Effect of oligosaccharides extract from palm kernel expeller on growth performance, gut microbiota and immune response in broiler chickens

Abstract

This study examined the prebiotic effects of oligosaccharides extract from palm kernel expeller (OligoPKE) on growth performance, cecal microbiota and immune response of broiler chickens. A total of ninety 1-day-old broiler chicks (Cobb-500) were randomly allocated to three treatment groups of six pens (replicates) with five birds per pen. Dietary treatments were: (i) basal diet as control, (ii) basal diet plus 0.5% OligoPKE, and (iii) basal diet plus 1% OligoPKE. Birds growth traits (ADG, ADFI and G:F) were measured during the starter (1-21 day), finisher (22-35 day) and the entire experimental periods. Blood and cecal digesta samples were collected from chickens at 21 and 35 days of age (DOA). Microbial quantification of the digesta samples, white blood cells including heterophil, lymphocyte, monocyte, eosinophil, basophil counts and immunoglobulin (IgA and IgM) were also determined. OligoPKE had no effect on ADG and ADFI throughout the study period, but chickens fed OligoPKE supplemented diet had better (P < 0.05) G:F during finisher and overall rearing periods. Supplementing OligoPKE did not significantly alter the birds' microbiota of the cecal digesta. At 21 DOA, blood IgA concentration increased significantly when birds fed 1% OligoPKE in diet recorded compared to the control treatment. Similar observations were also recorded in birds at 35 DOA. Hematological data showed that heterophil and basophil counts of chickens fed OligoPKE supplement were lower than those in control group at 21 DOA. Our findings suggested that OligoPKE improved immune responses in broiler chickens, especially at younger age when the immune system is not still fully developed.

Effect of olive meal and supplemental enzymes on performance traits, blood biochemistry, humoral immunity response and caecal microbiota of broilers

“…Previous studies have focused on the addition of supplemental enzymes, yeast and other probiotics and, depending on the study, some beneficial effects were found (Dhama et al, 2015;Rezaei et al, 2015;Alefzadeh et al, 2016). In the current study, the numbers of aerobic, lactic acid-producing, and coliform bacteria did not differ (P >0.05) among experimental groups, demonstrating the safety of adding OM to both animal and human populations.…”

Prebiotic effects of oligosaccharides extracted from palm kernel expeller on different levels of <i>Salmonella typhimurium</i> infection in chicks

“…Oligosaccharides were extracted from raw palm kernel expeller (PKE) using water (without enzyme) as solvent, following the procedure of Rezaei et al (2015). Briefly, 1 L distilled water was added to 200 g ground PKE in a one-litre Scott bottle, shaken for 1 hour at room temperature, and later autoclaved.…”

Oligosaccharides from Palm Kernel Cake Enhances Adherence Inhibition and Intracellular Clearance of Salmonella enterica Serovar Enteritidis In Vitro

“…While MOS does not have the GRAS status, animal trials with MOS supplementation at a dose of 1% (10 mg/mL) showed no adverse effect [42,43]. Likewise, no adverse effects were observed in in vivo trials when oligosaccharides from PKC at a concentration of 10 mg/mL were used as supplements [44,45].…”