Effect of oocyte denudation timing on clinical outcomes in 1212 oocyte recipients

Esbert, Marga; Florensa, M.; Riqueros, M.; Teruel, José Romero; Ballesteros, A.

doi:10.1016/j.fertnstert.2013.07.390

Cited by 2 publications

(2 citation statements)

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“…Therefore, separating the CCs from COC for ICSI preparation is technically challenging. To date, the denudation process is conducted in two phases [18,32]. The first step was that the COCs would be enzymolyzed in hyaluronidase, such as HYASE-10X™ (Vitrolife® Sweden), to weaken the bond of HA within the CCs and oocytes.…”

Section: Discussionmentioning

confidence: 99%

RNA Extraction from Cumulus Cells for Gene Analysis: The Current Strategy and Review of Literature

Ahmad,

Elias,

Mat Jin

et al. 2024

Preprint

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RNA extraction from human cumulus cells (CCs) is considered challenging due to low cell’s pop-ulation and small cell. The successful gene expression analysis from cumulus cells ultimately de-pends on an excellent concentration of Ribonucleic acid (RNA) yield by an appropriate technique. Our study aims to consolidate appropriate cumulus cell collection method, preparation and ex-traction techniques to ensure a good yield of RNA for better gene expression analysis. From the results obtained, the optimal strategy found involved mechanical denudation without an enzy-matic process, followed by the use of RNA stabilizer prior to RNA extraction. Subsequently, uti-lizing a smaller homogenizer, preferably manual pressure control with filter (Bio-Masher III®) is paramount for a pure and homogenize sample. Subsequently, during RNA extraction, the use of RNeasy micro kit and RNA carriers is recommended. These sample collection, preparation, and extraction techniques yielded an excellent-quality RNA concentration and successful gene expres-sion analysis. We also conducted a systematic review to consolidate our findings with current evidence of RNA extraction technique. Our results added a value as a current strategy for opti-mizing RNA extraction in CCs for experimental studies. Thus, our findings can contribute to for-mulating a better strategy for optimizing the RNA extraction in CCs for transcriptomic studies.

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Section: Discussionmentioning

confidence: 99%

RNA Extraction from Cumulus Cells for Gene Analysis: The Current Strategy and Review of Literature

Ahmad,

Elias,

Mat Jin

et al. 2024

Preprint

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show abstract

“…Many studies compared different time intervals between OPU-DN and concluded that oocyte maturity remarkably improved with the increase in OPU-DN time. [ 18 19 20 ] Furthermore, Hassan observed that oocyte maturation was remarkably lower with immediate denudation than with 4-h incubation with intact cumulus (80.5% vs. 91.9%).…”

Section: Discussionmentioning

confidence: 99%

Two-six hours is the optimal timing of intracytoplasmic sperm injection after oocyte pickup: Single-centre 10 years cohort study

et al. 2022

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Effect of oocyte denudation timing on clinical outcomes in 1212 oocyte recipients

Cited by 2 publications

References 0 publications

RNA Extraction from Cumulus Cells for Gene Analysis: The Current Strategy and Review of Literature

RNA Extraction from Cumulus Cells for Gene Analysis: The Current Strategy and Review of Literature

Two-six hours is the optimal timing of intracytoplasmic sperm injection after oocyte pickup: Single-centre 10 years cohort study

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