Effect of omega-3 fatty acids on haemostatic functions in urocortin-treated obese rats

Elgendy, Ahmed; Abbas, Amr M.

doi:10.1007/s13105-014-0350-3

Cited by 9 publications

(7 citation statements)

References 59 publications

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“…APTT and PT were marginally shorter in the HFD-fed animals for both sexes. Contrary to previous studies (Kaji et al 2013; El-Gendy and Abbas 2014; Dallak et al 2018; Sakr, Abbas, and Haidara 2018), HFD-fed rats exhibited a decrease in both FIB and PLT counts, which may reflect a low-grade consumption of both parameters owing to a mild hypercoagulable state associated with chronic HFD feeding. These findings suggest that the HFD-fed animals may have a higher tendency for increased blood clotting.…”

Section: Discussioncontrasting

confidence: 99%

The Effect of Diet-induced Obesity on Toxicological Parameters in the Polygenic Sprague-Dawley Rat Model

et al. 2018

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The obese rodent serves as an indispensable tool for proof-of-concept efficacy and mode-of-action pharmacology studies. Yet the utility of this disease model as an adjunct to the conventional healthy animal in the nonclinical safety evaluation of anti-obesity pharmacotherapies has not been elucidated. Regulatory authorities have recommended employing disease models in toxicology studies when necessary. Our study investigated standard and exploratory toxicology parameters in the high-fat diet (HFD)-induced obese, polygenic Sprague-Dawley rat model in comparison to chow diet (CD)-fed controls. We sought to establish feasibility of the model for safety testing and relevance to human obesity pathophysiology. We report that both sexes fed a 45% kcal HFD for 29 weeks developed obesity and metabolic derangements that mimics to a certain extent, common human obesity. Minor clinical pathologies were observed in both sexes and considered related to CD versus HFD differences. Histopathologically, both sexes exhibited mild obesity-associated findings in brown and subcutaneous white fat, bone, kidneys, liver, lung, pancreas, salivary parotid glands, and skeletal muscle. We conclude that chronic HFD feeding in both sexes led to the development of an obese but otherwise healthy rat. Therefore, the diet-induced obese Sprague-Dawley rat may serve as a suitable model for evaluating toxicity findings encountered with anti-obesity compounds.

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Section: Discussioncontrasting

confidence: 99%

The Effect of Diet-induced Obesity on Toxicological Parameters in the Polygenic Sprague-Dawley Rat Model

et al. 2018

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“…5) in isoproterenolinduced rats. These results were in agreement with those of previous studies [8,25]. Moreover, the improvement of the serum TG by n-3 PUFA may explain the additive effect observed for ISOR + n-3 PUFA.…”

Section: Discussionsupporting

confidence: 93%

“…Group II included normal rats administered ISOR (10 −6 mol/L, in drinking water) [41] for 30 days. Group III included normal rats administered n-3 PUFA (0.3 g/kg/day by gavage) [8] for 30 days. Group IV animals (AMI) received isoproterenol (85 mg/kg body weight) intraperitoneally twice at an interval of 24 h on the 29th and 30th days.…”

Section: Experimental Designmentioning

confidence: 99%

Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats

Abbas

2016

J Physiol Biochem

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Myocardial infarction (MI) is a common cause of mortality worldwide. Isorhapontigenin is a derivative of stilbene with chemical structure similar to resveratrol. The omega-3 fatty acids (FA) have beneficial effects on neurodegenerative, inflammatory, and cardiovascular diseases. The aim of this study was to investigate the effects of pretreatment with isorhapontigenin and omega-3 FA on rat model of isoproterenol-induced MI. Fifty-six rats were divided into seven groups: normal, normal + isorhapontigenin, normal + omega-3 FA, MI, MI + isorhapontigenin, MI + omega-3 FA, and MI + isorhapontigenin + omega-3 FA. Serum levels of cardiac marker enzymes [lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB)], cardiac troponin I (cTnI), inflammatory markers [tumor necrosis factor-alpha (TNF-α) and interleukin-6], and lipid profile [triglycerides, total cholesterol (T.Ch), high and low density lipoproteins (HDL, LDL), and phospholipids] as well as cardiac levels of malondialdehyde and anti-oxidants [reduced glutathione (GSH), superoxide dismutase (SOD), and catalase)] were measured in all rats. ECG and histopathological examination were performed. Isoproterenol caused a significant elevation of ST segment, decreased R wave amplitude, HDL, and anti-oxidants, and increased LDH, CK-MB, cTnI, TNF-α, interleukin-6, malondialdehyde, triglycerides, T.Ch, LDL, and phospholipids. Omega-3 FA or isorhapontigenin significantly decreased the ST segment elevation, LDH, CK-MB, cTnI, TNF-α, interleukin-6, malondialdehyde, and phospholipids and increased R wave amplitude and anti-oxidants. The effects of combined omega-3 FA and isorhapontigenin were more significant than either of them alone. Therefore, we conclude that omega-3 FA and isorhapontigenin have a cardioprotective effect on rats with isoproterenol-induced MI through their anti-oxidant and anti-inflammatory actions.

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“…Following these early results, several mechanistic studies of EPA and DHA and platelet function suggested diminished platelet aggregation, with a variety of proposed molecular mechanisms. 37–43 These experimentally observed effects raised concern about a potential bleeding risk with fish oil supplements, 8, 11 which has led many health care providers and organizations to request that patients should stop taking fish oil for at least one week before surgery. 9, 10, 44, 45 This standard of practice can therefore delay patient care as well as increase hospital length of stay and cost.…”

Section: Discussionmentioning

confidence: 99%

Fish Oil and Perioperative Bleeding

Akintoye

Sethi

Harris

et al. 2018

Circ: Cardiovascular Quality and Outcomes

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Background: Fish oil is among the most common natural supplements for treatment of hypertriglyceridemia or prevention of cardiovascular disease. However, ongoing concerns about possible bleeding risk has led to recommendations that patients stop taking fish oil prior to surgery and/or delay in elective procedures for patients taking fish oil Methods and Results: We tested the effect of fish oil supplementation on peri-operative bleeding in a multinational, placebo-controlled trial involving 1516 patients who were randomized to perioperative fish oil (EPA + DHA; 8–10 g over 2–5 days preoperatively, then 2 g/day postoperatively) or placebo. Primary outcome was major peri-operative bleeding as defined by the Bleeding Academic Research Consortium (BARC). Secondary outcomes include peri-operative bleeding per TIMI and ISTH definitions, chest tube output, and total units of blood transfused. Mean(SD) participants age was 63(13) years, and planned surgery included CABG (52%) and valve surgery (50%). The primary outcome occurred in 92 patients (6.1%). Compared to placebo, risk of BARC bleeding was not higher in the fish oil group: OR=0.81, 95%CI=0.53–1.24; absolute risk difference=1.1% lower (95%CI= −3.0 to 1.8%). Similar findings were seen for secondary bleeding definitions. The total units of blood transfused were significantly lower in the fish oil group compared to placebo (mean=1.61 vs 1.92, p<0.001). Evaluating achieved plasma phospholipid n-3-PUFA levels with supplementation (on the morning of surgery), higher levels were associated with lower risk of BARC bleeding, with substantially lower risk in the third (OR=0.30 [95%CI, 0.11–0.78]) and fourth (0.36 [95%CI, 0.15–0.87]) quartiles, compared with the lowest quartile. Conclusions: Fish oil supplementation did not increase peri-operative bleeding; and reduced the number of blood transfusions. Higher achieved n-3-PUFA levels were associated with lower risk of bleeding. These novel findings support the need for reconsideration of current recommendations to stop fish oil or delay procedures prior to cardiac surgery. Clinical Trial Registration URL: http://Clinicaltrials.gov Unique identifier: NCT00970489

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Effect of omega-3 fatty acids on haemostatic functions in urocortin-treated obese rats

Cited by 9 publications

References 59 publications

The Effect of Diet-induced Obesity on Toxicological Parameters in the Polygenic Sprague-Dawley Rat Model

The Effect of Diet-induced Obesity on Toxicological Parameters in the Polygenic Sprague-Dawley Rat Model

Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats

Fish Oil and Perioperative Bleeding

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