selected animals in the population and also that of wild camelid species, vicunas, and guanacos, whose embryos could then be transferred to the uterus of domestic species.

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Section: In Vitro Embryo Productionmentioning

confidence: 99%

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Section: In Vitro Embryo Productionmentioning

confidence: 99%

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Production, Preservation, and Transfer of South American Camelid Embryos

et al. 2017

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“…This species possesses interesting physiological and reproductive characteristics such as induced ovulation (Bravo et al, ) and the presence, in the male seminal plasma, of an ovulation‐inducing factor (OIF; Kershaw‐Young and Maxwell, ; Kershaw‐Young et al, ; Stuart et al, ). For these reasons, past research has been mainly focused on reproductive mechanisms (Bravo et al, ; Correa et al, ; Ratto et al, , , ; Ruiz et al, ). The study of early morphogenesis in Vicugna pacos became more important after the introduction of reproductive techniques such as in vitro embryo production (Trasorras et al, ).…”

mentioning

confidence: 99%

Mapping of the Early Intrauterine Morphogenesis in the Alpaca (Vicugna pacos): External Features and Development of the Cephalic Vesicle in Comparison with the Progressive Carnegie Scale

Montelli

Stelletta

Ruiz

et al. 2018

The Anatomical Record

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In this study, we characterized the morphological aspects of the early development of the head of the alpaca (Vicugna pacos) and identified the main structures of the central nervous system during the first trimester of pregnancy. The topography and the cytoarchitecture of the fetal brain regions were described by histological analysis of the brain sections. We performed this analysis on alpaca embryos and fetuses presumably aged 20, 30, 45, and 90 days. For the description of the external body structures we considered the shape of the head, the development of the optic primordium, the dorsal curvature of the body, the limb buds, the umbilical cord and relative vessels, and the thickness and transparency of the skin. The prosencephalic, mesencephalic, and the rhomboencephalic vesicles were described by analyzing sagittal sections of the head. The present article provides the first progressive morphological and anatomical description of alpaca brain during early development. A detailed study represents an important basis to further understand the phases of prenatal development in this species, since information about alpaca embryology in incomplete and reproductive failure is a relevant factor. These data are important also for interspecies comparisons and application of reproductive biotechnologies. Anat Rec, 302:1226–1237, 2019. © 2018 Wiley Periodicals, Inc.

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