Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats

Pereira, Leonardo; Hatanaka, Elaine; Martins, Edgair Fernandes; Oliveira, Flávia de; Liberti, Édson Aparecido; Farsky, Sandra Helena Poliselli; Curi, Rui; Pithon-Curi, Tânia Cristina

doi:10.1002/cbf.1432

Cited by 154 publications

(122 citation statements)

References 39 publications

(41 reference statements)

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“…Previous studies by Cardoso et al (2004) and Pereira et al (2008) indicated acceleration in the wound healing process in rats treated with n-6 fatty acids, as well as a tendency toward faster wound closure in rats receiving n-9 fatty acids. A tendency for delayed wound closure in rats treated with n-3 fatty acids was also observed.…”

Section: Introductionmentioning

confidence: 83%

“…The animals were housed in cages (maximum of five per cage) maintained at 23 °C under a lighting regimen of 12/12 h. Food and water were given ad libitum (Pereira et al, 2008). All experiments were conducted according to the guidelines of the Animal Care Committees of the Institute of Biomedical Sciences and the Faculty of Pharmaceutical Sciences of the University of São Paulo (Protocol Numbers CEEA ICB/ USP 68-71-2 and CEEA FCF/USP 236).…”

Section: Animalsmentioning

confidence: 99%

“…After rinsing the shaved surface with ethanol at 70%, an excision wound was made by cutting out a 1.0 cm x 1.0 cm piece of skin from the shaved area. The wounds were of the full-thickness type extending down to the subcutaneous tissue (Pereira et al, 2008).…”

Section: Wound Inductionmentioning

confidence: 99%

“…This protocol was established based on the protocols used by Cardoso et al (2004) and Pereira et al (2008).…”

Section: Treatmentsmentioning

confidence: 99%

“…In the most conclusive studies regarding the regulating effect of omega-3 (n-3), omega-6 (n-6), and omega-9 (n-9) fatty acids on immune cell functions, these fatty acids have been either topically administered or supplemented in their free form and at a very high degree of purity (Pereira et al, 2008;Rodrigues et al, 2010). Other groups have used blends of these fatty acids or edible vegetable or animal oils (Nevin,Rajamohan, 2010;McDaniel et al, 2008;Otranto, Nascimento, Monte-AltoCosta, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Production of vegetable oil blends and structured lipids and their effect on wound healing

Ract

Soares

Rodrigues

et al. 2015

Braz. J. Pharm. Sci.

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Two oil blends (sunflower/canola oils 85/15 (BL1) and canola/linseed oils 70/30 (BL2)), were prepared and enzymatically interesterified to be applied to surgically-induced wounds in rats. Following surgery, the animals were submitted to the Treatment with Physiological Saline (TPS) (control group), Blends (TBL), and Structured Lipids (TSL). The control group (TPS) received physiological saline solution for 15 days. In TBL, BL1 was administered during the inflammation phase (days 0-3) and BL2 in the tissue formation and remodeling phase (days 4-15). In TSL, Structured Lipid 1 (SL1) and Structured Lipid 2 (SL2) were used instead of BL1 and BL2, respectively. The aim of this study was to compare wound closure evolution among rats treated with the blends or structured lipids versus control rats treated with physiological saline. The wound healing process was evaluated by measuring the wound areas along the treatments and the concentrations of cytokines. An increase in the areas of wounds treated with the blends and structured lipids in the inflammatory phase was observed, followed by a steeper closure curve compared to wounds treated with physiological saline. The changes observed during the inflammatory phase suggest a potential therapeutic application in cutaneous wound healing which should be further investigated.Uniterms: Structured lipid/effect/wound healing. Vegetable oil/effect/wound healing. Healing. Cutaneous wound healing/treatment. Duas misturas de óleos vegetais (girassol/canola 85/15 (BL1) e canola/linhaça, 70/30 (BL2) foram preparadas e interesterificadas por via enzimática para serem aplicadas em feridas induzidas cirurgicamente em ratos. Após a cirurgia, os animais foram submetidos ao tratamento com soro fisiológico (TPS) (grupo controle), tratamento com as misturas (TBL) e tratamento com os lipídios estruturados (TSL). O grupo controle (TPS) recebeu soro fisiológico por 15 dias. Em TBL, BL1 foi administrada durante a fase de inflamação (dias 0-3) e BL2 na fase de formação de tecido e remodelação (dias 4-15). Em TSL, os lipídios estruturados SL1 e SL2 foram usados em vez de BL1 e BL2, respectivamente. O objetivo deste estudo foi avaliar a evolução do fechamento das feridas dos grupos de ratos tratados com as misturas ou lipídios estruturados em comparação com os ratos do grupo controle, tratados com soro fisiológico. O processo de cicatrização das feridas foi avaliado através da medição das áreas das feridas ao longo dos tratamentos e pela determinação das concentrações de citocinas. Observou-se aumento das áreas das feridas tratadas com as misturas e os lipídios estruturados na fase inflamatória, seguida por um fechamento acentuado de feridas comparado com o tratamento com solução salina. As mudanças observadas durante a fase inflamatória sugerem uma potencial aplicação terapêutica na cicatrização de feridas cutâneas, fazendo-se necessárias investigações posteriores.Unitermos: Lipídio estruturado/efeito/cicatrização de feridas. Óleo vegetal/efeito/cicatrização de feridas. Cicatrização. Feridas cutân...

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Section: Introductionmentioning

confidence: 83%

Section: Animalsmentioning

confidence: 99%

Section: Wound Inductionmentioning

confidence: 99%

“…This protocol was established based on the protocols used by Cardoso et al (2004) and Pereira et al (2008).…”

Section: Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Production of vegetable oil blends and structured lipids and their effect on wound healing

Ract

Soares

Rodrigues

et al. 2015

Braz. J. Pharm. Sci.

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Chemical Profile of the Pits Oil from the Tunisian ‘Alig’ Cultivar of Phoenix dactylifera L.: In Vivo Wound Healing Potential Evaluation of a Cream Formulated from the Extracted Oil and Insights from Molecular Docking and SAR Analysis

Chhoud

Bagga

Lassoued

et al. 2022

Chemistry & Biodiversity

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Since ancient times the oil from date palm pits (Phoenix dactylifera L.) has been used to heal wounds. In order to prove this traditional usage of the pits, this oil was extracted from the pits of the Tunisian cultivar 'Alig' and its physico-chemical properties and the chemical composition were evaluated. The fatty acid profile, evidenced by GC, allowed to classify this oil as an oleic-myristic acid oil with a clear abundance of oleic acid (53.66 %). 1 H and 13 C-NMR as well as FT-IR analyses confirmed the presence of fatty acids in triglyceride forms. Furthermore, in vivo wound healing activity of a cream formulated from the extracted oil was performed, for the first time, using a rat model and was compared to placebo cream and a commercial formulation, MEBO®. This study showed that the test cream promoted the healing of pressure ulcers better than the placebo cream and the MEBO® ointment. The results showed that this vegetable oil is able to improve the healing of infected wounds in rats, thus supporting its traditional use. The contribution of the main oleic, linoleic and myristic acids that can be derived from enzymatic hydrolysis to the healing activity of the whole pits oil was predicted by in silico study and the calculated pharmacokinetics parameters.

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The effect of macadamia oil intake on muscular inflammation and oxidative profile kinetics after exhaustive exercise

Malvestiti¹,

Borges²,

Weimann³

et al. 2017

Euro J Lipid Sci & Tech

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In this study, we evaluated the onset and resolution of inflammation in control and macadamia oil‐supplemented rats after a single session of exhaustive exercise. We also analysed the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and thiobarbituric acid reactive substances (TBARS). Rats were supplemented for ten days with macadamia oil (25 mg/kg), which is rich in oleic and palmitoleic acids. On the 10th day, control and macadamia oil‐treated rats exercised to physical exhaustion. Plasma and muscular levels of pro‐inflammatory cytokines (tumour necrosis factor alpha [TNF‐α], interleukin‐1 beta [IL‐1β], IL‐6, cytokine‐induced neutrophil chemoattractant‐3 [CINC‐3], macrophage inflammatory protein‐3‐alpha [MIP‐3α]) and soluble L‐selectin were measured prior to, immediately after, and 2, 24, and 48 h after exercise. Our data revealed increases in the muscle concentrations of IL‐1β, L‐selectin, CINC‐3, and MIP‐3α in the group supplemented with macadamia oil compared with the concentrations in the control group. CAT also increased in the treated group, which is important because pro‐inflammatory cytokines precede growth factor production and might alter the muscle repair process. We concluded that inflammation resulting from exhaustive exercise was greater in animals that received macadamia oil than in control animals. Practical applications: There are potential benefits of using fatty acids in the treatment of injured muscles, and these substances have been used in several types of injured tissues. Macadamia oil intake alters muscle healing signaling by influencing the production of reactive oxygen species and inflammatory mediators. Therefore, it is reasonable to hypothesize that the intake of immunomodulatory supplements would be beneficial to the muscular recovery of an athlete following a strenuous exercise session. The muscle healing process has three phases: inflammation, new tissue formation and tissue remodeling, and these phases might be modulated by fatty acids. After an exhaustive exercise session, muscle inflammatory markers from macadamia oil‐supplemented rats are augmented in comparison to non‐supplemented animals. Our data revealed increases in the muscle concentrations of IL‐1β, L‐selectin, CINC‐3, and MIP‐3α in the group supplemented with macadamia oil compared with the concentrations in the control group.

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Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats

Cited by 154 publications

References 39 publications

Production of vegetable oil blends and structured lipids and their effect on wound healing

Production of vegetable oil blends and structured lipids and their effect on wound healing

Chemical Profile of the Pits Oil from the Tunisian ‘Alig’ Cultivar of Phoenix dactylifera L.: In Vivo Wound Healing Potential Evaluation of a Cream Formulated from the Extracted Oil and Insights from Molecular Docking and SAR Analysis

The effect of macadamia oil intake on muscular inflammation and oxidative profile kinetics after exhaustive exercise

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